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RISS 인기검색어
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- 저자 : 이철휘 ( Chul Hwi Lee ) (검색결과 2 건)
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홍상준,최가혜,송인선,이철휘,Hong, Sang-Joon,Choi, Ka-Hye,Song, In-Sun,Lee, Chul-Hwi 대한한방소아과학회 2010 대한한방소아과학회지 Vol.24 No.3
Objectives: The purpose of this study is to report two cases of adolescent patient with structural spinal scoliosis treated by Chuna therapy. Methods: We examined two adolescent patients with an X-ray examination. These patients were diagnosed with structural scoliosis, and we used acupuncture and Chuna method at these patients. Cobb's angle and VAS(Visual Analogue Scale) were used to evaluate effects of the treatment. Results and Conclusions: After the treatment, we got these improvement results : In one case, the Cobb's angle was improved by $8.4^{\circ}$ ($19^{\circ}$ to $10.6^{\circ}$) at thoracic level (44.2% correctability) and VAS has been changed from 5 to 1-2. In another case, the Cobb's angle was improved by $4.3^{\circ}$($11.3^{\circ}$ to $7^{\circ}$) at lumbar level(38.0% correctability) and VAS has been changed from 7 to 1-2.
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국화 SSR-enriched Library에서 SSR 반복염기의 분포 및 빈도
( Kyaw Thu Moe ),라상복 ( Sang Bog Ra ),이기안 ( Gi An Lee ),이명철 ( Myung Chul Lee ),박하승 ( Ha Seung Park ),김동찬 ( Dong Chan Kim ),이철휘 ( Cheol Hwi Lee ),최현구 ( Hyun Gu Choi ),전낙범 ( Nak Beom Jeon ),최병준 ( Byung J 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.5
Chrysanthemums, often called mums or chrysanths, belong to the genus Chrysanthemum, which includes about 30 species of perennial flowering plants in the family Asteraceae. We extracted DNA from Dendranthema grandiflorum (``Smileball``) to construct a simple sequence repeat (SSR)- enriched library, using a modified biotin-streptavidin capture method. GS FLX (Genome Sequencer FLX System which provides the flexibility to perform the broad range of applications) sequencing (at the 1/8 run specification) resulted in 18.83 mega base pairs (Mbp) with an average read length of 280.06 bp. Sequence analyses of all SSR-containing clones revealed a predominance of di-nucleotide motifs (16,375, 61.5%) followed by tri-nucleotide motifs (6,616, 24.8%), tetra-nucleotide motifs (1,674, 6.3%), pentanucleotide motifs (1,283, 4.8%), and hexa-nucleotide motifs (693, 2.6%). Among the di-nucleotide motifs, the AC/CA class was the most frequently identified (93.5% of all di-nucleotide types), followed by the GA/AG class (6.1%), the AT/TA class (0.4%), and the CG/GC class (0.03%). When we analyzed the distribution of different repeat motifs and their respective numbers of repeats, regardless of the motif class, of 100 SSR markers, we found a higher number of di-nucleotide motifs with 70 to 80 repeats; we also found two di-nucleotide motifs with 83 and 89 repeats, respectively, but their product lengths were within optimum size (297 and 300 bp). In future work, we will screen for polymorphisms of possible primer pairs. The results will provide a useful tool for assessing molecular diversity and investigating the population structure among and within Chrysanthemum species.
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