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역전사 (逆轉寫) 중합효소연쇄반응 (重合酵素連鎖反應) ( RT-PCR ) 과 제한효소 분석을 이용한 오이 모자이크 바이러스의 신속한 검정 및 동정
박원목(Won Mok Park),유기현(Ki Hyun Ryu),김수중(Su Joong Kim),최장경(Jang Kyung Choi) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.3
Based upon the nucleotide sequence of As strain of cucumber mosaic virus (CMV-As) RNA4, coat protein (CP) gene was selected for the design of oligonucleotide primers of polymerase chain reaction (PCR) for detection and identification of the virus. Reverse transcription and polymerase chain reaction (RT-PCR) was performed with a set of 18-mer CMV CP-specific primers to amplify a 671 bp fragment from crude nucleic acid extracts of virus-infected leaf tissues as well as purified viral RNAs. The minimum concentrations of template viral RNA and crude nucleic acids from infected tobacco tissue required to detect the virus were 1.0 fg and 1 : 65,536 (w/v), respectively. no PCR product was obtained when potato virus Y-VN RNA or extracts of healthy plants were used as templates in RT-PCR using the same primers. The RT-PCR detected CMV-Y strain as well as CMV-As strain. Restriction analysis of the two individual PCR amplified DNA fragments from CMV-As and CMV-Y strains showed distinct polymorphic patterns. PCR product from CMV-As has a single recognition site for EcoRI and EcoRV, respectively, and the product from CMV-Y has no site for EcoRI or EcoRV but only one site for HindIII. The RT-PCR was able to detect the virus in the tissues of infected pepper, tomato and Chinese cabbage plants.
박원목,김동수,박용환,정후섭,Park Won Mok,Kim Dong Soo,Park Yong Hwan,Chung Hoo Sup Korean Society of Applied Entomology 1971 한국식물보호학회지 Vol.10 No.2
본 시험은 우리나라에서 발생하는 푸른곰팡이병균의 종의 빈도와 발병환경 및 방제법을 구명코져 실시하였다. 시험결과 Trichoderma koningi, T. lignorum, T. glaucum과 미동정의 1종등 4종의 병원균이 분리되었고 이들의 빈도는 각각 $50\%\;32\%,\;13\%$와 $5\%$이었다. 푸른곰팡이병균은 감자배양액, 왁스만배양액과 리차드배양액에서 생육이 잘 되었으며 중성-염기성배지에서는 생육이 불량한 반면 산성에서 생육이 왕성하였으며 최적산도는 pH4였다. 양송이 수확기간 중 재배사내의 온도는 $15^{\circ}C$내외 일 때 본명의 발생이 적었고 수량이 많으며 $20^{\circ}C$이상에서는 본병의 발생이 격심하였다. 푸른곰팡이병균은 복토흙 소독시 $70^{\circ}C$에서 60분, 혹은 $80^{\circ}C$에서 30분간 열처리하므로서 완전히 사멸하였고 퇴비 후발효 과정에서도 사멸되었다. Four species of Trichoderma causing green mildew of Agaricus bisporus were isolated from 38spots of mushroom growing areas in Korea. These are T. koningi, T. lignorum, T. glaucum and an unidentified species, and their frequencies of occurrence are $50\%,\; 32\%,\; 13\%; and\; 5\%$ respectively. All of these species grew well in potato dextrose, Waksman's and Richard's solution, and preferred acid (pH. 4) to neutral. The temperature in mushroom trouse should be kept at $15^{\circ}C$ during cropping period, not only for the high yield of mushroom but also for the prevention of green mildew of mushroom caused by Trichoderma spp . T. lignorum was killed in soil on an exposure of 60 minutes at $70^{\circ}C$ and when exposed for 30minutes at $80^{\circ}C$. Peak heat procedure of compost eliminated T. lignorum and T koningi.
Won-Mok Son(손원목),Do-Yeon Kim(김도연),Ki-Dong Sung(성기동),Yi-Sub Kwak(곽이섭),Yeong-Ho Baek(백영호),Song-Young Park(박송영) 한국생명과학회 2015 생명과학회지 Vol.25 No.12
본 연구는 생후 3주령 Sprague-Dawley계 수컷 흰쥐를 16마리로 6주간 고지방식이를 통해 비만을 유도 후 운동군(8마리), 대조군(8마리)로 구분하였다. 운동기간 중 운동군과 대조군 모두 고지방식이를 섭취시켰다. 1주차는 14-15 m/min의 속도로 1일 30분, 2, 3주차는 15-16 m/min의 속도로 1일 35분, 4주차는 16-17 m/min의 속도로 1일 40분으로 주 6회 실시한 후 다음과 같은 결론을 얻었다. TC, TG는 운동군이 대조군 보다 유의하게 낮았으며, HDL-C는 운동군이 대조군 보다 유의하게 높았다. 혈전용해능, 산화질소는 운동군이 대조군 보다 유의하게 높았다. 이상을 종합하여 볼 때 유산소 운동이 혈관기능개선에 도움을 주는 것으로 나타났다. Although exercise training has been utilized to improve vascular function in animals and humans, the impact of moderate intensity exercise training on fibrinolytic activities and nitric oxide (NO) bioavailability has not been well documented. Therefore, the purpose of the current study was to examine the impact of moderate intensity aerobic exercise training on fat mass, blood lipid profiles, fibrinolytic activity, and NO levels in high-fat-diet induced rats. The body weight, fat mass, blood lipid profiles, fibrinolytic activity, and nitrite/nitrate were measured pre- and postexercise (10 weeks) training. The body weight and fat mass reduced significantly in the exercise (EX) group compared to the control (CON) group. Blood lipid profiles and low-density lipoprotein were unchanged in the EX group compared to the CON group. However, triglyceride and free fatty acid were significantly lower in the EX group compared to the CON group, and high-density lipoprotein was significantly greater in the EX group compared to the CON group. In addition, fibrinolytic activity and nitrite/nitrate were significantly greater in the EX compared to the CON group. These results suggest that 10 weeks of the moderated intensity aerobic exercise training improves blood lipid profiles, fibrinolytic activity, and the nitrite/nitrate ratio, which may improve vascular health and reduce obesity-related cardiovascular disease risks in high-fat- diet induced rats.
Effect of Circuit Training on Aging-related Hormones in Obese Middle-aged Women
Won-Mok Son(손원목),Yi-Sub Kwak(곽이섭),Gun-Do Kim(김군도),Min-Sung Ha(하민성),Song-Young Park(박송영),Gi-Dong Sung(성기동) 한국생명과학회 2017 생명과학회지 Vol.27 No.9
노화 과정에 따라 중년 여성들은 폐경 이후 체력이 저하되기 시작하면서 체지방 증가와 제지방량의 감소로 비만으로 이어지기 쉽고, 이러한 현상은 체내 호르몬의 분비의 변화와 함께 발생된다. 본 연구는 서킷 트레이닝이 비만 중년 여성의 노화 관련 호르몬에 미치는 영향을 구명하기 위해 실시하였다. 대상자 체지방률 30% 이상인 비만 중년 여성 운동군 10명, 대조군 10명 총 20명을 실시하였다. 운동 시간은 준비운동 5분, 본 운동 20분, 정리운동 5분으로 총 30분 주 3회 실시하였으며, 운동 강도는 운동자각도를 이용하여 13~17(약간 힘들다-매우 힘들다)수준으로 실시하였고, 또한 POLAR 심박수 변화량을 측정하여 1~6주는 60~70%HRR (Heart rate reserve), 7~12주는 70~80%HRR로 설정하였다. IGF-1은 운동군이 유의하게 증가하였으며, GH는 운동군이 유의하게 증가하였고, DHEA-S는 운동군이 유의하게 증가하였다. 이상의 결과 서킷 트레이닝이 비만 중년 여성의 노화 관련 호르몬에 긍정적인 영향을 준 것으로 사료된다. Due to aging, the physical fitness of middle-aged women decreases after menopause. This results in increased body fat and reduced lean body mass, both of which can lead to obesity. This phenomenon is accompanied by changes in hormone secretion in the body. The purpose of this study was to analyze the effects of circuit training on aging-related hormones in obese middle-aged women. The subjects were 20 obese middle-aged women. The subjects were divided into two groups: a circuit training group (n=10) and nonexercise control group (n=10). Growth hormone (GH), insulin-like growth factor-1 (IGF-1), estrogen, and DHEA-S were measured before and after the circuit training program. The circuit training group performed circuit training for 12 weeks, three times per week. Exercise intensity was increased gradually from 60% to 80% of heart rate reserve every 6 weeks. In the circuit training group, at the end of the 12-week training program, the IGF-1 levels were significantly increased compared to pre-exercise levels, and they were higher than those of the control group. GH levels were also significantly increased in the circuit training group, but the differences were not statistically significant. DHEA-S was significantly increased in the circuit training group, but the difference was not statistically significant. The results showed that circuit training improved aging-related hormones levels in obese middle-aged women.