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항정자 항체 검출을 위한 CIA 및 ELISA 개발을 위한 기초 연구
김세철,이기순,김윤규,김창규,최경호,권오중,김종배,Kim, S.C.,Lee, K.S.,Kim, Y.K.,Kim, C.K.,Choi, K.H.,Kwon, O.J.,Kim, J.B. 대한생식의학회 1990 Clinical and Experimental Reproductive Medicine Vol.17 No.1
항정자항체를 검사할 수 있는 면역분석법개발을 위하여 immunoaffinity chromatography로 분리 정제한 정자표현항원을 microtiter plate에 고정화 시킨것과 효소와 화학발광체로 표지된 2차항체를 사용하여 ELISA법과 CIA법을 개발하고 이 방법의 이용 가능성을 검토하기 위하여 기존 방법인 Kibrick test법으로 임상소견이 다른 남성혈청을 분석 비교하여 다음과 같은 결과를 얻었다. 1. 인간정자 표면항원을 분리하기 위한 immunoaffinity column을 제작키 위하여 인간정자를 토끼에 주사하여 정자에 대한 항혈청을 생산하였으며 이를 Protein A-Sepharose column으로 분리 정제하여 CNBr activated Sepharose-4B에 coupling시켜 immunoaffinity column을 제작하였다. 이 column에 균질화된 정자를 반응시키고 SDS를 넣은 Tris-HCI buffer로 용출시켰을때 60KD정도의 분자량을 갖는 분획을 얻었다. 2. 분리 정제된 human IgG를 microtier plate에 농도를 달리하여 고정화 시키고 ELISA용 Goat anti-human IgG-HRP conjugate와 CIA용 Rabbit anti-human IgG-ABEI-H conjugate와 반응시켜 그 활성도를 측정하였던 바 농도에 따라 반응의 정도가 감소하였다. 3. ELISA법으로 양성혈청의 희석곡선을 작성하였을때 경사가 완만한 것과 급한 것의 두 종류의 경향을 띈 곡선으로 대별되었으며 완만한 경사를 나타내는 것에서 1:160 희석치에서 O.D가가 0.1이하를 음성, 0.1이상 0.2이하를 약양성(weak positive) 그리고 0.2이상을 양성으로 판별하였다. 4. ELISA, CIA 그리고 Kibrick test로 동일시료를 분석 비교하였던바 ELISA와 CIA는 거의 동일한 상관관계를 보였으나 Kibrick test와는 50% 수준만 일치함을 보였다. New immunoassay systems for the detection of anti-sperm antibodies were developed. For this, sperm surface protein was purified by the immunoaffinity column prepared by the coupling of rabbit anti-human IgG antibodies to Sepharose-4B. Fraction eluted by tris-HCI buffer containing SDS showed a single band having molecular weight of about 60KD on electrophoresis. Enzyme HRP labelled goat anti-human IgG and chemiluminescence aminobutylethyl-isoluminol(ABEI) labelled rabbit anti-human IgG were used for ELISA and CIA, respectively. These two labelled conjugate bound well with human IgG. When serum dilution curves were made to titrate positive serums, two kinds of curves with steep and sluggish slopes were obtained Serum samples were categorized into 3 groups: positive, weak positive and negative based on slope of curve and O.D. values at 1:160 dilution of serum. When ELISA and CIA were compared to conventional method Kibrick test by the determinations of 62 male serums with different diagnosis, the results of ELISA and CIA agreed well, but both disagreed with that of Kibrick test. This study showed that purified sperm surface antigen can be used to develope solid-phase immunoassay systems such as ELISA and CIA which may eliminate the problems encounted the immobilization of living sperm in other tests.
김세철(Sae Chul Kim) 대한성학회 2020 대한성학회지 Vol.4 No.1
The prevalence of sexual dysfunction increases in older men due to aging and other medical diseases such as diabetes mellitus and hyperlipidemia. However, not all older men experience sexual dysfunction because the sexual life of each person is very diverse. Therefore, it needs to modify the legal retirement age of sex, reflecting the actual sexual life of older men. Thus, the author suggests a revised legal retirement age of sex Based on studies about sexual dysfunction in Korean men.
스테인리스강(ASTM Type 316L)과 탄소강(ASTM A516 Gr.70) 이종금속의 FCA 다층 용접부 특성에 대한 연구
김세철(Se Cheol Kim),현준혁(Jun Hyeok Hyun),신태우(Tae Woo Shin),고진현(Jin Hyun Koh) 대한용접·접합학회 2016 대한용접·접합학회지 Vol.34 No.3
Characteristics of dissimilar metal welds between ASTM Type 316L and carbon steel ASTM A516 Gr.70 made with FCAW were evaluated in terms of microstructure, ferrite content, EDS analysis, hardness, tensile strength, impact toughness and corrosion resistance. Three heat inputs of 10.4, 16.9, 23.4kJ/cm were employed to make joints of dissimilar metals with E309LMoT1-1 wire. Microstructure of dissimilar weld metals consisted of mostly vermicular type of δ-ferrite and some lathy type of δ-ferrite, and δ-ferrite was transformed into globular type in reheated zone. In all conditions, weld metals were solidified on FA solidification mode. Based on the EDS analysis of weld metals, All Creq/Nieq values were in the range of FA solidification mode, and it was decreased with increasing heat inputs whereas it was increased with increasing layers. The amount of δ-ferrite was decreased with increasing heat input due to the difference of cooling rate, and it was increased with increasing layers. Accordingly, hardness and tensile strength of dissimilar metals weld joints was decreased with increasing heat input while impact energy was increased with increasing heat input. Corrosion test of dissimilar metals weld joints showed that weight gain rate of heat input 10.4kJ/cm was the greatest, and that of three heat inputs became constant after certain time.
스테인리스강 STS 316L과 탄소강 A516-70의 이종금속 GTA 용접부 특성에 대한 연구
김세철(Se Cheol Kim),신태우(Tae Woo Shin),문인준(In Joon Moon),장복수(Bok Su Jang),고진현(Jin Hyun Koh) 대한용접·접합학회 2015 대한용접·접합학회지 Vol.33 No.4
Characteristics of dissimilar metal welds between STS 316L and carbon steel ASTM A516 Gr.70 made with GTAW have been evaluated in terms of microstructure, ferrite content, chemical analysis, hardness and corrosion resistance. Three heat inputs of 9.00, 11.25, 13.00kJ/cm were employed to make joints of dissimilar metals with ER309 wire. Based on microstructural examination, the amount of vermicular type of δ-ferrite was increased with increasing heat input due to the increase of Creq /Nieq in the second layer of welds. Based on the EDX analysis of weld metals, Cr and Ni content in the 2nd layer increased while those content in the first layer of welds decreased with heat inputs. Cellular solidification mode in the 1st layer and dendritic solidification mode in the 2nd layer due to different cooling rates were prevailed, respectively. Heat affected zone which formed hard microstructure showed higher hardness than the weld metal. The salt spray test of dissimilar metals weld joints showed that the carbon steel surfaces only corroded. The weight loss rate due to corrosion increased up to 100hours but it decreased above 100 hours. There was little difference in the weight loss caused by corrosion regardless of heat inputs.