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2분과 : 밀원수종 쉬나무 수꽃과 암꽃의 화밀분비량, 당 함량 및 아미노산 분석
김문섭 ( Moon Sup Kim ),김세현 ( Sea Hyun Kim ),송정호 ( Jeong Ho Song ),김혜수 ( Hyeu Soo Kim ) 한국임학회 2014 한국산림과학회지 Vol.103 No.1
본 연구는 밀원수종으로 관심의 대상이 되고 있는 쉬나무를 대상으로 수꽃과 암꽃의 화밀 분비량, 당 함량및 아미노산을 분석하고 수확할 수 있는 꿀의 양을 추정하기 위하여 실시하였다. 경기도 화성시의 2012년 쉬나무 수꽃의 개화 최성기는 7월 24일부터 26일이었으며, 평균 화밀분비량은 2.73±0.73 μL, 화밀 당도는 17.4%를 나타냈다. 2012년 암꽃의 개화 최성기는 8월 7일부터 9일이었으며 평균 화밀분비량은 0.63±0.49 μL, 화밀 당도는 25.7%를 나타냈다. 기상인자와 상관분석 결과, 화밀 분비량과 화밀 당도는 온도와 습도의 영향을 많이 받는다는 것을 알 수 있었다. 반면, 꽃 한 개당 당 함량을 산출한 결과, 수꽃은 48.0±5.2 μg, 암꽃은 37.8±8.7 μg으로 분석되었으나, 유의적인 차이는 없었다(Mann-Whitney’s U-test, p=0.400). 이러한 당 함량을 가지고 수꽃 1개 화서에서는 최소 67.8 g, 암꽃 1개 화서에서는 최소 53.3 g의 꿀을 수확할 수 있을 것으로 추정하였다. 또한 아미노산 분석 결과, Serine, Glycine및 Alanine은 수꽃에서 더 많은 함량이 검출된 반면, Asparatate, Glutamate, Asparagine 및 Glutamine은 암꽃에서 더많은 함량을 나타내어 수꽃과 암꽃에서 7개의 아미노산 함량이 유의적으로 차이가 있음을 알 수 있었다. The purpose of study was to analyze secreted nectar volume, nectar sugar content and amino acid in addition to estimating honey quantities that can ultimately reap in male and female flowers of Evodia danielliiHemsl.. The maximum blooming period of male flowers was on 24 to 26 July in 2012. On average, nectarvolume secreted by nectary was 2.73±0.73 μL from one male flower and nectar concentration showed 17.4%. The maximum blooming period of female flowers was on 7 to 9 August in 2012. Nectar volume secreted bynectary was 0.63±0.49 μL from one female flower and nectar concentration showed 25.7%, averagely. As results of correlation analysis between the meteorological factors and nectar characteristics, we found that nectar quantities and concentration were influenced by temperature and relative humidity. Sugar content was calculated at 48.0±5.2 μg per a male flower and 37.8±8.7 μg per a female flower, which meant that both values were not significantly different (Mann-Whitney`s U-test, p=0.400). The minimum estimates of honey harvest for a male and female inflorescence were 67.8 g and 53.5 g, respectively. Analysis of amino acid showed that Serine, Glycine and Alanine were more abundant in male flowers, however Asparatate, Glutamate, Asparagine and Glutamine were more abundant in female flowers.
김문섭(Moon-Sup Kim),김세현(Sea-Hyun Kim),한진규(Jin-Gyu Han),송정호(Jeong-Ho Song),김혜수(Hyeu-Soo Kim),김도훈(Do-Hoon Kim),권용삼(Yong-Sham Kwon) 한국육종학회 2015 한국육종학회지 Vol.47 No.1
The objective of this study was carried out to assess genetic diversity of Korean local accessions of wild pear using morphological traits and microsatellite markers. Assessment of 14 phenotypic traits showed high variation among wild pear accessions. These parameters were not applicable genetic diversity analysis of wild pear collection due to quantitatively inherit and their expression is affected by environmental factors. Microsatellite markers were used to evaluate the genetic similarity of 62 accessions. Among 50 tested SSR markers, 16 primer pairs were selected to profile genetic diversity on the basis of high level polymorphism. These microsatellite markers showed 1 or 2 discrete amplified fragment for all of the accessions. The sixteen microsatellite loci amplified 278 alleles, with 10 to 27 alleles (average 17.385) per locus. The mean of observed heterozygosity and polymorphism information content were 0.776 and 0.836, respectively. The Jaccard’s similarity coefficient ranged from 0.09 to 1.00. Two major groups were produced from all the accessions by UPGMA cluster analysis. Fifty five accessions could be discriminated except for 2 pairs. These results would be provided useful information about valuable genetic resources though assessment of genetic diversity and relationship in local pear accessions.