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Infectious bursal disease virus 국내분리주 및 백신주의 VP2 gene의 비교분석
김길동,강정무,김선중,권혁무,한태욱,Jin, Ji-Dong,Kang, Zheng-Wu,Kim, Sun-Joong,Kwon, Hyuk-Moo,Hahn, Tae-Wook 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.3
The VP2 full gene of Korean infectious bursal disease virus(IBDV) strain, SH/92, three attenuated vaccine strains, Bur706, Bursine-2 and CEV/AC strains, were amplified by reverse transcriptase-polymerase chain reaction and sequenced and compared with published VP2 gene sequences of IBDVs. The VP2 nucleotide sequence similarity between SH/92 and three vaccine stains was 95.6~96.5% whereas the nucleic acid similarity among three vaccine strains was 97.5~98.5%. The amino acid sequence similarity of VP2 of SH/92 compared with three vaccine strains was between 94.4 and 97.6% while the amino acid similarity among three vaccine strains was between 97.4 and 98.4%. The amino acid similarity between SH/92 and classical virulent strain, 52/70 and STC strain was 96.4 and 96.5%, respectively. The serine-rich heptapeptide was conserved in CEVAC and Bursine-2 as well as SH/92 but not in Bur706. The phylogenetic tree developed from amino acid sequences showed that SH/92 was categorized with vv IBDVs(HK46, OKYM, KKI, UPM94/273, SH95) in one branch while three vaccine strains were catagorized with STC strain in the other branch.
닭 Mycoplasma gallisepticum 6/85 생균 백신의 효능 평가
윤희준,강정무,김길동,신은경,정용운,정지혜,한태욱,Yoon, Hee-Jun,Kang, Zheng-Wu,Jin, Ji-Dong,Shin, Eun-Kyung,Jeong, Yong-Hoon,Jeong, Ji-Hye,Hahn, Tae-Wook 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.3
Mycoplasma gallisepticum (MG) continues to persist in many commercial layer farms in Korea,resulting in losses in egg production. Bacterins and live attenuated vaccines have been used for the prevention of losses caused by MG. One of these attenuated vaccines, MG 6/85 vaccine has been reported to be safe and efficacious in layers. However, MG 6/85 vaccine has not been evaluated for its safety and its efficacy in any commercial layer in Korea. Six-week-old specific pathogen-free (SPF) chickens were vaccinated with MG 6/85 vaccine by aerosol and were challenged with virulent MG R strain at 4 weeks after vaccination. The vaccinated group was able to resist challenge into the air sacs because the vaccinated group showed much less air sac lesion compared with the unvaccinated group. Each of two commercial layer farms was divided into vaccinated and unvaccinated groups. For each vaccinated gorup, MG 6/85 vaccine were sprayed at 17 week old on farm A and at 15 weeks old on farm B. Hen-day egg production, Hen-housed eggs, egg weight, mortality were evaluated until 50 week after vaccination.Compared with the unvaccinated group in each farm, the vaccinated group showed higher average egg production and egg weight, and higher hen-housed number. Results of this study are in agreement with other previous reports which demonstrated that MG 6/85 vaccine favorable effect on performance in commercial layers.
비구조 단백질 유전자 primer를 사용한 RT-PCR에 의한 인플루엔자 A형 바이러스의 검출
문형선 ( Hyeong Sun Moon ),배윤영 ( Yoon Yeong Bae ),김길동 ( Ji Dong Jin ),강정무 ( Zheng Wu Kang ),한태욱 ( Tae Wook Hahn ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.2
Influeza type A virus have been worldwide problematic in animals as well as in humans. In this study, the use of reverse-transcriptase polymerase chain reaction (RT-PCR) was described for detecting influenza virus type A. The primer of RT-PCR was designed from an nonstructural (NS) gene of Influenza A virus. By RT-PCR, a product with the size of 189 bp was detected only when influenza virus type A was used as template. No products could be detected with Influenza virus type B as well as other respiratory pathogens. The detection limit of the RT-PCR was up to 100.3TCID50 which is comparable to the sensitivity of cell culture method. The RT-PCR could detect the influenza A virus from nasal turbinates of the ferrets infected with influenza virus type A not type B.