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해산 녹조료 참홑파래 , Monostroma nitidum 의 원형질체 분리와 분화
조용철(Yong Chul Cho),공용근(Yong Gun Gong),윤장택(Jang Taek Yoon),선상미(Sang Mi Sun),정규화(Gyu Hwa Chung) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.1
High yields of protoplasts were obtained following enzymatic digestion of the vegetative thalli of marine green alga Monostroma nitidum. The enzyme mixtures containing 4% Cellulase R-10+3% Macerozyme R-10+3% Abalone acetone powder produced 4.41×10^6 protoplasts per 300 ㎎ of fresh tissue. The highest yield of protoplasts was obtained by 270 minutes treatment of the thalli in enzyme solution. Freshly isolated protoplasts were spherical in shape and ranged between 13∼33 ㎛ in diameter. The high efficiency of differentiation were obtained by incubating freshly isolated protoplasts in 0.4 M mannitol f/2 medium for 7 days and then transferring to 0.2 M mannitol f/2 medium. Protoplasts began to form new cell walls three days after initial culture and began to germinate after 10 days, and then form a leafy thallus after further culture in f/2 medium. The addition of antibiotics in media inhibited the differentiation of protoplasts in culture.