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강경신(Kyung Shin Kang),이승재(Seung-Jae Lee),조동우(Dong-Woo Cho) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.11
The purpose of this study was to examine the effects of various mechanical stimuli for MC3T3-E1 cells. Among the several mechanical stimulations, we focused on compressive stain and ultrasound. In this study, we developed a bioreactor capable of applying controlled stimuli to scaffolds. PLLA/PCL scaffold was fabricated by using salt-leaching method. We performed dynamic cell culture using preosteoblasts MC3T3-E1 cells with 1MHz, 30㎽/㎝2 ultrasound and 10% of compressive strain. Result of CCK-8 analysis at 1, 4, 7, 10 days showed that mechanical stimuli had no significant effect for cell proliferation. However, those stimuli influenced ALP(Alkaline phopatase) activity, which is one of differentiation marker.
전자기장 자극이 3차원 알지네이트 젤 구조물을 이용한 연골 조직 재생에 미치는 영향
이희경(Hee-Gyeong Yi),강경신(Kyung Shin Kang),홍정민(Jung Min Hong),장진아(Jinah Jang),조동우(Dong-Woo Cho) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
For the cartilage regeneration, the study of cell-based tissue engineering introduced a three-dimensional hydrogel construct encapsulating chondrogenic cells or stem cells. In order to enhance the regeneration ability, we observed the effect of an electromagnetic field (EMF) on the behaviors of chondrocytes in the 3-dimensional hydrogel. Human chondrocytes, which is embedded into the alginate gels, were exposed to the EMF at two kinds of frequencies (7.5 and 45 Hz). The 7.5 Hz-EMF did not show a significant effect on chondrocytes than that of the control, while the 45 Hz-EMF stimulated glycosaminoglycan (GAG) synthesis and the expressions of chondrogenic markers such as SOX 9, ACAN, and COL 2. Thus, the 45 Hz EMF-stimulation was helpful tool for accelerating cartilage tissue regeneration.
골조직 재생을 위한 자유형상제작 기반 BMP-2 서방출형 HA-PLGA 인공지지체 개발
심진형(Jin-Hyung Shim),박정규(Jung Kyu Park),김종영(Jong Young Kim),강경신(Kyung Shin Kang),한세광(Sei Kwang Hahn),조동우(Dong-Woo Cho) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
Solid freeform fabrication (SFF) based three dimensional scaffolds for bone regeneration were successfully fabricated with blend of poly(lactic-co-glycolic acid) grafted hyaluronic acid (HA-PLGA) and polyethyleneglycol (PEG) encapsulating intact BMP-2. HA-PLGA was synthesized by the conjugation between adipic acid modified HA (HA-ADH) and PLGA. PEG was blended with HA-PLGA to encapsulate BMP-2 in chloroform without denaturation, which were exploited to fabricate tissue engineered scaffolds. Multi-head deposition system, a SFF technology, was used to fabricate 3D scaffolds. In-vitro release tests confirmed the sustained release of intact BMP-2 from the scaffolds up to a month. After the in-vitro assessments of proliferation and differentiation, the blended HA-PLGA/PEG/BMP-2 scaffolds were implanted into the calvarial bone defects in SD rats. Micro-CT and histological analyses revealed effects of HA-PLGA/PEG/BMP-2 scaffolds on bone regeneration.