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Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석
허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1
B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고, 그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다 1. B. abortus 분리주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행 한 바 공시한 참조균주와 분리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.6%~l00% 및99.5%~99.9%의 매우 높은 상품성을 나타내었고, B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상품성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바, 분리균주들은 B. abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%~l00%의 매우 높은 상품성을 보였으며, B. abortus 45/20 (biovar 1)과는 96.4%~97.2%의 상품성을 보였다. 3. 본 시험에 공시된 브루셀라균주의 omp2b 유전자서열과 여러 동물에서 분리된 브루셀라 분리주의자료를 바탕으로 phylogenetic tree를 작성 한 바, 국내 분리주는 B. abortus 2308 (biovar 1), B. abortus B3196 (blovar 5), B. abortus 45/20 (biovar 1), B. suis 1330 (biovar 1), B. melitensis 16M (biovar 1), B. canis NCTC 01854, B. neotomae 5k33과 97.3%이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며, omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OW) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa OMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%-100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.
Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석
허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8
B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기 위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고,그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다. 1. B. abortus 분리 주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행한 바 공시한 참조균주와 분 리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다. 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리 균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B abortus B3196 (biovar 5)과는 99.6%∼100% 및 99.5%∼99.9%의 매우 높은 상동성을 나타내었고,B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상동성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바,분리균주들은 B.abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%∼100%의 매우 높은 상동성을 보였으며,B. abortus 45/20 (biovar 1)과는 96.4%∼ 97.2%의 상동성을 보였다. 3. 본 시험에 공사된 브루셈라균주의 omp2b 유전자 서열과 여러 동물에서 분리된 브루셀라 분리주의 자료를 바탕으로 phylogenetic tree를 작성 한 바,국내 분리주는 B. abortus 2308 (biovar 1),B. abortus B3196 (biovar 5),B. abortus 45/20 (biovar 1),B. suis 1330 (biovar 1),B. melitensis 16M (biovar 1),B. canis NCTC 01854,B. neotomae 5K33과 97.3% 이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며,omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OMP) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa aMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%∼100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.
허태철,주성현 慶北大學校 農業科學技術硏究所 2004 慶北大農學誌 Vol.22 No.-
This study was conducted to provide a fundamental information for commercial, medical usage and mushroom gene preservation and to investigate the diversity of indigenous mushroom on Bonghwa-Gun. A total of 3 classes 2 subclasses 13 orders 45 families 64 genera and 118 species including saprophytic and ectomycorrhizal fungi was investigated. The mushroooms are classified into 17 families 32 genera and 80 species in Agaricales, 13 families 17 genera and 22 species in Aphllophorales, 2 families 2 genera and 2 species in Heterobasidiomycetes, 9 families 9 genera and 10 species inGasteromycetidae. There were edible mushroom of 46 species at Moonsu and Chungryang Mountain. The mushroom occurrence was closely related to vegetation and soil environmental conditions.
허태철,주성현 慶北大學校農業科學技術硏究所 2002 慶北大農學誌 Vol.20 No.-
This study was carried out in order to produce useful material for the forest multiple use and forest protection by soil physico-chemical analysis of studied area in Mt. Palgong. The results of soil physico-chemical analysis and statistical analysis represented as following 2 points. 1. Soil depth was in the range of average 61.1 cm and soil texture was loamy sand and sandy loam except Donghwasa area. The part of solid phase and gaseous phase were higher than other areas, but liquid phase was less in verse. Soil water content was in an average 49.5%, penetrability was average 1.95×E^2cm/sec and the average of soil hardness was 1.64 Kg/㎠. This data showed that soil water content, penetrability and soil hardness were good at Mt. Palgong forest soils. 2. Soil pH was the range of 3.4 to 6.0, organic carbon content was 2.8% that is nearly mean of the Korea brown forest soils, total N content is somewhat smaller than that of other places, and total average C/N ratios was 13.9. Average available P_2O_5 concentration was 5.05 mg/kg that is lower than that of any others. The concentration of available P of coniferous forests is higher than that of deciduous forests. Exchangeable cations content is similar to those of the Korea brown forest soil and the order of the cation content extent is Ca^(2+) > Mg^(2+) > Na^+ > K^+.
울진 소광리 금강소나무림의 송이발생지와 능이발생지의 토양환경 비교
허태철,주성현 慶北大學校農業科學技術硏究所 2002 慶北大農學誌 Vol.20 No.-
This study was carried out in order to produce useful material for the forest multiple use and forest protection by physico-chemical soil analysis of studied area in Sokwang-ri Forest Genetic Resource Protection Forest which was divided into in standard plots include Tricholoma matsutake and Sarcodon aspratus production forest. The result of physico-chemical soil analysis represented as following. The soil type of T. matsutake production forest was Dry brown forest soil(B1), while on the other hand the soil type of S. aspratus production forest was Moderately moist brown forest soil(B3). Between T. matsutake and S. aspratus production forest did not result in significant changes in soil pH(5.22-5.60) and soil depth(47cm), but available phosphorus, carbon, and nitrogen contents were different results. CN ratio of the fairy ring of T. matsutake was quite lower than in S. aspratus production forests, which indicated that T. matsutake production forest was built up in the relatively immature soils which contain little organic matter. Generally, it was predicted that Pinus densiflora for. erecta forest succeeded to deciduous tree forest in stable soil environments. To conserve these T. matsutake and S. aspratus production forest, the contents of available phosphorous and exchangeable cation should be increased by continuous soil environment management and it should be established the secondary growth forests of old aged Pinus densiflora for erecta trees as soon as possible.
허태철,주성현 慶北大學校 農業科學技術硏究所 2005 慶北大農學誌 Vol.23 No.-
This study was carried out in order to produce useful material for the forest multiple use and forest protection management by soil physio-chemical analysis of studied area in Mt. Namsan. The result of soil physio-chemical analysis and statical analysis represented as following. In side of physical properties of forest soil in Mt. Namsan, Soil depth was average 31.4cm and available soil depth was average 20.0cm. Soil type was Brown forest soil that representative soil type in Korea. Soil texture was sandy loam(SL) except valley area. In side of chemical properties, the range of soil acidity was 4.29~5.19 (average 4.76), organic matter content was 3.17% that compared the lowest value to organic matter content of Korea forest soil. Available phosphorus was 3.64ppm that was lower than others forest soil. Exchangeable cation content was similar to the Korea brown forest soil. Cation exchange capacity(CEC) was 8.22 cmol_(c)/kg in Mt. Namsan.