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      • KCI등재

        자연산 점몰개, Squalidus multimaculatus Hosoya et Jeon의 새인두흡충, Clinostomum complanatum (Rudolphi, 1814) 감염

        박명애 ( Myoung Ae Park ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),최희정 ( Hee Jung Choi ),전은지 ( Eun Ji Jeon ),지보영 ( Bo Young Jee ),이완옥 ( Wan-ok Lee ),우승호 ( Sung Ho Woo ),이은혜 ( Eun Hye Lee ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2

        경상북도 영덕군의 하천에 서식하는 점몰개 spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon)의 근육에서 새인두흡충 Clinostomum complanatum (Rudolphi, 1814)의 피낭유충이 발견되었다. 감염된 점몰개는 "yellow grub"인 기생충의 피낭유충으로 인해 표면이 울퉁불퉁하게 보였으며, 수면위에 힘없이 유영하였고 일부 폐사한 개체도 발견되었다. 분리된 탈낭유충의 형태는 혓바닥 모양이었으며, 크기는 2.85~5.89 mm×0.8~1.99 mm였다. 어체당 1~98 개의 피낭유충을 분리하였으며 평균 감염수는 25개로 나타났다. 특히, 성어크기인 평균 7 cm 정도의 점몰개는 100% 감염되어 있었다. 본 연구는 한국의 자연수계에 서식하는 어류에서 C. complanatum 의해 질병이 발생하고 폐사가 일어난 첫 보고이다. The metacercariae of Clinostomum complanatum (Rudolphi, 1814) were isolated in the whole body muscle of wild freshwater fish, spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon) from the local river in Yeongdeok, Gyeongsangbuk-do, Korea. The infected fish with bumpy body surface by "yellow grub" showed erratic swimming behavior and some fish were died. The isolated excysted metacercariae were tongue shaped and 2.85~5.89 mm × 0.8~1.99 mm in size. 1~98 metacercariae were isolated in individual fish and mean infection intensity was 25. All examined adult spotted barbel gudgeon sizing 7 cm in body length were infected. This is the first report of disease outbreak and mortality caused by C. complanatum infection in wild freshwater fish in Korea.

      • KCI등재
      • 학동기 자녀를 둔 어머니의 아동실에 대한 의식 : 시설복지적 측면에서 본 아동공간에 관한 연구(Ⅲ) A Study on childrens Space from a Welfare Viewpoint (Ⅲ)

        안옥희,박인전 영남대학교 지역발전연구소 1994 새마을지역개발연구 Vol.16 No.-

        This study was designed with the purposes to translate the parent's consciousness of children's room and to produce the basic data for the guidance and the effective preparation of children's room and the children s residing environment in addition, by grasping generally the mother's consciousness of children's room. The subjects were 212 4th-6th grade elementary school children living in Taegu. The data were collected by questionnaire methods and analyzed through SPSS PC program. The major findings are as follows : 1. The greater part of mothers recognized that the children's room is necessarily needed to children. 66 74 among all subjects perceived that the adequate period of sleeping separation from their parents is before the entrance of elementary school. It was found that in the children s hope for the possession of their room from mother's viewpoint, the majority of mothers perceived that their children's very wanted to have their own room. The greater part of mothers perceived that the environmental preparation of children's room have an effect on their personality. And when mothers decide the location of children s room they regarded the natural environment such as sunlight and airing as the most important matter. 2. There were significant differences in the mother's consciousness of the necessity of children's room according to the independent variables such as the conditions of housing possession, mother's educational level. The type of using of children s bedroom was variables according to family numbers, the order of birth, housing style, the numbers of room, environment around residing. The adequate periods of sleeping separation from their parents were significant different in the variables as children's sex, father and mother's educational level. There were significant differences in the consideration for the interior of children's room according to father's occupation, father and mother's educational level. There were significant differences in the effect of the environmental preparation of children's room on children's personality according to only father s occupation.

      • 말더듬 아동과 일반 아동의 유창성 특성 비교

        고영옥,전희숙,권도하 대구대학교 특수교육재활과학연구소 2007 再活科學硏究 Vol.25 No.2

        The purpose of this study was to investigate characteristics of stutter children on the speech task. The speech task was automatic speech, reading, and monologue. we collect speech sampling during of task. In the study participated seven stuttering children of 7 to 12years old(4 males, 3 females) and seven nonstuttering children(5 males, 2 females). The results of this study were as following. First, there was not significant difference between stuttering children and nonstuttering in the articulation rate. On the automatic speech task, stuttering children articulation rate was mean 171.60(SPM) and nonstuttering children articulation rate was mean 168.34(SPM). On the reading task, stuttering children articulation rate was mean 225.82(SPM) and nonstuttering children articulation rate was mean 218.09(SPM). On the monologue task, stuttering children articulation rate was mean 195.58(SPM) and nonstuttering children articulation rate was mean 191.95(SPM). Second, In the stutter children, dysfluency syllable number varied on the speech task. Also, stutter children, dysfluency syllable rate varied on the speech task. On the automatic, monologue task, dysfluency syllable number was mean 8.57syllable (SD=9.71), 20.71syllable (SD=13.47). On the automatic, monologue task, dysfluency syllable rate was mean 15.85%(SD=32.98), 29.00%(SD=19.74). 본 연구의 목적은 말더듬 아동이 각기 다른 구어 과업에서 유창하게 말할 때의 구어 특성을 알아보는 것이었다. 본 연구의 대상은 7~12세의 말더듬 아동 7명(남자 4명, 여자 3명)과 일반 아동7명(남자 5명, 여자 2명)이었다. 자동구어와 독백 과업을 실시하는 동안 구어샘플 수집하였다. 연구의 결과는 다음과 같다. 첫째, 말더듬 아동과 일반 아동의 유창한 구어의 속도에는 차이는 없었다. 자동구어 및 독백 과업 모두 유의한 차이가 없었다. 둘째, 과업별 말더듬 아동의 비유창한 음절수는 구어과업에 따라 다양하게 나타났다. 그리고 비유창한 음절 비율도 구어과업에 따라 다양하게 나타났다. 자동구어, 읽기, 독백과업에서 말더듬 아동의 비유창한 음절수는 평균 8.57음절(SD=9.71), 27.57음절(SD=23.47), 20.71음절(SD=13.47)이었다. 비유창한 음절비율은 자동구어, 독백과업에서 평균 15.85%(SD=32.98), 61.35%(SD=106.08), 29.00%(SD=19.74)이었다.

      • 초등학교 아동의 방과후 생활실태

        석주영,안옥희,박인전 영남대학교 지역발전연구소 1998 새마을지역개발연구 Vol.22 No.-

        This study aimed to investigate the actual conditions of after-school life, and to explore the actual conditions of, and perceptions of, children's playing time. The subjects for this study were 866 children of the 2nd, 4th, and 6th grades living in the Taegu Metropolitan Area. The data was analyzed by the four different methods, which were frequency, percentile, chi-square test, and one-way ANOVA using the 7.5 version of SPSSWIN program. The major results of this study were a s follows : First, children's after-school playtime decreased as their grades increased. Second, the percentage of children who perceive their after school playing time as being insufficient was the highest. The main reason children gave for having a lack of after school playing time was that they go to the private institutes.

      • 아동보육 및 교육관련시설에 대한 어머니의 만족도

        안지연,안옥희,박인전 영남대학교 지역발전연구소 1998 새마을지역개발연구 Vol.23 No.-

        The purpose of this study was to investigate mother's contentment degree of the institutions related to child care and education. The subjects were 463 mothers with children enrolled in the institutions related to child care and education, who lived in T city. The frequency, percentile, means, x^(2) test, ANOVA and Scheffe´-test were used for data - analysis. The major findings showed that (1) mothers with children enrolles in the institutions related to child care and education more importantly perceived the distance to home, regular and consistency education, sociality development than factors when they choose a institution. (2) the degree of satisfaction of mothers with children enrolled in the institutions related to child care and education were significant differences in the teaching tools, facilities and equipments. (3) the degree of satisfaction of mothers with children enrolled in the institutions related to child care and education were no significant differences in the teacher's quality, relationship to teachers. (4) the degree of satisfaction of mothers with children enrolled in the institutions related to child care and education were significant differences in the general management(hours of operation, cost, etc.). (5) the degree of satisfaction of mothers with children enrolled in the institutions related to child care and education were significant differences in the nutrition, safety, care.

      • 전남지역 학교급식의 위생관리 실태

        고무석,정난희,이전옥 한국가정과학회 2004 한국가정과학회지 Vol.7 No.1

        This study analyzed the effects of nutrition technicians' hygiene education on cooking workers' performance of hygiene management in order to ensure the security of school meals. The situation of cooking workers' disposition in subject schools was elementary school(51.1%) and middle school(48.9%) and the type of meals was rural area type(54.2%), urban type(36.5%), and island and isolated area type(9.3%). The methods of meals management were single cooking(88.2%) and joint cooking and management(11.8%). The type of distributing meals was distributing in a dining room(93.5%), in a classroom(3.7%), and in both dining room and classroom(2.8%). Nutrition technicians' employment form included regular(53.5%) and daily(88.2%). Their education was junior college graduate(50.2%), university graduate(44.8%), and graduate school students(5.0%). Cooking workers' employment form included daily(88.2%) and regular(11.1%), suggesting that most were regular. Most cooking workers(77.4%) had at least high school certificate. Regarding the situation of cooking workers' disposition in subject schools, the number of student per one cooking worker was found as 91-120(37.2%), 61-90(22.6%), 60 and under(21.1%), 121-150(16.7%), and 151 and over(2.5%). Cooking workers' level of performance of hygiene management was post-working stage(66.37/75 marks), pre-working stage(64.22/75 marks), and working stage(20.34/25 marks). The counting of meals articles in a pre-working stage(20.34/25 marks), temperature and required time in a working stage(18.78/25 marks), and machinery equipment and hygiene in a pre-working stage(21.40/25 marks) showed lowest of performance, which suggest poor service of hygiene. Cooking workers' performance of hygiene management by working stage showed the significant difference with school class(p<.001), type of schools with meals(p<.05), state of cooking workers' employment(p<.001), and cooking worker's disposition(p<.05). A working stage showed the significant difference with type of schools with meals(p<.05). A post-working stage showed the significant difference according to type of schools with meals(p<.05), and the methods of meals management(p<.05), and cooking workers' disposition(p<.05). In the execution of hygiene education, individual hygiene was highest(94.8%), followed by the management of machinery equipment and tools(89.7%), food poisoning and microorganism(94.7%), and the method of food treatment(76.4%). A yearly plan of hygiene education included established(83.9%) and not established(16.1%). Regular education included not executed(25.1%), 2~3 times a month(16.1%), and more than 4 a month(4.0%) and occasional education was not executed(57.0%), 1-3 times a month(26.3%), and more than 4 a month(15.7%). In the methods for hygiene education, oral education(95.7%) was used most, followed by demonstration(10.5%), poster/photo(10.5%), video/slide(3.7%), and computer(3.7%). Frequency of improvement and complement of hygiene education included once a month(56.3%), once a year(20.7%), by quarter(11.5%), and every six months(11.5%). Newspaper was used most in materials of hygiene education, followed by internet, TV, nutrition technician's reeducation, information exchange between members, educational office's training, and reference book, and educational office's material, and symposium. Cooking workers' assessment of the effect of hygiene education was conducted through observation(56.8%), check table(15.2%), question(14.0%), and examination(14.0%). The reason of cooking workers' low level of performance included habitual custom(53.9%), lack of understanding(20.4%), overwork(14.6%), and lack of knowledge(11.1%) and the reason of difficulty in hygiene education included lack of time(55.3%), lack of understanding(27.6%), lack of knowledge and information(8.7%), and lack of budget(48.0%).

      • An Anticoagulant/Fibrinolytic Protease from Lumbricus rubellus

        Jeon, Ok-Hee,Moon, Woong-Joon,Kim, Doo-Sik Korean Society for Biochemistry and Molecular Biol 1995 Journal of biochemistry and molecular biology Vol.28 No.2

        An anticoagulant/fibrinolytic protease was purified to homogeneity from the earthworm Lumbricus rubellus. The protein was a single chain glycoprotein of 32 kDa that exhibited strong proteolytic activity on human thrombin and fibrin clots. Proteolytic degradation of these plasma proteins by the purified enzyme occurred at a neutral pH range. Among several human plasma proteins tested as possible substrates for the protease reaction, the 32 kDa enzyme specifically hydrolyzed both thrombin and fibrin polymers without affecting other proteins, such as serum albumin, immunoglobulin, and hemoglobin. Treatment of the purified enzyme at neutral pH with either phenylmethylsulfonylfluoride or soybean trypsin inhibitor resulted in a loss of catalytic activity. The enzyme hydrolyzed the chromogenic substrate H-D-Phe-L-Pipecolyl-L-Arg-p-nitroanilide with a $K_m$ value of 1.1 ${\mu}M$ at a neutral pH. These results suggest that the anticoagulant/fibrinolytic enzyme from Lumbricus rubellus is a member of the serine protease family having a trypsin-like active site, and one of the potential clevage sites for the enzyme is the carbonyl side of arginine residues in polypeptide chains.

      • A Refolding Strategy for Recombinant Metalloprotease

        Jeon, Ok-Hee,Kim, Doo-Sik Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.3

        The partial cDNA of the MT-c clone encoding snake venom metalloprotease was subcloned and expressed in E. coli. The expressed metalloprotease was purified by affinity chromatography in the presence of urea, and then successfully refolded into its functional form, retaining metalloprotease activity that hydrolyzes fibrinogen. The simple and convenient refolding strategy established in this work was highly efficient in recovering the recombinant enzyme activity. Experimental evidence suggests that the C-terminal amino acid stretch of 16 residues is a critical sequence for proper folding of the metalloprotease domain.

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