http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
The development of practice model for uterus auto-transplantation using domestic pigs
( Jae Young Pae ),( Arvind Manikantan Padma ),( Mats Hellstrom ),( Mihai Oltean ),( Mats Brannstrom ),( Min Jong Song ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-
Objective: The present study aims to develop a practice model for uterus auto-transplantation using domestic pigs. Methods: Ten domestic pigs were used to develop a practice model. First 3 pigs are used for anatomical investigation to choose proper vessels used for anastomoses. Seven pigs were used to perform the uterus auto-transplantation. Under general anesthesia, the bicornuate uterus was carefully dissected and harvested with its bilateral feeding arteries and draining veins. The feeding arteries were bilateral umbilical arteries and the draining veins were bilateral ovarian arteries. After harvesting the uterus, bilateral arteries were cannulated immediately and the uterus was flushed with cold (+4 C) Custodiol (HTK) solution supplemented with xylocaine (0.4 mg/ml). The uterus was stored at +4 cooling chamber during during flushing. Flushed uterus was placed in its original position and the bilateral umbilical arteries and bilateral ovarian veins were anastomosed end to side to bilateral external iliac arteries and veins respectively. Indocyanine green (ICG) angiography was performed to monitor of reperfusion for 1hour. Results: Mean total operation time was 298 minutes and mean duration of donor surgery was 119 minutes. The mean cold ischemic time was 56 minutes and the mean warm ischemic time was 123 minutes. Estimated mean blood loss was 200 mL. All seven auto-transplanted pigs were considered well isolated and flushed. They also showed satisfactory reperfusion judged by change in gross appearance and ICG angiography. Conclusion: This study showed that a practice model for uterus auto-transplantation using domestic pigs is feasible and demonstrated that a satisfactory reperfusion could be achieved.
( Yoojin Na ),( Arvind M Padma ),( Jae Young Pae ),( Tagrid Jar-allah ),( Mats Brannstrom ),( Mats Hellstrom ),( Min Jong Song ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-
Objective: The present study aimed to develop an ex vivo sheep uterus reperfusion platform that mimics the reperfusion situation so that initial assessments and comparisons can be performed without the need for costly and labor-intensive in vivo transplantation experiments. Methods: Isolated sheep uteri were perfused with the preservation solution IGL-1 and were then exposed to cold ischemia for either 4 (n = 6) or 48 hours (n = 7). Uteri were then reperfused for 48 hours under normothermic conditions with an oxygenated recirculating perfusate containing growth factors and synthetic oxygen carriers. Histological and biochemical analysis of the perfusate was conducted to assess reperfusion injury. Results: Quantification of cell density indicated no significant edema in the myometrium or in the endometrium of uteri exposed to 4 hours cold ischemia and then a normothermic ex vivo reperfusion for 48 hours. Only the outer serosa layer and the inner columnar luminal epithelial cells were affected by the reperfusion. However, a much faster and severe reperfusion damage of all uterine layers were evident during the reperfusion experiment following 48 hours of cold ischemia. This was indicated by major accumulation of extracellular fluid, presence of apoptotic-labeled glandular epithelial layer and vascular endothelium. A significant accumulation of lactate was measured in the perfusate with a subsequent decrease in pH. Conclusion: This model proved to be able to distinguish reperfusion injury-related differences associated to organ preservation. The experimental setup is a platform that can be used to conduct further studies on uterine ischemia- and reperfusion injury that may lead to improved human uterus transplantation protocols.
The development of uterine bio-scaffold using sheep uterus
( Yoojin Na ),( Tom Tristan Tiemann ),( Arvind Manikantan Padma ),( Jae Young Pae ),( Mats Brannstrom ),( Mats Hellstrom ),( Min Jong Song ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-
Objective: The aim of the present study was to establish adequate protocols for scaffold generation and prepare for future in vivo sheep uterus bioengineering experiments. Uterus tissue engineering may dismantle limitations in current uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularized scaffolds to restore fertility in a partially impaired uterus and now mandate experiments on larger and more human-like animal models. Methods: Three decellularization protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularization solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support fetal sheep stem cells after recellularization. Results: In all protocols, sheep uteri could successfully be decellularized while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favorable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Conclusion: The present study shows three valuable decellularization protocols for future in vivo sheep uterus bioengineering experiments.
( Joo Hee Yoon ),( Min Jong Song ),( Sung Jong Lee ),( Soo Young Hur ),( Mee Ran Kim ),( Mats Hellstrom ),( Mats Brannstrom ),( Jang Heub Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
목적: To develop a method for auto-transplantation in situ of the porcine uterus and to evaluate that sacrifice of deep uterine vessels might inhibit the viability of transplanted uterus. 방법: The bicornate uterus with the cervix and ovaries was dissected and isolated with its bilateral feeding and draining vessels. The uterine arteries were cannulated and the uterus was flushed with heparinized normal saline. It was stored at 4°C for 60 minutes during continuous flushing. The uterus was then placed in its original pelvic location and both superficial uterine arteries and veins were anastomosed end-to-end to their origin but both deep uterine arteries and veins was ligated. During approximately 60 minutes of reperfusion, the viability of uterus was evaluated. 결과: All of 2 auto-transplanted pigs were considered well flushed and were kept for more than 2 hours without re-anastomosis of deep uterine vessels. 결론: In this report on auto-transplantation in situ of the porcine uterus it is demonstrated the possibility that an acceptable reperfusion can be achieved without re-anastomosis of deep uterine vessels as like trachelectomy in woman with early cervical cancer. The small multiple uterine vessels need more time to perform re-anastomosis rather than kidney transplantation, which need just one artery, one vein and one ureteral re-anastomosis. So it is one of the hurldes against the human uterine transplantation. Furthermore, this trial suggested that our team could perform the small size vessels re-anastomosis. We should perform other animal trials as like sheep for the future success of human uterine transplantation in Korea.