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삼릉(三稜)이 자궁경부암세포(子宮頸部癌細胞)(HeLa cell)의 Apoptosis에 미치는 영향(影響)
홍기철 ( Ki Cheul Hong ),김주연 ( Joo Yeon Kim ),공복철 ( Bok Cheul Kong ),최창민 ( Chang Min Choi ),유심근 ( Sim Keun Yoo ) 대한한방부인과학회 2005 大韓韓方婦人科學會誌 Vol.18 No.4
Purpose: This study is to examine the ability of Rhizoma Scirpi (RS) to induce HeLa cell viability. Methods: We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below 37℃, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results: 1. RS induces mitochondria membrane potential collapse. 2. P38 MAPK is involved in RS-induced death in HeLa cells. 3. P38 MAPK is involved in RS-induced apoptosis in HeLa cells. 4. P38 MAPK reguates RS-induced caspase-3, -8 and -9 activation in HeLa cells. 5. The inhibition of caspase regulates RS-induced cell death in HeLa cells. 6. RS induces mitochondria membrane potential collapse in HeLa cells. 7. P38 MAPK is involved in the regulation of Bcl-2 and Bax in HeLa cells. 8. RS regulates the expression of Bcl-2 and Bax in HeLa cells. 9. SR induces p38 MAPK activation in HeLa cells. Conclusion: RS induces apoptosis in HeLa cells via p38 MAPK activation.
삼비탕(三痺湯)이 XO/HX에 의해 손상(損傷)된 골아세포(骨芽細胞)에 미치는 효과(效果)
박경환 ( Kyung-hwan Park ),이영석 ( Young-suck Lee ),강솔 ( Sol Kang ),조한백 ( Han-baek Cho ),김종환 ( Jong-hwan Kim ),유심근 ( Sim-keun Yoo ),홍기철 ( Ki-cheul Hong ) 대한한방부인과학회 2001 大韓韓方婦人科學會誌 Vol.14 No.3
To evaluate the effect of Sambitang(SBT) water extract on cultured osteoblast inhibited by xanthine oxidase(XO) and hypoxanthine(HX)- induced oxygen radicals, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay, Neutral red(NR) assay, thiobarbituric acid reactive substances(TBARS) assay and Lactate Dehydrogenase(LDH) activity assay were carried out after the cultured osteoblast were preincubated with various concentrations of SBT water extract for 3 hours prior to exposure of XO/HX. The results obtained were as follows: 1. XO/HX decreased the survival rate of the cultured osteoblast on NR assay and MTT assay. 2. SBT water extract have efficacy of decreasing lipid peroxidation. 3. SBT water extract have efficacy of increasing LDH activity. From above the results, It is concluded that SBT has marked efficacy as a treatment for the damages caused in the XO/HX-mediated oxidative process.