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Plant Regeneration from Callus and Adventitious Root Segments of Pulsatilla Koreana Nakai
최용의,Jung Su Jin1,Jeong Jae Hun,윤의수 한국식물생명공학회 2007 JOURNAL OF PLANT BIOTECHNOLOGY Vol.34 No.2
Plant regeneration of Pulsatilla koreana was achieved via adventitious shoot formation indirectly from callus and directly from adventitious root segments. For the callus induction from leaf or petiole explants, combination of 2,4- dichlorophenoxyaceticacid (2,4-D) with 2.22 µM 6-benzyladenine (BA) was effective. Adventitious shoot induction from callus was enhanced by the combined treatment with 0.1 µM polyvinylpyrrolidone (PVP) compared to cytokinin treatment alone. Adventitious roots were induced from the petiole segments on 1/2 MS medium with 4.93 µM IBA. High frequency direct adventitious shoot formation from the segments of adventitious roots was achieved on medium with 4.92 µM 2-isopentenyladenine (2-ip). Elongated shoots were rooted on half-strength MS medium containing 5.71 µM indole acetic acid (IAA). Regenerated plantlets with well-developed shoots and roots were successfully transferred to soil. This in vitro propagation protocol might be useful for mass propagation as well as conservation of this plant
한국 인삼 (Panax ginseng C.A. Meyer)의 자엽절편 배양으로부터 형성되는 체세포배의 기원
최용의,소웅영 한국식물생명공학회 1994 식물생명공학회지 Vol.21 No.3
Cotyledon segments of korean ginseng produced somatic embryos when cultured on MS basal medium, whereas plumule or excised axis explants did not. histological examination revealed that the cells in proximal region of cotyledon turned meristematic and densely cytoplasmic was composed of smaller and more densely cytiplasmic cells than the subepidermal cells. however, in the case both epidermis and subepidermal cells were almost the same in size and cytoplasmic density, the embryo originated from multiple cells. 성숙한 인삼 접합자배를 MS 기본배지에 배양하였을 때 대부분 발아되었다. 그런데 자엽을 절단하여 배양하였더니 높은 빈도로 체세포배가 발생되었다. 그러나 유경이나 하배축을 포함한 유근과 같은 부위에서는 체세포배가 발생되지 않았다. 조직학적 관찰에서 자엽의 기부 절단면 주변이 세포질이 농후해지고 분열하는 세포로 변화된 후 이들로부터 체세포배가 발생되었다. 체세포배의 기원은 표피가 하표피에 비해서 작고 세포질이 농후한 경우는 표피의 단세포에서 이루어졌고, 만일 표피 및 하표피세포가 그 크기나 세포질이 비교적 비슷한 경우는 표피와 하표피의 다세포에서 이루어졌다
최용의,이명종,유경하,배기화,한정연,이재선 한국산림과학회 2009 한국산림과학회지 Vol.98 No.2
There are remarkable differences in growth and morphological characters of roots between mountain and field cultivated Panax ginseng. Growth of root in mountain cultivated ginseng was much slower than that of field cultivated ginseng. However, the factor affecting the retarded growth in mountain ginseng was not known. Soil analysis revealed that phosphorus (P) content of mountain soil was exceptionally low at least ten-fold lower compared to that of field soil. Thus, we suggest that low availability of P in mountain soil may be one of the limiting factors for growth of ginseng in mountain soil environment. We had monitored the growth of ginseng plants after one and three years of phosphate fertilizer application. Three kinds of phosphate fertilizers: fused magnesium phosphate, fused superphosphate, and single superphosphate were applied to mountain soil. Application of phosphate fertilizers increased the fresh-, dry weight, and diameter of ginseng roots and resulted in increased P accumulation in roots. These results demonstrate that slow growth of ginseng in mountain soil environment might be attributed to the low P content in mountain soil. Thus, analysis of P amount in mountain soil will be a good indicator for the selection of suitable site the ginseng cultivation in forest.
가시오갈피 접합자배로부터 체세포배 발생 과정의 구조적 특성
최용의,소웅영 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-
가시오갈피 접합자배를 1.0 mg/L 2,4-D가 첨가된 MS 배지에 배양하였을 때, 배양재료 표면에서 직접 체세포배가 발생되었다. 접합자배를 온전한 상태로 배양하였을 때에 비하여 상처를 주어 배양하였을 때 배발생률이 증가되었으며, 부위별로 절단하여 배양하였을 경우 하배축에서 발생률이 높았다. 체세포배발생에 대한 조직학적 관찰 결과, 유근부위가 잘록한 단일배와 여러개의 배가 서로 융합된 다배가 관찰되었다. 이들 두가지 모양의 체세포배는 접합자배의 부위마다 발생빈도가 달랐는데, 자엽이나 하백축하부에서는 단일배가 주로 관찰되었고, 배형성능이 높은 부위인 자엽절이나 하배축상부에서는 다배의 발생률이 높았다. 단일배는 표피의 단세포 기원되었으나 다배는 모두 표피 및 하표피의 다수세포에서 기원되었음이 조직학적 관찰로 확인되었다. When the zygotic embryos of Acanthopanax senticosus were cultured on MS medium containing 1.0 mg/L 2,4-D, somatic embryos were produced directly from the surface without forming an intervening callus. The frequency of embryo formation was higher in the wounded zygotic embryos than in the intact ones. Hypocotyl explants showed a higher frequency of somatic embryo formation that cotyledon or radicle explants. Two typeds of somatic embryos were observed : one was single embryos with closed radicle which were mainly formed on cotyledon or radicle part; and the other was polyembryos of which hypocotyls were fused one another showing multicotyledons with a single hypocotyl. Histological observation revealed that single embryos originated from the single epidermal cells while polyembryos were derived from massive cells of epidermis of subepidermis.