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흰쥐 관절염 모델에서 용천 저출력 레이저 자극이 보행행동에 미치는 영향
지병욱 ( Byeong Uk Ji ),이성금 ( Cheng Jin Li ),이지은 ( Ji Eun Lee ),구성태 ( Sung Tae Koo ) 경락경혈학회 2015 Korean Journal of Acupuncture Vol.32 No.1
Objectives : The aim of the study is to investigate the effects of low level laser treatment (LLLT) on the gait behavior in the rat model of arthritis. Methods : Knee arthritis was induced by the injection of 125 μl of Complete Freund`s Adjuvant(CFA) into the right hind knee joint cavity. Arthritic rats were divided 3 groups; arthritis group was used for control(CON), 10 min of laser treated group (LSR10), and 30 min of laser treated group(LSR30). LLLT was applied to KI 1 for 11 times under gaseous anesthesia. We performed several analyses under catwalk test including stand and swing time, duty cycle of paw steps, intensity and print area of steps, and stride length. Results : Stand and duty cycle of paw steps were increased significantly at 12 days after arthritis induction in LSR30 group. Swing time was decreased significantly at 12 days after arthritis induction in LSR10 group. In the analysis of intensity, print area and stride length, however, results did not show statistical significance during the time point of experiments. Conclusions : The data suggest that LLLT on the rat model of CFA induced arthritis showed beneficial effects by increase of stand time and duty cycle of paw steps and decrease of swing time. Therefore, LLLT could be useful option to improve gait discomfort in arthritis patients.
붉은 덕다리버섯 발효 대조(大棗)의 예쁜꼬마선충 수명연장효과
지병욱 ( Byeong Uk Ji ),박성민 ( Sung Min Park ),구성태 ( Sung Tae Koo ),임병묵 ( Byung Mook Lim ),유영법 ( Young Beob Yu ) 경락경혈학회 2014 Korean Journal of Acupuncture Vol.31 No.4
Objectives : Zizyphus jujuba fruits(ZJF), a traditional Korean medicine has various biological activities such as anti-inflammatory, anti-oxidative and neuro-protective effects. However, it is still unclear whether ZJF has any biological effect on anti-aging. In this study, we examined the effect of ZJF on lifespan and thermal stress in C. elegans. Methods and Results : ZJF water extracts were fermented for 7 days(F7-ZJF) and 14 days(F14-ZJF) by Laetiporus sulphureus to increase secondary metabolites such as aglycone of flavonoids and terpenoids. In the lifespan assay, ZJF water extracts and fermented ZJF were treated on the agar medium plate with age synchronized egg stage of C. elegans. Treatment of F7-ZJF-200 μg/mL with OP-50 E. coli and F14-ZJF-200 μg/mL with OP-50 E. coli significantly increased life span of C. elegans(N2) at thermal stress condition of 25℃. Moreover mRNA levels of lifespan associated HSP 16.1, HSP 70, and HSF-1 were increased at thermal stress condition of 25℃. However, in the equilibration temperature of 20℃ after stress condition of 35℃ for 2 hr, F-14-ZJF-200 μg/mL treatment decreased the levels of heat shock protein in hsp16.2/GFP C. elegans. Conclusions : Our study indicates that prolong role of fermented-ZJF in C. elegans is mediated by control HSPs production.
추위에 노출된 슬관절염 모델에서 내슬안, 외슬안 직접구가 통증행동과 TRPM8 발현에 미치는 영향
지병욱 ( Byeong Uk Ji ),김이꽃 ( Yiquot Kim ),이지은 ( Ji Eun Lee ),구성태 ( Sungtae Koo ) 대한경락경혈학회 2016 Korean Journal of Acupuncture Vol.33 No.4
Objectives : The aim of the study is to investigate the effects of moxibustion on the pain behavior and expression of TRPM8 in the dorsal root ganglion(DRG) in the rat model of ambient cold(AC) exposed osteoarthritis(OA). Methods : OA was induced by the injection of 50 μl of 2% monosodium iodoacetate(MIA) into the knee joint cavity. To examine the level of pain, weight bearing forces(WBFs) of affected limb was measured. For the AC exposure, the animals were housed in 6 h/day at 4℃ for 14 days after MIA injection. Moxibustion treatment was performed at EX-LE4 and EX-LE5 with 5 cons(1, 7 or 10 mg) per day for 13 days from 5 days after MIA injection. The expressions of TRPM8 in DRG were measured by western blotting analysis. Results : The WBFs of MIA-AC group were decreased significantly compared to MIA group at 2, 3, 6, 7, 8 and 9 days after arthritis induction. After the first 6 h-AC exposure, expressions of TRPM8 in MIA-AC group were increased significantly compared to those of naive group. After moxibustion treatment, only the WBFs of 7 mg treated group were restored significantly. Moreover, the over-expressions of TRPM8 were attenuated by the moxibustion treatment in AC exposed rats. Conclusions : The data suggest that AC can increase arthritic knee pain via up-regulated TRPM8 and moxibustion treatment improve the arthritic pain via modulation of TRPM8 expression in DRG in the rat model of AC exposed MIA induced arthritis.