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Thiobacillus neapolitanus R-10 으로 부터 산화 효소의 분리 정제와 특성
원용돈,김동석,류병호 한국환경과학회 1996 한국환경과학회지 Vol.5 No.1
Thiobacillus neopolitanus R-10, which produces a active thiosulfate oxidase, was isolated from nightsoil. The optimal culture conditions of Thiobacillus neopolitanus R-10 for the production of enzyme was determined as followed: 0.8% Na_2S_20_3, 0.2% KH_2P0_4, 0.2% K_2HP0_4, 0.04% Na_2C0_3, 0.02% MgSO_4·7H_2O, 2ml trace elements solution, and pH 6.5 at 30℃ and 72hr cultivation. The oxidase was successively purified 83 folds yield by (NH_2)_2S0_4 fractionation, DEAE-Cellulose, Sephadex A-50 column chromatogrophy and gel Sephadex G-150 gel filteration with yield of 5.9%. The molecular weight of purified enzyme was estimated to be 43.000 dalton by SDS-polyacrylamide gel electrophoresis and gel filteration column chromatography. The enzyme activity was highest at 40℃ and pH 7.0 The enzyme activity was relatively high by β-mercaptoethanol but strongly inhibited by cysteine.
고정화 Thiobacillus neapolitanus R-10를 이용한 유황계 악취물질의 연속제거
원용돈,박상보 한국환경과학회 1995 한국환경과학회지 Vol.4 No.3
Continuous deodorization of malodorous sulfur compounds by Thiobaillus neapolitanusts R-10 immobilized onto a polypropylene pellet was studied using a column reactor at 30$^{\circ}$C. The maximum amounts of immobilized cells was 5.3 gall polypropylene with 5$\times$7.5mm in pellet size, and the amounts of immobilized cells in the higher part of the column was as twice as in the lower part. The optimum pH and temperature for removal of dimethyl sulfide were 6.0 and 30$^{\circ}$C, respectively. When 5-20 ${mu}ell$/l of hydrogen sulfide and methylmercaptan were employed 98% of removal efficiency were achieved. In contrast, lower concentrations of dimethyl sulfide and dimethyldisulfide should be supplied to meet satisfactory deodorization efficiency. The immobilized cell column was successfully operated for the deodorization of mixture of sulfur compounds over 15 days without significant loss of initial activity achieving high efficiency.
Thiobacillus neapolitanus R-10 에 의한 유황계 악취물질의 제거
원용돈,박상보,이원구,송승구 한국환경과학회 1995 한국환경과학회지 Vol.4 No.2
Thiobacillus neapolitanus R-10 isolated from sludge of night soil, showed an oxidizing activity on several malodorous sulfur compounds. The microbe successfully utilized hydrogen sulfide(H_2S), methy mercaptan(MM), dimethyl sulfide(DMS) and dimethyldisulfide(DMDS) during the batch culture reaction, of which H_2S was rather rapidly oxidized. To examine the ability for removal of malodorous sulfur compounds, various concentrations of sulfide substrates were supplemented separately to basal medium and their responses were investigated. As the concentration of sulfide was increased, growth was accelerated within three days of cultivation. 2.5mM was the most favorable substrate concentration of sulfide added for all cases tested. However, when the concentration of sulfur compounds were raised over 4mM, they behaved as a growth inhibitor.