미토콘드리아의 팽윤과 수축에 따른 분광흡광도와 미세구조간의 비연관성

부문종,이강오,김재진,최임순,Boo, Moon-Jong,Lee, Kang-Oh,Kim, Jae-Jin,Choe, Rim-Soon 한국현미경학회 1990 Applied microscopy Vol.20 No.2

Ultrastructural and spectrophotometric studies on mitochondrial swelling and contraction were carried out. All mitochondria just after isolated from rat liver showed condensed conformation. When mitochondria were incubated in 0.25 M sucrose only, they were moderately swollen and the absorbance at 520 nm was slightly decreased. Adding ATP to sucrose caused the absorbance to increase and the mitochondria to contract partially. KCl solution of 0.3 M induced marked decrease of absorbance and swelling of mitochondria. When ATP was added to KCl, increase of the absorbance and no contraction of the mitochondria excluding increased electron density of mitochondrial intermembranes were observed. Markedly decreased absorbance and somewhat largely swelled mitochondria in sodium arsenite solution of 0.4 or 1.0 mM were observed. When ATP was added to sodium arsenite, the absorbance increased slightly but mitochondria were more contracted than those in KCl-treated group. Above results indicate that the absorbance may not be correlated to morphological observations in the mitochondrial swelling and contraction.

간세포 미토콘드리아의 호흡에 미치는 비소의 영향

부문종,이강오,최임순,최치용 ( Moon Jong Boo,Kang Oh Lee,Rim Soon Choe,Chee Yong Choi ) 한국환경생물학회 1991 환경생물 : 환경생물학회지 Vol.9 No.2

To understand the effect of Arsenic(As) on the rat liver mitochondria, various amounts of As were added to the mitochondrial preparation. When pyruvate and malate were used as respiration substrate, the mitochondrial respiration was inhibited in the presence of 0.1∼1.0mM of As, but when succinate was used as substrate, the respiration was only inhibited at 1.0∼2.0mM of As. This suggests that As might inhibit NADH ubiquinone reductase(complex Ⅰ). 0.4 mM of As reduced the activity of complex Ⅰ and brought about induction of swelling and inhibition of ATP driven contraction of mitochondria. These experiments again suggest that As has an inhibitory effect on the mitochondrial respiration by selectively inhibiting the electron transport mediated by complex Ⅰ of respiratory chain and by affecting the integrity of mitochondrial membrane.

생쥐 간세포 Mitochondria의 전자전달계에 미치는 Chromium(VI)의 영향

부문종,유창규,최임순,Boo, Moon-Jong,Yoo, Chang-Kyu,Choe, Rim-Soon 한국현미경학회 1987 Applied microscopy Vol.17 No.1

To study hexavalent chromium effects on mitochondrial electron transport, the activities of electron transport enzymes and conformational change of mitochondria treated with $40{\mu}M$ of sodium dichromate ($Na_{2}Cr_{2}O_{7}\;2H_{2}O$) were investigated. And so were those of liver mitochondria isolated from mouse intraperitoneally injected with sodium dichromate, 40mg per kg body weight. On both treatment with chromium(VI), the activities of electron transfer enzymes (Complex I and IV) were increased to some extent and the ultrastructural transformation of mitochondria from a condensed to an orthodox conformation was inhibited under State IV respiration. These results represent' inhibitory effect of hexavalent chromium on electron transport without inhibiting electron transfer enzymes (Complex I and IV) in mitochondria. On intraperitoneal treatment with hexavalent chromium as sodium dichromate and trivalent chromium as chromic chloride, containing 37.5 mg of chromium per kg body weight, respectively, the activities of electron transfer enzymes of liver isolated from mouse with chromium(VI) was reduced, but that with chromium(III) was not affected. And with chromium(VI), all mice after 12 hours of treatment died, only after 6 hours survived. With chromium(III), however, all survived. This indicates that hexavalent chromium is more toxic than trivalent chromiumin mouse liver.

카드뮴과 비소의 생쥐 치사독성에 대한 카드뮴과 비소의 교차전처리효과

부문종(Moon Jong Boo) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.2

N/A Whether the pretreatment of sublethal arsenic or cadmium may prevent from lethality of arsenic or cadmium to mice, respectively, and also the protection against to lethality of arsenic or cadmium which might be induced by pregreatment of arsenic or cadmium may be related with their hepatic glutathione contents were investigated. When sodium arsenite or cadmium chloride was subcutaneously injected to mice(ICR strain) using lethal doses, all mice of both group were killed. The mortality of mice which were subsequently injected with lethal arsenic 24 hours after pretreatment of sublethal arsenic was decreased, and the same result was obtained in the case of cadmium. Sublethal pretreatment of arsenic or cadmium prior to lethal arsenic or cadmium treatment to mice, respectively, didn`t decrease hepatic glutathione contents of the survived mice, while decreases of that contents in liver were observed in the mice just after they died. Cadmium pretreatment decreased mortality of mice which subsequently injected with lethal arsenic, while arsenic pretreatment didn`t protect against cadmium lethality. These results indicate that protection against arsenic or cadmium lethality to mice induced by pretreatment of sublethal arsenic or cadmium may be directly related to other factors induced by sublethal camium pretreatment, not to hepatic glutathione contents.

카드뮴 전처리에 의한 생쥐의 카드뮴 치사 완화효과와 간 glutathione 함량과의 상관성

부문종(Moon Jong Boo) 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.1

Which sublethal cadmium pretreatment may prevent from lethal cadmium`s killing mice and which cadmium pretreatment-induced protection against cadmium lethality to mice may be related with their hepatic glutathione contents were investigated. When cadmium chloride was subcutaneously injected to mice(ICR strain) at various doses, all mice died, which treated with cadmium at dose of 300 μmoles/㎏ or more, and none died, which treated with cadmium at dose of 80 μmoles/㎏ or less. Subcutaneous pretreatment of sublethal cadmium decreased sacrifice of mice which subsequently injected with lethal cadmium, with most effectiveness at pretreatment dose of cadmium of 40 μmoles/㎏ b.w. and at 48 hours of interval between sublethal cadmium pretreatment and lethal cadmium treatment. Even if a great part of the cadmium-pretreated mice were sacrificed while treated with lethal cadmium, they survived longer than the non-pretreated mice. Sublethal cadmium pretreatment(40 μmoles/㎏ b.w.) 48 hours before lethal cadmium treatment to mice didn`t decrease hepatic glutathione contents of the survived mice, while decreases in the glutathione in livers were observed in the mice just after died. These results indicate that sublethal cadmium pretreatment-induced protection against cadmium lethality to mice may be related to their hepatic glutathione contents. [Cd pretreatment, Cd lethality, Hepatic glutathione contents].

비소의 급성처리가 흰쥐 간세포의 미세구조와 단백질 합성양상에 미치는 영향

최임순,부문종,노미경,조자영 ( Rim Soon Choe,Moon Jong Boo,Mi Kyeong Ro,Ja Yeoung Cho ) 한국환경생물학회 1991 환경생물 : 환경생물학회지 Vol.9 No.1

The acute effects of arsenic intoxication on hepatocytes were investigated. When cytosol fraction of arsenic-treated rat liver was eluted through Sephacryl S-200 gel filtration chromatography, six specific peaks were observed and six stress proteins were detected on the SDS-PAGE. GSH level decreased at 3 hrs after arsenic injection but increased at 6 hrs. However, it decreased again at 12 hrs. The hepatocytic ultrastructures of rats treated with arsenic changed gradually in the nuclear chromatin and mitochondria. This result suggests defects in cellular organelles by arsenic treatment.

한국환경생물학회 1990년도 추계학술대회 : 일반 연제 초록 ; 흰쥐 간장과 신장세포에 미치는 비소의 아급성 중독 영향

김충현,부문종,최임순 한국환경생물학회 1990 환경생물 : 환경생물학회지 Vol.8 No.2

$Cr^{6+}$가 흰쥐 신장세포내 미토콘드리아의 호흡에 미치는 영향과 저분자 크롬결합물질의 작용

강수길,부문종,최임순,Kang, Soo-Gil,Boo, Moon-Jong,Choe, Rim-Soon 한국현미경학회 1989 Applied microscopy Vol.19 No.2

To investigate the effects of hexavalent chromium on mitochondrial respiration of rat kidney, various hexavalent chromium concentrations were treated, then respiration and electron transfer enzyme activities were measured. Ultrastructural changes at state IV respiration of mitochondria were also observed. Then, to investigate protective role against hexavalent chromium in the body, low-molecular-weight, chromium-binding substances (LMCr) were purified from livers of rabbits 2hr after intravenously administrated with sodium dichromate at a dose of 74mg per kg body weight. And then, respiration rates of mitochondria treated with LMCr, hexavalent chromium containing 0.7mM chromium were measured. Hexavalent chromium decreased state IV respiration rates and electron transfer enzyme activities of mitochondria, and increased labile membrane and swelling. And partial inhibitions of condensed to orthodox conformational change were observed. Respiration rates of mitochondria treated with LMCr containing 0.7mM chromium did not differ from that of the non-treated mitochondria. But respiration rates of 0.7mM hexavalent chromium-treated mitochondria decreased by 42%, compared to non-treated mitochondria. These results suggest that LMCr may play an important role in detoxification of toxic hexavalent chromium.

흰쥐 신장세포에 미치는 비소의 아급성 중독 영향

김충현,부문종,최임순 ( Chung Hyon Kim,Moon Jong Boo,Rim Soon Choe ) 한국환경생물학회 1990 환경생물 : 환경생물학회지 Vol.8 No.1

The subacute effects of arsenic intoxication on renal cells were investigated. Aqueous sodium arsenite (NaAsO_2) solution containing 4ppm of trivalent arsenic was administered to male rats (Sprague-Dawley) for 15 days as drinking water. The ultrastructure and mitochondrial respiration of As-treated renal cell were not much different from that of the control. However, seven stress proteins were newly detected on the SDS-PAGE in the cytosol fraction of As-treated rat kidney. When the cytosol fraction of As-treated rat kidney was eluted through Sephadex G-75 gel filtration chromatography, one specific peak of AsBS was observed. Control group showed same peak. When AsBS was eluted at 0.10∼0.15 M NaCl concentration of Sephadex A-25 ion exchange chromatography, the absorbance of 250㎚ was more than that of 280㎚ in these fractions.

흰쥐 간조직의 세포내 비소결합물질의 특성에 관한 연구

최임순,부문종,김충현 한국통합생물학회 1990 동물학회지 Vol.33 No.4

흰쥐 간세포에 존재하는 비소결합물질의 특성을 조사하기 위하여 4 ppm의 비소를 포함하는 NaASO$_2$수용액의 식수로 15일간 흰쥐에 공급하였다. 비소처리군의 간 cytosol분획의 정상단백 질의 함량은 감소하였으나 8종류의 stress protein의 함량은 증가하였다. Cytosol에 존재하는 비소결합체물질은 한 종류이고,glycine, glutamic acid 및 cysteine의 3종류의 아미노산으로 구성되어있으며 분자량은 500D이었다.Glutathione은 비소와 5:1의 몰비로 결합하였으며 glutathione과 비소의 복합체는 gel filtration chromatography에서 비소결합물질과 같은 이동성을 나타내어 cytosol에 존재하는 미소결합물질은 glutathione으로 추정되었다. Glutathione에 결합한 비소는 미토콘드리아의 호흡, 형태전환 및 팽윤과 수축기능에 있어서 대조군과 별다른 차이를 나타내지 않았다. 이와 같은 결과들로써, 생체에 직접 처리한 비소에 의하여 stress protein합성이 촉진되며, 간 cytosol에 존재하는 비소결합물질은 glutathione으로 추정되고, 비소와 복합체를 형성함으로써 비소의 세포독성에 대한 방어기능을 나타내는 것으로 생각된다. Sodium arsenite (As) aqueous solution containing 4 ppm wss given to male rats for 15 days as drinking water. Electrophoretic pattern of liver cytosol from As-fed rats appeared to be significantly different from that of the control rats. Although the normal protein content of the cytosol fraction of As-fed rat liver was decreased, 8 stress proteins were increased. In liver cytosol fractions of As-treated rat, one kind of arsenic-binding substance (AsBS) was observed. Molecular weight of AsBS was identified to be 500 D and composition of amino acid was glycine, glutamic acid and cysteine. Glutathione (GSH) appeared to bind to arsenic and GSH-As complex showed the same mobility as AsBS on gel filtration chromatography. GSH conjugated As prevented As from inhibiting respiration, conformational change and swelling-contration of mitochondria. According to the above results. it is concluded that in vfuo treated arsenic stimulated synthesis of stress protein, and arsenic-binding substance might be glutathione and have a protective role against arsenic toxicity.