http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Sang Gon,Kim, Sun Tae,Wang, Yiming,Kim, Sung-Kun,Lee, Chang Hoon,Kim, Keun-Ki,Kim, Ju-Kon,Lee, Sang Yeol,Kang, Kyu Young Blackwell Publishing Ltd 2010 Physiologia plantarum Vol.138 No.1
<P>Isoflavone reductase is an enzyme involved in isoflavonoid biosynthesis in plants. However, rice isoflavone reductase-like gene (<I>OsIRL,</I> accession no. AY071920) has not been unraveled so far. Here, we have characterized its behavior in response to oxidizing agents. Using Northern and Western blot analyses, the <I>OsIRL</I> gene and protein were shown to be down-regulated in young seedling roots treated with reduced glutathione (GSH) and diphenyleneiodonium (DPI), known quenchers of reactive oxygen species (ROS). The <I>OsIRL</I> transcript level in rice suspension-cultured cells was also found to be induced by oxidants such as hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>), ferric chloride (FeCl<SUB>3</SUB>), methyl viologen (MV) and glucose/glucose oxidase (G/GO), but down-regulated when co-treated with GSH. Furthermore, to investigate whether overexpression of <I>OsIRL</I> in transgenic rice plants promotes resistance to ROS, we generated transgenic rice lines overexpressing the <I>OsIRL</I> gene under an abscisic acid (ABA) inducible promoter. Results showed that the <I>OsIRL</I> transgenic rice line activated by ABA treatment was tolerant against MV and G/GO-induced stress in rice leave and suspension-cultured cells. Our results strongly suggest the involvement of <I>OsIRL</I> in homeostasis of ROS.</P>
Kim, Won-Kon,Bae, Kwang-Hee,Choi, Hye-Ryung,Kim, Do-Hyung,Choi, Kwang-Soo,Cho, Yee-Sook,Kim, Hee-Dai,Park, Sung-Goo,Park, Byoung-Chul,Ko, Yong,Lee, Sang-Chul Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.4
Protein tyrosine phosphatases (PTPs) are pivotal regulators of key cellular functions, including cell growth, differentiation, and adhesion. Previously, we reported that leukocyte common antigen-related (LAR) tyrosine phosphatase promotes osteoblast differentiation in MC3T3-E1 preosteoblast cells. In the present study, the mechanism of the regulatory action of LAR on osteoblast differentiation was investigated. The mineralization of extracellular matrix and calcium accumulation in MC3T3-E1 cells were markedly enhanced by LAR overexpression, and these effects were further increased by treatment with an MEK inhibitor. In addition, LAR overexpression dramatically reduced extracellular signal-regulated kinase (Erk) activation during osteoblast differentiation. In contrast, a marginal effect of the inactive LAR mutant on Erk activation was detected. Expression of osteoblast-related genes such as ALP, BSP, DLX5, OCN, and RUNX2, was increased by LAR overexpression during osteoblast differentiation. On the basis of these results, we propose that LAR functions as a positive regulator of osteoblast differentiation by modulating ERK activation. Therefore, LAR phosphatase could be used as a novel regulatory target protein in many bone-associated diseases, including osteoporosis.
Thickness Dependence of Properties of ITO Films Deposited on PET Substrates
Kim, Seon Tae,Kim, Tae Gyu,Cho, Hyun,Yoon, Su Jong,Kim, Hye Sung,Kim, Jin Kon American Scientific Publishers 2016 Journal of nanoscience and nanotechnology Vol.16 No.2
<P>Indium tin oxide (ITO) films with various thicknesses from 104 nm to 513 nm were prepared onto polyethylene terephthalate (PET) substrates by using r.f. magnetron sputtering without intentionally heating the substrates. The structural, optical, and electrical properties of ITO films were investigated as a function of film thickness. It was found that the amorphous nature of the ITO film was dominant below the thickness of about 200 nm but the degree of the crystallinity increased with an increasing thickness above the thickness of about 250 nm, resulting in the increase of carrier concentration and therefore reducing the electrical resistivity from 5.1x10(-3) to 9.4x10(-4) Omega . cm. The average transmittance (400-800 nm) of the ITO deposited PET substrates decreased as the film thickness was increasing and was above 80% for the thickness below 315 nm. The results show that the improvement of the film crystallinity with the film thickness contributes to the increase of the carrier concentration and the enhancement of the electrical conductivity.</P>
Patient dose measurements in diagnostic radiology procedures in Korea
Kim, You-hyun,Choi, Jong-hak,Kim, Chang-kyun,Kim, Jung-min,Kim, Sung-soo,Oh, Yu-whan,Lee, Chang-yeap,Kang, Dae-hyun,Lee, Young-bae,Cho, Pyong-kon,Kim, Hyung-chul,Kim, Chel-min Nuclear Technology Publishing 2007 Radiation Protection Dosimeetry Vol.123 No.4
Kim, Sung-Kon,Kim, Dong-Gyun,Lee, Aeri,Sohn, Hae-Sung,Wie, Jeong Jae,Nguyen, Ngoc A.,Mackay, Michael E.,Lee, Jong-Chan American Chemical Society 2012 Macromolecules Vol.45 No.23
<P>A series of organic/inorganic hybrid block and random copolymers were prepared by reversible addition–fragmentation chain transfer (RAFT) polymerization using poly(ethylene glycol) methyl ether methacrylate (PEGMA) and 3-(3,5,7,9,11,13,15-heptaisobutylpentacyclo[9.5.1.1<SUP>3,9</SUP>.1<SUP>5,15</SUP>.1<SUP>7,13</SUP>]octasiloxane-1-yl)propyl methacrylate (MA-POSS) as monomers in order to study the effect of polymer morphology and POSS content on the properties of polymer electrolytes. Flexible and dimensionally stable free-standing films were made from the hybrid block and random copolymers mixed with lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) when the contents of MA-POSS unit were larger than 31 and 16 mol %, respectively. The ionic conductivity of the solid-state block copolymer (PBP) electrolyte was found to be 1 order of magnitude higher than that of the random copolymer (PRP) electrolyte when they had similar MA-POSS content, although their glass transition temperature values of their ion-conducting segments were quite close. Moreover, the ionic conductivity of the PBP electrolyte was not much different from that of the wax state poly(poly(ethylene glycol) methyl ether methacrylate) (P(PEGMA)) electrolyte. For example, the ionic conductivity values for the PBP electrolyte containing 31 mol % of MA-POSS, the PRP electrolyte containing 29 mol % of MA-POSS, and P(PEGMA) electrolyte were 2.05 × 10<SUP>–5</SUP>, 3.00 × 10<SUP>–6</SUP>, and 4.23 × 10<SUP>–5</SUP> S cm<SUP>–1</SUP>, respectively, at 30 °C. The large ionic conductivity value of the block copolymer electrolyte is ascribed to the nanophase separation forming the ion-conducting channels.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mamobx/2012/mamobx.2012.45.issue-23/ma301404q/production/images/medium/ma-2012-01404q_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ma301404q'>ACS Electronic Supporting Info</A></P>
The Urate-lowering Efficacy and Safety of Febuxostat in Korean Patients with Gout
( Sung Hwan Park ),( Yeong Wook Song ),( Won Park ),( Eun Mi Koh ),( Bin Yoo ),( Soo Kon Lee ),( Dae Hyun Yoo ),( Yun Jong Lee ),( Hyun Ah Kim ),( Hyo Jin Choi ),( Ho Youn Kim ),( Hyong Gi Jung ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.4
Objective. To compare the urate-lowering efficacy and the safety of febuxostat, allopurinol and placebo in Korean patients with gout for 4 weeks. Methods. Subjects (n=182) with gout were randomized to febuxostat (40, 80, 120 mg), allopurinol 300 mg, or placebo group. The primary end point was the proportion of subjects whose serum urate concentration fell to less than 6.0 mg/dL after the 4-week treatment. Results. The primary end point was reached at 25.7%, 80.0% and 83.3% of patients receiving 40, 80 and 120 mg of febuxostat, respectively, 58.3% of those receiving 300 mg of allopurinol and none of the placebo (p<0.001: each febuxostat dose or allopurinol group versus placebo group, p=0.0484 and p=0.0196: febuxostat 80 and 120 mg compared with allopurinol, respectively). The number and proportion of subjects who developed adverse events (AEs) were 13 subjects (37%), 14 (39%) and 18 (50%) in the febuxostat of 40, 80 and 120 mg group, respectively, 21 (57%) in the allopurinol 300 mg group and 17 (46%) in the placebo group. No statistically significant differences in the incidence rates of adverse events were observed between the groups. There was no significant difference in gout flare-up incidence. Conclusion. Febuxostat, 80 mg or 120 mg, was more effective than allopurinol (300 mg) or placebo, when lowering the serum urate. The safety of febuxostat and allopurinol was comparable.
Kim, Won-Kon,Jung, Hyeyun,Kim, Do-Hyung,Kim, Eun-Young,Chung, Jin-Woong,Cho, Yee-Sook,Park, Sung-Goo,Park, Byoung-Chul,Ko, Yong,Bae, Kwang-Hee,Lee, Sang-Chul Cambridge University Press 2009 Journal of cell science Vol.122 No.22
<P>Mesenchymal stem cells (MSCs) are multipotent adult stem cells that can differentiate into a variety of mesodermal-lineage cells. MSCs have significant potential in tissue engineering and therapeutic applications; however, the low differentiation and proliferation efficiencies of these cells in the laboratory are fundamental obstacles to their therapeutic use, mainly owing to the lack of information on the detailed signal-transduction mechanisms of differentiation into distinct lineages. With the aid of protein-tyrosine-phosphatase profiling studies, we show that the expression of leukocyte common antigen related (LAR) tyrosine phosphatase is significantly decreased during the early adipogenic stages of MSCs. Knockdown of endogenous LAR induced a dramatic increase in adipogenic differentiation, whereas its overexpression led to decreased adipogenic differentiation in both 3T3-L1 preadipocytes and MSCs. LAR reduces tyrosine phosphorylation of the insulin receptor, in turn leading to decreased phosphorylation of the adaptor protein IRS-1 and its downstream molecule Akt (also known as PKB). We propose that LAR functions as a negative regulator of adipogenesis. Furthermore, our data support the possibility that LAR controls the balance between osteoblast and adipocyte differentiation. Overall, our findings contribute to the clarification of the mechanisms underlying LAR activity in the differentiation of MSCs and suggest that LAR is a candidate target protein for the control of stem-cell differentiation.</P>