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Neospora caninum에 자연 감염된 임신우로부터 유산된 태아의 병리조직학적 관찰
손정훈(Jeong-Hoon Son),조성환(Sung-Whan Cho) 한국생명과학회 2010 생명과학회지 Vol.20 No.10
충남 공주시와 연기군의 목장 4곳에서 Neospora에 자연 감염된 임신우로부터 유산된 태아를 병리조직학적, 전자현미경적으로 관찰하였다. 유산된 태아는 전신 부종과 함께 부검시 복강과 흉강은 혈액성ㆍ장액성 액체로 충만되어 있었다. 광학현미경적으로 심장과 간에서는 충ㆍ출혈 및 심근세포와 간세포의 괴사를 동반한 대식 세포, 임파구, 단핵구 세포로 구성된 많은 수의 염증세포 침윤이 관찰되었고, 간에서는 간세포의 세포질과 간질 조직에서 tachyzoite 집락이 관찰되었다. 뇌에서는 신경세포내와 주위에서 크기가 다양한 많은 수의 조직낭포가 관찰되었고, 조직낭포는 구형으로 두꺼운 막으로 싸여 있었으며 내부에는 많은 bradyzoite를 포함하고 있었다. 또한, 뇌조직에서는 충ㆍ출혈 및 국소성 괴사와 함께 광범위한 신경교증이 관찰되었고 국소성 괴사 부위 주위와 혈관주위에 소교세포로 구성된 많은 수의 염증세포 침윤이 관찰되었다. 전자현미경적으로 조직낭포의 막 두께는 대략 1 ㎛ 로 모양은 불규칙하였고, 내부에는 길이 2-5 ㎛, 폭 1-2 ㎛인 100여 개의 bradyzoite가 관찰되었다. Bradyzoite의 핵은 posterior tip으로부터 1-1.5 ㎛ 전방에 존재하였고, amylopectin 과립, 전자밀도가 높고 작은 구형인 과립과 전자밀도가 상대적으로 낮으나 큰 구형의 과립, 전자밀도가 균질한 rhoptry 및 zoite pellicle에 대해 수직방향으로 달리는 microneme이 핵과 posterior tip 사이의 세포질 내에서 관찰되었다. 이상의 본 연구는 ELISA검사를 통해 Neospora 항체에 양성반응을 나타내는 임신우로부터 유산된 태아에서 발견된 조직낭포를 전자현미경적으로 관찰하여 N. caninum임을 확인하였다. This study examined the histopathologic and electron microscopic findings of aborted fetuses from pregnant dairy cows naturally infected with Neospora caninum (N. caninum) at four farms in Gongju city and Yeonki gun of Choongnam province. Systemic subcutaneous edema was observed in the aborted fetuses. The necropsy revealed considerable serosanguinous fluid in the body cavity of the aborted fetuses. Light microscopy showed the infiltration of many inflammatory cells consisting of macrophages, lymphocytes and mononuclear cells, accompanied by congestion, hemorrhage and necrosis of myocardiac cells and hepatocytes in the liver and heart of the aborted fetuses. In the liver, clusters of tachyzoites were formed in the cytoplasm of hepatocytes and the interstitial tissue. In the brain, many tissue cysts of various sizes were observed in the nerve cells and their adjacent areas. Tissue cysts had a round shape and contained a large amount of bradyzoite. In addition, there was diffuse gliosis accompanied by congestion and hemorrhage and focal necrosis in the brain. Infiltration of microglial cells were observed at the periphery of the focal necrosis and perivascular area in the brain. Electron microscopy showed that the tissue cyst wall had a thickness of approximately 1 ㎛ with an irregular shape. On the interior side, more than 100 bradyzoites with lengths of 2-5 ㎛ and widths of 1-2 ㎛ were observed. The nucleus of in the bradyzoites was located approximately 1-1.5 ㎛ anterior to the posterior tip of the zoite. In the cytoplasm between the nucleus and the posterior tip, there were many amylopectin granules, electron-dense small-sized and electron-thin large-sized round granules, homogeneously electron-dense rhoptries and micronemes oriented perpendicularly to the zoite pellicle. To summarize, tissue cysts were identified on electron microscopy from the aborted fetus from N. caninum seropositive pregnant cow by the ELISA. This led to the confirmed presence of N. caninum.
EFFECTS OF TEMPERATURE ON VIABILITY OF SARCOCYSTS OF Sarcocystis cruzi IN CARDIAC MUSCLE OF CATTLE
노재운(Jae Woog NOH),장두환(Du Hwan JANG),강영배(Yung Bai KANG),장환(Hwan JANG),위성환(Sung Hwan WEE) 한국예방수의학회 1988 예방수의학회지 Vol.12 No.2
돼지의 Sarcocystis miescheriana와 소의 S. cruzi는 사람을 종숙주로 할 수 있으며 이들은 사람이나 동물이 감염력을 가진 cyst를 함유한 고기를 날것이나 덜 조리된 상태로 먹을 때 감염이 일어난다. 그러므로 Sarcocystis의 전파를 막는 한 방법으로써 식육내에 감염력이 있는 cyst를 파괴하는 것이다. 본 연구는 식육내에 있는 Sarcocystis의 감염력이나 생존력이 식육의 처리온도와 시간에 따라 어떻게 변화하는가를 조사하였으며 생존력은 0.5% trypsin 소화법에 의해 검출되는 bradyzoites의 유무로 판단하였다. 소의 심근을 -20℃와 -10℃에 90분 그리고 -5℃에 17시간 보존시 감염력이 상실되었으나 0~4℃에서는 11일간 보존시에도 감염력을 가진 것으로 나타났다. 40℃에서 17시간동안 처리된 심근내의 Sarcocystis는 파괴되었으며 60℃, 100℃에서는 5분과 2분의 짧은 처리시간에서 감염력이 상실되었다.
도축우(屠畜牛)의 심장근육내(心臟筋肉內) 주육포자충(住肉胞子蟲) 감염실태조사(感染實態調査)와 Sarcocystis cruzi의 동정(同定)
박양주 ( Yang Joo Park ),김종술 ( Jong Sool Kim ),정동수 ( Dong Su Chung ),박양순 ( Yang Soon Park ),신명균 ( Myung Kyun Sin ),김교승 ( Kyo Seung Kim ) 한국가축위생학회 1992 韓國家畜衛生學會誌 Vol.15 No.2
330 Samples of the slaughtered cattle heart muscle were collected from the abattoirs of five regions in Kangwon-do to reveal the frequency of sarcocystis infections during January through December in 1991. The samples were inspected for bradyzoites by the trypsin digestion technique and the possitive samples were fed to dogs and cats for the detection of sporocysts shed in the feces. The results obtained were summarized as follows: 1. The infection rate of bovine Sarcocystis investigated from 330 samples was 43.6%. 2. It revealed that the infection rate of sarcocystis increased gradully with the advance in the age, 14.5% in below two years, 26.1% in the three years, 30% in four years, 54.7% in five years, 74.4% in six years, 90% in seven years and 100% in older than eight years. 3. The cyst walls detected out from the heart muscles were less than 1μm in thickness and the size of bradyzoites were 11.8×2.8μm in average. 4. The size of sporocysts shed in the feces of dogs were 15.8×9.8μm in average and the prepatent periods ranged from 12 to 16days. 5. Sarcocystis found in the bovine heart muscles were identified as Sascocystis cruzi(Hasselman, 1923), wenyon, 1926.
Toxoplasmic meningoencephalitis in a stray cat in Korea
Kim, Ha-Young,Bae, You-Chan,Woo, Gye-Hyeong,Byun, Jae-Won,Jung, Byeong-Yeal,Park, Jung-Won,Chae, Hee-Sun,Choi, Jae-Yong,Nakayama, Hiroyuki,Hwang, Eui Kyung,Joo, Yi-Seok,Lee, O-Soo The Korean Society of Veterinary Science 2009 大韓獸醫學會誌 Vol.49 No.4
A dead stray cat was necropsied for zoonotic feline disease monitoring. Grossly, there were no specific lesions. Major microscopic lesions included lymphocytic meningoencephalitis, malacia, and tissue cysts in the cerebral and cerebellar cortex. The size and shape of tissue cysts were identical to those of Apicomplexa including Toxoplasma (T.) gondii. Bradyzoites in the tissue cyst were strongly positive for T. gondii by immunohistochemistry. Electron microscopy revealed that bradyzoites within the tissue cyst were similar to the morphological features of T. gondii. Fresh tissue samples were examined by a polymerase chain reaction assay and resulted in a specific band of T. gondii only in the brain. Based on the results, this case was diagnosed as toxoplasmosis. This is the first case of toxoplasmic meningoencephalitis in a cat in Korea.
국내(國內) 돼지의 Sarcocystis 감염과 Sarcocystis종의 동정에 관한 연구
문무홍,Moon, Moo-hong 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.3
Five hundred and forty-eight samples of pig heart muscle were collected from the abattoirs of many regions in Korea to reveal the frequency of Sarcocystis infections and to identify the species from June 1988 to April 1989. Heart muscle of the pigs was inspected for sarcocysts by the direct detection technique and for bradyzoites by the trypsin digestion technique. For examination of development of the parasites in the final host, 5 cross bred mature dogs, 5 puppies and 5 kittens were fed 100g, 50g and 50g of the infected meat respectively, four times in 2 days. Of 402 fattened and 146 older culled breeding pigs, 3 fattened pigs and 39 culled pigs were positive for Sarcocystis. Sarcocystis cysts from heart muscle measured an average of $425{\times}169{\mu}m$ and bradyzoites an average of $15.6{\times}3.5{\mu}m$. Of 15 animals, only 2 puppies were infected with Sarcocystis. The prepatent period was 11 to 12 days and patent period was not examined since the puppies were infected with some another infections and one died on day 11 and another died on day 12 after ingestion of the meat. The sporulated oocysts were detected 11 days after ingestion of the meat and sporocysts 12 days from the puppy feces. The sporulated oocysts measured an average of $16.5{\times}11.5{\mu}m$ and sporocysts an average of $12.6{\times}7.9{\mu}m$. On scraping examination of the intestinal mucosa, fully sporulated oocysts were detected in the tip of the intestinal villi. Considering above all descriptions, Sarcocystis in pig heart muscle in Korea was identified with Sarcocystis suicanis.
Jong Sool KIM(金淙述),Ki Soo CHUNG(鄭基洙),Dong Hoon KIM(金東勳),Tae Jong KIM(金太鍾),Yung Bai KANG(姜英培) 한국예방수의학회 1989 예방수의학회지 Vol.13 No.3
The Swine Sarcocystis were investigated the hearts of 524 slaughtered pigs in the area of Kangwon-do. The rate of Sarcocystis infetion and the morphologic states of the cyst bradyzoite were ivestigated. The results obtained were summarized as follow: 1. The infection rate of swine Sarcocystis investigated 384 breedings pigs were found to be 16.7%. 2. The swine Sarcocystis were observed only the pigs over third parturition(19.8%: 64 herds,∴p<0.01). 3. The cyst walls detected out from the cardiac muscle were less than 1㎛ in thickness and the size of bradyzoites were 15.7×3.7㎛ in average. 4. The bananashape and ellipical micropore on the surface in the bradyzoites were observed with the scanning electrone microscope.
도축우(屠畜牛)에 있어서 주육포자충(住肉胞子蟲) 감염실태조사(感染實態調査)
강영배,김상희,위성환,Kang, Yung-bai,Kim, Sang-hee,Wee, Sung-hwan 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2
A preliminary survey of Sarcocystis species in the slaughtered cattle in Seoul was undertaken during October through December 1987, using digestion diagnostic technique for the heart muscle specimens digested in 0.5% trypsin solution. Results indicated that 41.5% of 159 bovine hearts were infected with Sarcocystis proliferative forms. High frequencies in the exotic dairy cattle(42.2%) and the Korean native cattle(41.7%) were noticed in comparison with low frequency in the cross breed(25.0%). No differences were indicated between the sexes of the host animals, although an age difference was noticed as 48.9% in cattle older than four years in comparison with 39.0% and 37.1% in younger than two years and in two to four years, respectively.