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        김미경,노경아 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

        This study was performed to investigate effects of examination-stress and protein supplementation on nitrogen metabolism and blood protein levels of Korean college students. Experiment was conducted at the beginning of a academic term and during midterm exmination. During midterm examination, subjects were classified into two groups randomly: protein supplemental group(male n=6, female n=10)and placebo group(male n=4, female n=9). Protein capsules(2g/day)above 10% of indispensible amino acids requirement estimates were given to supplemental group for 10 days. At the beginning of the term, male students(n=12)ingested 223.15mgN/kg/d, excreted 20.74mgN/kg/d in feces, and excreted 94.31mgN/kg/d in urine. Their apparent protein digestibility was 90.72%, true N balance was +100.11mgN/kg/d, and the mean maintenance N requirement of mixed Korean diet calculated was 112.13mgN/kg/d. Female students(n=19)ingested 171.44mgN/kg/d, excreted 22.13mgN/kg/d in feces, and excreted 122.92mgN/kg/d in urine. Their apparent protein digestibility was 86.76%, true N balance was +18.39mgN/kg/d, and the mean maintenance N requirement calculated was 135.31mgN/kg/d. Blood levels of serum total protein, albumin, and BUN were within normal range. During midterm exmination, fecal and urinary N excretions of female subjects(n=19)were increased, especially urea N markedly, and urea N/creatinine N ratio was augumented significantly. Apparent protein digestibility of male subjects(n=10)was decreased. Examinationstress showed 8.05mgN/kg/d(7.2%)increase of mean maintenance N requirement in male and 8.55mgN/kg/d(6.3%)increase in female students in comparison with that of the beginning of the term. Serum total protein and albumin levels showed no significant change, but serum transferrin level of female were decreased significantly. During midterm examination, females supplemented with protein capsules(2g/d)had no significant increase in fecal and urinary N excretions.

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