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Cho, E.,Choi, S.,Shim, J.,Kim, T.,Shin, R.,Lee, J.,Kim, J.,Jung, H.I.,Kang, S. Elsevier Sequoia 2016 Sensors and actuators. B Chemical Vol.233 No.-
We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.