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- 주제어 : Histological Labeling (검색결과 1 건)
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윤웅배,김현진,김광기,최용두,장희진,손대경 대한의료정보학회 2016 Healthcare Informatics Research Vol.22 No.3
Objectives: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining. Methods: We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods. Results: An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging. Conclusions: The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
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