Coating gold nanoparticles to a glass substrate by spin-coat method as a surface-enhanced raman spectroscopy (SERS) plasmonic sensor to detect molecular vibrations of bisphenol-a (BPA)

Eskandari, Vahid,Hadi, Amin,Sahbafar, Hossein Techno-Press 2022 Advances in nano research Vol.13 No.5

Bisphenol A (BPA) is one of the chemicals used in monomer epoxy resins and polycarbonate plastics. The surface-enhanced Raman spectroscopy (SERS) method is precise for identifying biological materials and chemicals at considerably low concentrations. In the present article, the substrates coated with gold nanoparticles have been studied to identify BPA and control the diseases caused by this chemical. Gold nanoparticles were made by a simple chemical method and by applying gold salt and trisodium citrate dihydrate reductant and were coated on glass substrates by a spin-coat approach. Finally, using these SERS substrates as plasmonic sensors and Raman spectroscopy, the Raman signal enhancement of molecular vibrations of BPA was investigated. Then, the molecular vibrations of BPA in some consumer goods were identified by applying SERS substrates as plasmonic sensors and Raman spectroscopy. The fabricated gold nanoparticles are spherical and quasi-spherical nanoparticles that confirm the formation of gold nanoparticles by observing the plasmon resonance peak at 517 nm. Active SERS substrates have been coated with nanoparticles, which improve the Raman signal. The enhancement of the Raman signal is due to the resonance of the surface plasmons of the nanoparticles. Active SERS substrates, gold nanoparticles deposited on a glass substrate, were fabricated for the detection of BPA; a detection limit of 10-9 M and a relative standard deviation (RSD) equal to 4.17% were obtained for ten repeated measurements in the concentration of 10-9 M. Hence, the Raman results indicate that the active SERS substrates, gold nanoparticles for the detection of BPA along with the developed methods, show promising results for SERS-based studies and can lead to the development of microsensors. In Raman spectroscopy, SERS active substrate coated with gold nanoparticles are of interest, which is larger than gold particles due to the resonance of the surface plasmons of gold nanoparticles and the scattering of light from gold particles since the Raman signal amplifies the molecular vibrations of BPA. By decreasing the concentration of BPA deposited on the active SERS substrates, the Raman signal is also weakened due to the reduction of molecular vibrations. By increasing the surface roughness of the active SERS substrates, the Raman signal can be enhanced due to increased light scattering from rough centers, which are the same as the larger particles created throughout the deposition by the spin-coat method, and as a result, they enhance the signal by increasing the scattering of light. Then, the molecular vibrations of BPA were identified in some consumer goods by SERS substrates as plasmonic sensors and Raman spectroscopy.

Microwave assisted synthesis of gold nanoparticles and their antibacterial activity against <i>Escherichia coli</i> (<i>E. coli</i>)

Arshi, Nishat,Ahmed, Faheem,Kumar, Shalendra,Anwar, M.S.,Lu, Junqing,Koo, Bon Heun,Lee, Chan Gyu Elsevier 2011 Current Applied Physics Vol.11 No.1

<P><B>Abstract</B></P><P>We report a simple one step microwave irradiation method for the synthesis of gold nanoparticles using citric acid as reducing agent and cetyl trimethyl ammonium bromide (CTAB) as binding agent. The reaction was completed under two different microwave irradiation times (40 s and 70 s) for the production of two types of gold nanoparticles. The synthesized nanoparticles were characterized using UV–Vis absorption spectroscopy and transmission electron microscopy (TEM) measurements. UV–Vis study revealed the formation of gold nanoparticles with surface plasmon absorption maxima at 590 and 560 nm for 40 and 70 s irradiation time respectively. From TEM analysis, it is observed that the gold nanoparticles have spherical shape with particle size distribution in the range 1–10 nm and 1–2 nm for 40 s and 70 s irradiation time respectively. Antibacterial activity of gold nanoparticles as a function of particle concentration against gram-negative bacterium <I>Escherichia coli</I> (<I>E. coli</I>) was carried out in solid growth media. The two types of gold nanoparticles show high antibacterial activity with zone of inhibition of about 22 mm against <I>E. coli</I> (ATCC 25922 strain). Very small difference in the antibacterial activity for the two types of gold nanoparticles were observed. Though nanoparticles synthesized for 70 s irradiation time show slightly better antibacterial activity.</P> <P><B>Highlights</B></P><P>► Gold nanoparticles have been successfully synthesized using microwave irradiation technique. ► The mean diameter was ∼ 4.05 nm for 40 s and ∼1.05 nm for 70 s. ► Characteristic absorption peak was found to be 590 nm for 40 s and 560 nm for 70 s. ► The zone of inhibition for the two types of nanoparticles was almost similar (22 mm). ► Smaller size nanoparticles synthesized for 70s showed a slightly better antibacterial action.</P>

금으로 코팅된 산화철 나노입자에서의 표면 플라즈마 공명

입팔 유샤프,배홍섭,이일수,홍성욱 한국물리학회 2015 새물리 Vol.65 No.3

Gold-coated iron-oxide nanoparticles were synthesized by using chemical reactions. The X-Ray diffraction (XRD) confirmed the crystallinity of the cubic spinel structure. Transmission electron microscope (TEM) images showed that the gold-coated iron-oxide nanoparticles were spherical in shape with an average diameter of 15.5 nm. The binding of the gold to the surface of the ironoxide nanoparticles was revealed by using measurements made with Fourier transform infrared (FTIR) spectrometer. The vibrating sample magnetometer (VSM) results showed no hysteresis, indicating that the nanoparticles were superparamagnetic with a single domain. A surface plasma resonance was observed in an aqueous solution of the gold-coated iron-oxide nanoparticles, which provided direct evidence that the gold was well bound to the surfaces of the iron-oxide nanoparticles. The wavelength of the surface plasma resonance of the gold-coated iron-oxide nanoparticles was compared with those of gold nanoparticles. We observed a red-shifted surface plasma wavelength for the gold-coated iron-oxide nanoparticles, which was another piece of evidence for gold being well bound on the surfaces of the iron-oxide nanoparticles. 산화철 나노입자를 형성하고, 그 표면을 금으로 코팅하였다. TEM을 통해 관찰한 나노입자는 구형이었으며, 그 평균 직경이 15.5 nm 이었다. XRD패턴에서 산화철 나노입자는 입방 스피넬 (cubic spinel)의 결정성을 가지고 있음을 알 수 있었다. 코팅된 금의 나노입자 표면에의 부착상태는 FTIR을 이용하여 관찰하였다. 그리고 자기이력곡선에서 이력현상은 보이지 않았으며, 이는 나노입자는 단자구 (single domain)로서 초상자성을 가지고 있기 때문이다. 표면 플라즈마 공명현상을 관찰할 수 있었으며, 이는 금이 나노입자의 표면에 잘 부착되어 있음을 나타내는 또 다른 증거이다. 금으로 코팅된 산화철 나노입자의 표면 플라즈마 공명 파장을 금 나노입자의 공명 파장과 비교하였다. 이로부터 나노입자의 직경에 따라 나타나는 공명 파장의 적색편이 현상을 볼 수 있었다. 이는 금이 산화철 나노입자 표면에 잘 부착되었다는 것을 간접적으로 보여주는 결과이다.

Extracellular synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 and their biological applications

Singh, P.,Singh, H.,Kim, Y.J.,Mathiyalagan, R.,Wang, C.,Yang, D.C. IPC Science and Technology Press ; Elsevier Scienc 2016 Enzyme and microbial technology Vol.86 No.-

<P>The present-study highlights the microbial synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 strain, in an efficient way. The synthesized nanoparticles were characterized by ultraviolet visible spectrophotometry, which displayed maximum absorbance at 424 nm and 531 nm for silver and gold nanoparticles, respectively. The spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. The energy dispersive X-ray spectroscopy and elemental mapping were displayed the purity and maximum elemental distribution of silver and gold elements in the respective nanoproducts. The X-ray diffraction spectroscopy results demonstrate the crystalline nature of synthesized nanoparticles. The particle size analysis demonstrate the nanoparticles distribution with respect to intensity, volume and number of nanoparticles. For biological applications, the silver nanoparticles have been explored in terms of MIC and MBC against pathogenic microorganisms such as Vibrio parahaemolyticus, Escherichia coli, Salmonella enterica, Bacillus anthracis, Bacillus cereus and Staphylococcus aureus. Moreover, the silver nanoparticles in combination with commercial antibiotics, such as vancomycin, rifampicin, oleandomycin, penicillin G, novobiocin, and lincomycin have been explored for the enhancement of antibacterial activity and the obtained results showed that 3 mu g concentration of silver nanoparticles sufficiently enhance the antimicrobial efficacy of commercial antibiotics against pathogenic microorganism. Furthermore, the silver nanoparticles potential has been reconnoitered for the biofilm inhibition by S. aureus, Pseudomonas aeruginosa and E. coli and the results revealed sufficient activity at 6 mu g concentration. In addition, gold nanoparticles have been applied for catalytic activity, for the reduction of 4-nitrophenol to 4-aminophenol using sodium borohydride and positive results were attained. (C) 2016 Elsevier Inc. All rights reserved.</P>

Green Chemistry Approach for the Synthesis of Gold Nanoparticles Using the Fungus Alternaria sp

( Naresh Niranjan Dhanasekar ),( Ganga Ravindran Rahu ),( Kannan Badri Narayanan ),( Gurusamy Raman ),( Natarajan Sakthive ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7

The synthesis of gold nanoparticles has gained tremendous attention owing to their immense applications in the field of biomedical sciences. Although several chemical procedures are used for the synthesis of nanoparticles, the release of toxic and hazardous by-products restricts their use in biomedical applications. In the present investigation, gold nanoparticles were synthesized biologically using the culture filtrate of the filamentous fungus Alternaria sp. The culture filtrate of the fungus was exposed to three different concentrations of chloroaurate ions. In all cases, the gold ions were reduced to Au(0), leading to the formation of stable gold nanoparticles of variable sizes and shapes. UV-Vis spectroscopy analysis confirmed the formation of nanoparticles by reduction of Au3+ to Au0. TEM analysis revealed the presence of spherical, rod, square, pentagonal, and hexagonal morphologies for 1 mM chloroaurate solution. However, quasi-spherical and spherical nanoparticles/heart-like morphologies with size range of about 7-13 and 15-18 nm were observed for lower molar concentrations of 0.3 and 0.5 mM gold chloride solution, respectively. The XRD spectrum revealed the face-centered cubic crystals of synthesized gold nanoparticles. FT-IR spectroscopy analysis confirmed the presence of aromatic primary amines, and the additional SPR bands at 290 and 230 nm further suggested that the presence of amino acids such as tryptophan/tyrosine or phenylalanine acts as the capping agent on the synthesized mycogenic gold nanoparticles.

Sunlight-Induced Synthesis of Various Gold Nanoparticles and Their Heterogeneous Catalytic Properties on a Paper-Based Substrate

Kim, Jun-Hyun,Twaddle, Katrina M.,Hu, Jiayun,Byun, Hongsik American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.14

<P>This work describes the light-induced preparation of various gold nanoparticles and demonstrates their possible use as efficient photothermal heating materials and practical heterogeneous catalysts under the irradiation of a solar-based light after being loaded onto a paper-based substrate. The synthesis of gold nanoparticles was accomplished under the irradiation of daily sunlight and a solar-simulated light with an intensity that was closely adjusted to the one-sun condition. Tunable sizes of gold nanoparticles were systematically controlled by the ratio of trisodium citrate and gold chloride ions, particularly with the solar-simulated light source. The size distribution and absorption properties of the resulting nanoparticles were thoroughly characterized by scanning electron microscope, dynamic light scattering, and UV–visible spectroscopy. The broad-band solar-based light sources were found to be efficient external stimuli to induce/enhance the formation of various gold nanoparticles at room temperature. As gold nanoparticles typically exhibit efficient light-induced heating properties due to their strong absorption bands, these nanoparticles were physically embedded on a filter paper to examine their photothermal heating properties and heterogeneous catalytic activity in the reduction of 4-nitrophenol under the irradiation of the solar-simulated light. As expected, the gold-loaded filter papers exhibited a systematic increase of temperature as a function of the gold nanoparticle concentration and enhanced catalytic property under the irradiation of the light, presumably caused by the photothermally induced heating property of the loaded gold nanoparticles. Overall, solar-based light sources can offer dual functions for the synthesis and application of metal nanoparticles possessing strong absorption bands.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-14/am503745w/production/images/medium/am-2014-03745w_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am503745w'>ACS Electronic Supporting Info</A></P>

Plant Extracts Promoted Preparation of Silver and Gold Nanoparticles: A Systematic Review

Manobjyoti Bordoloi,Ranjan K. Sahoo,Kashyap J. Tamuli,Surovi Saikia,Partha P. Dutta 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.03

Eco-friendly synthesis of metal nanoparticles has accrued utmost interest by researchers in the last decade for their distinct properties making them applicable in different fields of science and technology. With regard to its low cost, low environmental effect, zero contamination and higher reducing potential, their synthesis by green chemistry procedure is an emerging area in nanobiotechnology. Plant-based nanoparticles produced are more stable, with high rate of synthesis and are suitable for large scale biosynthesis as compared to the use of microorganisms which require stringent control on cell cultures. Plant-based nanoparticles have advantages over other methods due to presence of biomolecules acting both as capping and reducing agents by increasing the rate of reduction and stabilization of nanoparticles. Furthermore, secondary metabolites present in plants are used for reducing metal ions in single step reaction. In this review paper, we have cited 265 research articles and have outlined 106 plant extract assisted gold and silver nanoparticles. The present review highlights the achievements of metal nanoparticle synthesis, especially silver and gold nanoparticles from plant extracts, along with factors liable for the synthesis of metal nanoparticles. It also focuses on the dye degrading properties and various biological activities of metal nanoparticles, their antimicrobial mechanism of action and the physicochemical properties that influence the biological effects of metallic nanoparticles. Biological activities of metal nanoparticles were also described, including the effect of physicochemical properties of metal nanoparticles on biological activities.

Use of Gold Nanoparticle Fertilizer Enhances the Ginsenoside Contents and Anti-Inflammatory Effects of Red Ginseng

( Hee Kang ),( Yun-gu Hwang ),( Taek-guen Lee ),( Cheng-ri Jin ),( Chi Heung Cho ),( Hee-yeong Jeong ),( Dae-ok Kim ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.10

Red ginseng, a steamed and sun-dried ginseng, is a popular health-promoting food in Korea and other Asian countries. We introduced nanofertilizer technology using gold nanoparticles in an effort to develop red ginseng with an elevated level of ginsenosides, the main active compounds of ginseng. Shoots of 6-year-old ginseng plants were fertilized three times with colloidal gold nanoparticle sprays. Red ginseng extract was prepared from the main roots. The concentrations of gold and ginsenosides were measured following gold nanoparticle treatment. To evaluate the anti-inflammatory effects, mouse peritoneal macrophages of male BALB/c mouse were stimulated with lipopolysaccharide plus interferon-γ in the presence of extracts from red ginseng with or without gold nanoparticle treatment. The content of ginsenosides, such as Rg1, Re, Rf, and Rb1, increased in ginseng treated with gold nanofertilizer whereas the steaming process increased only the levels of Rd and Rg3. The levels of nitric oxide, inducible nitric oxide synthase, and interleukin-6, but not tumor necrosis factor-α, were more suppressed in macrophages treated with extract from gold nanoparticle-treated red ginseng. Our results show that the use of a colloidal gold nanoparticle fertilizer improved the synthesis of ginsenosides in ginseng and enhanced the anti-inflammatory effects of red ginseng. Further research is required to elucidate the causal factors for the gold-induced change in ginsenoside synthesis and to determine the in vivo effect of gold nanoparticletreated ginseng.

Plasmonic effects and size relation of gold-platinum alloy nanoparticles

Jawad, Muhammad,Ali, Shazia,Waseem, Amir,Rabbani, Faiz,Amin, Bilal Ahmad Zafar,Bilal, Muhammad,Shaikh, Ahson J. Techno-Press 2019 Advances in nano research Vol.7 No.3

Plasmonic effects of gold and platinum alloy nanoparticles (Au-Pt NPs) and their comparison to size was studied. Various factors including ratios of gold and platinum salt, temperature, pH and time of addition of reducing agent were studied for their effect on particle size. The size of gold and platinum alloy nanoparticles increases with increasing concentration of Pt NPs. Temperature dependent synthesis of gold and platinum alloy nanoparticles shows decrease in size at higher temperature while at lower temperature agglomeration occurs. For pH dependent synthesis of Au-Pt nanoparticles, size was found to be increased by increase in pH from 4 to 10. Increasing the time of addition of reducing agent for synthesis of pure and gold-platinum alloy nanoparticles shows gradual increase in size as well as increase in heterogeneity of nanoparticles. The size and elemental analysis of Au-Pt nanoparticles were characterized by UV-Vis spectroscopy, XRD, SEM and EDX techniques.

Photothermal therapy with gold nanoparticles as an anticancer medication

김형식,이동윤 한국약제학회 2017 Journal of Pharmaceutical Investigation Vol.47 No.1

Over the past few decades, gold nanoparticles and diverse gold nano-forms have been considered for both cancer therapy and bioimaging due to their surface plasmon resonance effect and its capability of loading contrast agent. This effect enables thermal destruction of cells or organs by drastically elevating temperature when exposed to a specific energy of visible light either near infrared light. In addition, chemical modifications of the surfaces of gold nanoparticles or nano-forms are well known. Sulfurcontaining functional groups of biomolecules or polymers can be easily conjugated with Aurum atoms on the surface of gold nanoparticles. There are also various nano-forms with different shapes and sizes derived from gold nanoparticles such as the nanosphere, nanorod, nanocage, nanoshell, and nanoporous gold disks. The various forms of nano-scaled gold materials have unique properties that can be used as carrier, simultaneously thermal destruction agent. Its properties of the materials are able to applied to a number of desired purposes. Regardless of the physicochemical properties of gold nanoparticles, they present several challenges, such as the instance energy penetrating depth availability aspect required for gold nanoparticles to enter organs and the cellular toxicity issues of nano-scaled gold particles in the body. The purpose of this review is to introduce the advantages using gold nanoparticles-based materials and its diverse approaches to several types of cancer therapies due to its distinct properties.