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      • KCI등재

        Detection of Clostridium perfringens and its toxinotypes by enzyme linked immunosorbent assay from enterotoxaemic goats in Bangladesh

        Islam, K.B.M.S.,Rahman, M.S.,Ershaduzzaman, Md.,Taimur, M.J.F.A.,Jang, Hyung-Kwan,Song, Hee-Jong The Korean Society of Veterinary Service 2010 韓國家畜衛生學會誌 Vol.33 No.1

        An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.

      • KCI등재

        Detection of Clostridium perfringens and its toxinotypes by enzyme Linked immunosorbent assay from enterotoxaemic goats in Bangladesh

        ( K. B. M. S. Islam ),( M. S. Rahman ),( Md. Ershaduzzaman ),( M. J. F. A. Taimur ),( Hyung Kwan Jang ),( Hee Jong Song ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.1

        An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.

      • KCI등재

        Occurrence, clinical signs, postmortem lesions and etiology of enterotoxaemia in Black Bengal goats

        Islam, K.B.M. Saiful,Rahman, Md. Siddiqur,Ershaduzzaman, Md.,Taimur, M.J.F.A.,Song, Hee-Jong The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.4

        A year round study was carried out to investigate the etiology, clinical signs, postmortem lesions and occurrence of naturally occurring enterotoxaemia in Black Bengal goats. Sixteen goats of different age and sex died in different seasons with sigh associated with enterotoxaemia made the materials of this study. Accidental access to large amount of concentrate was noted as one of the predisposing factors although few cases were reported to occur without known diet change. Younger animals(50%) and males(62.50%) were found more prone to the disease and it was likely to be more prevalent during winter(50%) followed by at rainy season(31.25%) and summer(18.75%). Diarrhoea(81.25%), dullness(56.25%), drooping of the ears(50%), anorexia(43.75%) were recorded as major clinical signs whereas enterocolitis(100%), lung edema(87.50%), fluid filled intestines(87.50%), enlarged mesenteric lymph nodes(56.52%) etc. were most common post mortem lesions found. A few cases showed lesions on heart(31.25%), brain(25%) and/or liver/spleen(18.75%) but no lesion was found on kidney. Thus the so called 'pulpy kidney' lesion was absent. Intestinal contents were subjected to conventional bacteriological culture based methods to identify the causal agents. Based on the morphological, cultural and biochemical properties the causal agent was identified as Clostridium perfringens. Despite the study was carried out at certain area it showed a clear picture of goat enterotoxaemia in terms of etiology, clinical signs, postmortem lesions and occurrence of goat enterotoxaemia in Bangladesh.

      • KCI등재후보

        Occurrence, clinical signs, postmortem Lesions and etiology of enterotoxaemia in Black Bengal goats

        ( K. B. M. Saiful Islam ),( Md. Siddiqur Rahman ),( Md. Ershaduzzaman ),( M. J. F. A. Taimur ),( Hee Jong Song ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.4

        A year round study was carried out to investigate the etiology, clinical signs, postmortem lesions and occurrence of naturally occurring enterotoxaemia in Black Bengal goats. Sixteen goats of different age and sex died in different seasons with sigh associated with entero-toxaemia made the materials of this study. Accidental access to large amount of concentrate was noted as one of the predisposing factors although few cases were reported to occur without known diet change. Younger animals(50%) and males(62.50%) were found more prone to the disease and it was likely to be more prevalent during winter(50%) followed by at rainy season(31.25%) and summer(18.75%). Diarrhoea(81.25%), dullness(56.25%), drooping of the ears(50%), anorexia(43.75%) were recorded as major clinical signs whereas enterocolitis(100%), lung edema(87.50%), fluid filled intestines(87.50%), enlarged mesenteric lymph nodes(56.52%) etc. were most common post mortem lesions found. A few cases showed lesions on heart(31.25%), brain(25%) and/or liver/spleen(18.75%) but no lesion was found on kidney. Thus the so called ``pulpy kidney`` lesion was absent. Intestinal contents were subjected to conventional bacteriological culture based methods to identify the causal agents. Based on the morphological, cultural and biochemical properties the causal agent was identified as Clostridium perfringens. Despite the study was carried out at certain area it showed a clear picture of goat enterotoxaemia in terms of etiology, clinical signs, postmortem lesions and occurrence of goat enterotoxaemia in Bangladesh.

      • KCI등재

        Antibody Responses in Goats Vaccinated with a New Epsilon Toxoid Vaccine of Clostridium perfringens

        Rahmam, Mohammad Siddiqur,Rahmam, Mohammad Mostafizur,Baek, Byeong Kirl,Lee, John Hwa 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.1

        산양의 Clostridium균에 의한 장성중독증의 예방을 목적으로, Clostridium perfrigens의 독소로 제작된 새로운 epsilon toxoid를 예방백신으로서의 타당성을 증명하기 위해 이 toxoid에 대한 산양 내 항체생산능을 측정하였다. 본 실험에 사용된 산양은 총 20마리로서 이 toxoid 접종실험군 16마리와 대조군 4마리로 구분하였으며, 항체생산능 측정은 3주간 3회 실시하였다. 이 toxoid를 접종한 실험군에서는 접종 후 21일째 최고의 중화항체가를 보인 반면에 접종을 하지 않은 대조군에서는 항체가를 감지할 수 없었다. 실험군에서 접종 후 14일과 21일째 항체가 상승은 뚜렷한 유의성을 보였으며 (P<0.01), 이들 항체가는 또 높은 방어치(Protective level)를 나타냈다. 이 결과를 토대로 C. perfrigens로 제작된 새로운 이 epsilon toxoid는 산양의 Clostridium균에 의한 장성중독증을 효과적으로 예방할수 있을 것으로 사료된다.

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