How does a predator find its prey? Nesidiocoris tenuis is able to detect Tuta absoluta by HIPVs

Abdollahipour Mousa,Fathipour Yaghoub,Mollahosseini Afsaneh 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

The Zoophytophagous predator, Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae) is one of the most important candidates for controlling Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in tomato crops. This predator uses different signals including morphological plant traits, prey insects, and volatile substances produced by the infested plants and prey signals to find its suitable prey. These signals are different in each cultivar of a plant. We aimed to understand how N. tenuis finds its prey using volatiles from tomato plants damaged or infested with T. absoluta. The predator’s responses to various plant treatments on two cultivars of tomato plants were tested in a flight tunnel and a four-choice olfactometer. The volatile compounds released from the treatments were also collected and identified. The results of the olfactory experiments showed that the predators even in the absence of light chose the plants bearing their insect prey. This behavior was not the same in both cultivars, and N. tenuis had a tendency toward mechanically damaged of Early Urbana Y cultivar more than Cal JN3 cultivar. The differences in the amount of monoterpenes, sesquiterpenes, and eugenol between cultivars may play a role in the differential attraction of N. tenuis towards infested plants. The difference in the volatile compounds was evident in two cultivars, and this was consistent with our bioassay results. Therefore, the choice of appropriate cultivar and use of herbivore-infested plant volatiles are important for developing a control strategy against T. absoluta and attract its predators.

Sex pheromone of the jumping plant bug, Halticus minutus Reuter (Hemiptera: Miridae)

Tao Zhang,Xiaofang Zhang,Xiangdong Mei,Yaofa Li,Zhanlin Gao,Jun Ning 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

The jumping plant bug, Halticusminutus Reuter, is a pest of sweet potatoes, soybeans and turfgrass in China. Here we present our analysis of the female-produced sex pheromone. These plant bugs release their sex pheromone, consisting hexyl acetate (HA), hexyl butyrate (HB) and (E)-4-oxo-2-hexenal (4-OHE), at night. These three components, tested individually, elicited strong antennal responses. Field tests indicated that a ternary blend of HA, HB and 4-OHE, at ratios of 1:16:10, effectively attractedmale H. minutus. Our dose-response analysis showed optimal male capture at the trap dose of 13.5mg/trap. Higher trap doses did not improve capture rates. Higher ratios of HB in the pheromone mix led to substantially reduced captures, fromwhich we infer that HBmay function as an alarm pheromone or defensive secretion. Pipette tips were used as long-term lures, and whose longevities were evaluated. Results showed effective duration of pipette tip lures were N5 weeks. Thus, we suggest that the pheromone-baited traps are economically viable tools for long-term monitoring and trapping of jumping plant bugs.

논 담수 여부에 따른 유기농업자재의 먹노린재 방제 효과

이유경,최낙중,임주락,최준열,서보윤 한국환경생물학회 2023 환경생물 : 환경생물학회지 Vol.41 No.4

벼의 주요 해충인 먹노린재에 대해 실내에서 충체분무법과 기주분무법을 통해 시판 중인 유기농업자재 (EFOM) 27종을 대상으로 살충 활성을 평가하였다. 그 결과, 유기농업자재 7종 (EFOM-8, -10, -12, -13, -19, -20, -26)에서 기주식물에만 살포하는 것보다 충체에 직접 살포되어 접촉하는 경우에만 높은 살충 효과를 보였다. 실내 벼 포트 검정에서는 선발된 7종 모두 담수 조건에서 살충률이 73.3% 이상이었으며, 특히 EFOM-13과 EFOM-20은 퇴수 조건에서보다 담수 조건에서 살충률이 1.5~1.8배 높은 차이를 보였다. 야외 간이 검정에서 EFOM-10 (마늘 80%), EFOM-13 (님 62%), EFOM-26 (고삼 70%+에틸알콜 28%+제충국 2%)는 7일차 담수한 논에서 88.9%의 높은 방제가를 보였고 퇴수한 논보다 방제가는 1.4~1.9배 높았다. 선발된 EFOM-10을 살포한 농가의 담수한 논에서도 7일차 방제가는 86.2%로 퇴수한 논의 방제가 61.9%보다 1.4배 높았다. 종합적으로 살포액의 충분한 충체 접촉과 논의 담수 조건이 유기농업자재의 방제 효과를 높일 수 있음을 보여주었다. 이러한 연구 결과가 앞으로 농가에서 적용할 수 있는 최적의 먹노린재 방제전략 수립에 도움이 되기를 기대한다. The insecticidal activities of 27 different commercial products with environmentally friendly organic material (EFOM) against Scotinophara lurida, a major rice pest, were evaluated in the laboratory using spraying methods on plants and insects. Seven plant-derived organic farming materials (EFOM-8, -10, -12, -13, -19, -20, and -26) with high insecticidal effects when sprayed directly on the insect’s body rather than on the plant were selected. In the indoor rice pot test, all 7 EFOMs showed an insecticidal rate of over 73.3% under irrigated conditions. Notably, EFOM-13 and EFOM-20 demonstrated much higher insecticidal rates, ranging from 1.5 to 1.8 times, in irrigated conditions compared to drained conditions. In the semi-paddy field test, EFOM-10 (80% garlic extract), EFOM-13 (62% neem extract), and EFOM-26 (70% sophora extract+28% ethyl alcohol+2% pyrethrum extract) exhibited a higher control value of 88.9% in the irrigated paddy on the 7th day, surpassing the control values in the drained paddy by 1.4 to 1.9 times. The control value in the irrigated rice paddy field sprayed with EFOM-10 reached 86.2% on the 7th day, which was 1.4 times higher than 61.9% in the drained paddy. Taken together, the findings suggest that direct contact of the insect’s body with sufficient amounts of spray solution and the maintenance of paddy irrigation can enhance the controlling effect of EFOMs. These findings will be valuable in developing an optimal S. luridacontrol strategy for application in rice paddy fields in the near future.

노린재 흡즙이 차엽의 카테킨 및 아미노산 함량에 미치는 영향

송연상(Yeon-Sang Song),한선경(Seon-Kyeong Han),문윤호(Youn-Ho Moon),정병춘(Byeong-Choon Jeong),방진기(Jin-Ki Bang) 한국차학회 2008 한국차학회지 Vol.14 No.2

차나무 재배시 문제해충 중의 하나인 노린재의 흡즙피해를 받은 차엽의 기능성분 변화를 알아보기 위하여 실험한 결과를 요약하면 다음과 같다. 1. 피해엽의 수분함량, 탄닌함량, 총질소 함량은 정상엽에 비하여 낮은 수준을 나타내었다. 2. 카페인 함량은 차이가 미미하였으며 카테킨 함량은 피해엽이 정상엽에 비하여 훨씬 낮아졌다. 특히 EGCG의 함량변화가 크게 나타났다. 3. 아미노산함량 또한 피해엽이 정상엽보다 낮았는데 프로린과 페닐알라닌 함량에서 큰 차이를 보였다. 4. 차나무 계통 W2-1은 피해엽에서 정상엽보다 더 높은 아미노산 함량을 나타내었다. The true bugs are one of the problem insect decreasing tea quality by sucking to young leaves of tea plant. The purpose of this study was to understand the quality changes of tea leaves according to the sucking by true bugs. The damaged 8 accessions and the normal 8 accessions were selected on the tea germplasm garden and analyzed. Water content in damaged tea leaves showed lower than that of normal leaves. In case of average tannin content, it was observed slightly low in damaged leaves, whereas the normal leaves showed low tannin content among the same accessions. Total nitrogen, caffeine, and amino acid content, the important factors related to tea quality, showed highly in normal tea leaves compare to damaged tea leaves. It showed more difference between damaged and normal tea leaves in the same 3 accessions than that of among different 16 accessions. Therefore, it is important that we have to carefully control to insect in tea cultivation, because bugs sucking will be decrease tea quality. In addition, W2-1, accession name, showed high amino acid content in damaged tea leaves as 1,145 mg% compare to normal tea leaves with 973 mg%. It was considered that it is need to further study to understand the reason.

Survey of flowering plants in Hawaii as potential banker plants of anthocorid predators for thrips control

Frances Calver,Robert G. Hollingsworth,Marisa Wall,Peter A. Follett 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Flowering plants in gardens and along roadsides on the Big Island of Hawaii were sampled for thrips and anthocorid predators of thrips. A total of 171 plant samples, comprising 859 plant sample units (e.g. flowers or flower clusters) were collected from 56 species of plants in 25 families. Adult thrips were found on 43 plant species, and 32 of these also had larval thrips of the same species, indicating the plant species was a breeding host for thrips. Five different species of anthocorids – Orius persequens, Orius tristicolor, Paratriphleps laeviusculus, Montandoniola confusa, and Blaptostethus pallescens – were collected on 22 different plant species in 10 plant families. The plants with the highest numbers of anthocorid adults and nymphs present were Macaranga tanarius (Blush Macaraga), Verbesina encelioides (Golden Crownbeard), Tithonia diversifolia (Tree Marigold), Acalypha hispida (Chenille bush), and Coreopsis lanceolata (Lance-leaf Coreopsis). Macaranga tanarius was found to be the best host plant for anthocorids, with an average of 25.5 adult and 21.1 larval anthocorids per plant sample. Orius persequens was the most abundant anthocorid on M. tanarius with average adult and larval densities of 24.1 and 17.3 per plant sample, respectively. None of the insects found in association with M. tanarius are known pests. Macaranga tanarius has great potential as a banker plant to help suppress thrips populations in greenhouse crops with anthocorid predators.

Herbivory by Strongylocoris leucocephalus (Hemiptera: Miridae) on a novel host plant Adenophora triphylla var. japonica in Japan

Etsuro Takagi 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.3

When monophagous and oligophagous insect herbivores colonize new areas, they sometimes use novel hosts. The availability of the new hosts can facilitate further expansion into regions beyond the geographic range ofthe original hosts, resulting in specialization on different host plant taxa in different parts of the herbivore'sgeographic range. Strongylocoris leucocephalus Linnaeus (Hemiptera: Miridae), which is a plant bug attackingCampanula spp., is widely distributed in the Eurasian continent, North Africa, and northern Japan. In the processof geographic range expansion, S. leucocephalus may incorporate novel host plants into their host range. I investigatedthe host plant species of S. leucocephalus in central Japan. Field observations revealed that the plant bugfed on the novel host plant Adenophora triphylla (Thunb.) A. DC. var. japonica (Regel) H. Hara (Campanulaceae),although feeding by S. leucocephalus was not associated with plant mortality. However, S. leucocephalus never fedon Campanula punctata Lam. var. hondoensis (Kitam.) Ohwi, the only Campanula species at the study site. Theplant bug S. leucocephalus and the novel host plant A. triphylla var. japonica are not entirely distributed sympatrically,suggesting that the plant bug has expanded its geographic range by incorporating new hosts.

잡곡유전자원을 이용한 유기종자생산 포장의 해충발생 소장

김정순,고호철,윤성탁,조양희,김정곤,심창기 한국작물학회 2010 Korean journal of crop science Vol.55 No.1

잡곡유기종자 안정적인 생산을 위해 병해충 관리의 기본이 되는 잡곡의 중요 병해충발생 소장 및 발생시기와 밀도를 조사하고자 본 연구를 실시하였다. 1. 2008년 조, 기장, 수수의 유기잡곡종자 생산 포장에서 조사기간 동안 조명나방의 발생 밀도는 수수자원의 밀도가 가장 높았으며 조자원에서 가장 낮은 발생 밀도를 보였으며, 2009년도 조명나방의 발생 밀도는 수수재배 포장의 밀도가 가장 높았으며 조재배 포장에서 가장 낮은 발생 밀도를 보였다. 2. 2008년 조명나방에 의한 잡곡의 피해주율을 조사하였더니, 지속적으로 증가하여 10월 16일에는 조 (60%), 수수 (47.5%), 기장 (43.8%) 순으로 높게 조사되었다. 2009년에는 6월 8일부터 9월 4일까지 지속적으로 증가하여 9월 4일에는 조 (51.3%), 수수 (42.5%), 기장(32.5%) 순으로 높게 나타났으며 기장자원은 6월 8일부터 6월 26일 사이에 그 피해가 발생하지 않았다. 3. 멸강나방 성충의 발생 시기나 밀도를 조사하였으나, 2008년, 2009년 모두 전혀 유인되지 않았으나 멸강나방 유충에 의한 피해는 지속적으로 관찰되었으며, 각자원별로 피해주율을 살펴보면, 조, 수수, 기장 순으로나타났다. 4. 2008년, 2009년 모두 멸강나방 유충에 의한 피해주율은 조, 수수, 기장 순으로 높게 나타났으며, 2008년 조자원에 대한 피해주율은 11.25~35.0%로 지속적으로 증가하였고, 수수와 기장의 피해주율은 각각 3.8~26.3%, 7.5~22.5%의 변이를 보였다. 2009년 각 자원별로 평균 피해주율은 조 자원이 가장 높게 나타났으며, 조는 2.5~36.3%, 수수는 0~25.0%, 기장은 0~21.3% 순으로 나타났다 5. 잡곡포장에서의 노린재 발생은2008년 풀색노린재, 알락수염노린재, 썩덩나무노린재, 3종류가 발생하였으며, 조와 기장 자원에서 풀색노린재의 발생 밀도가 가장 높았고, 2009년은 4종 (풀색노린재, 알락수염노린재, 썩덩나무노린재, 톱다리노린재)의 노린재가 발생하였고 조와 기장 자원은 풀색노린재와 톱다리노린재가 우점종 이었으며 알락수염노린재의 발생밀도는 낮게 나타났다. 수수 자원은 썩덩나무노린재와 톱다리노린재가 우점종 이었으며 알락수염노린재와 풀색 노린재는 낮은 밀도를 나타내었다. This study was carried out to screen the occurrence of insect pest on the organic seed producing field of minor grain germplasms, Pearl millet (Setaria italic L.), Sorghum (Sorghum bicolor L.), and Common millet (Panicum miliaceum L.) in Gangwon-do from 2008 to 2009. The artificial pheromone traps successfully attracted an Oriental corn borer, Ostrinia furnacalis (Guenee) on Pearl millet, but the Army worm, Pseudaletia separata, was not attracted in all of the minor grain fields. The ratio of damaged plants of Oriental corn borer and Army worm were appeared in order with Pearl millet, Common millet, and Sorghum. The morphological diversities of plant bug were shown as four kinds of species, Eastern green stinkbug (Nezara antennata), Brown-marmorated stinkbug (Halyomorpha halys), Sloe bug (Dolycoris baccarum), and Bean bug (Riptortus clavatus), on the organic seed producing field of minor grain. The average occurrence density of Eastern green stinkbug was the highest level in the three kind of minor grains, Pearl millet, Common millet, and Sorghum in 2008 and 2009. The dominant species are the Eastern green stinkbug and the Bean bug in Pearl millet and Common millet. The Sloe bug and the Bean bug possessed the highest population density in Sorghum

Ovicidal effect of plant extract mixture against seven major insect pests

Hee-A Lee,Young Su Lee,So-Hee Kim,Jong Yoon Choi,Ju-Hyung Yoo,Hyun Ju Lee 한국응용곤충학회 2024 한국응용곤충학회 학술대회논문집 Vol.2024 No.04

The ovicidal effect of plant extract mixture (5% cinnamon extract + 10% citronella oil + 30% citrus oil + 10% derris extract + 20% neem extract + 25% penetrating surfactant) against several major insect pests was tested using the spraying method. In the case of stink bugs, eggs tended to die during hatching. When treated with a plant extract mixture (500-times solution), mortality for Halyomorpha halys, Riptortus clavatus, Eurydema dominulus, Trialeurodes vaprarorium, Bemisia tabaci, Spodoptera exigua, and Agrotis ipsilon reached as high as 100%. Therefore, it is believed that industrialization will be feasible in the future.

Life Cycle and Descriptions of Nymphal Stages of Nesidiocoris tenuis (Reuter, 1895) (Hemiptera: Heteroptera: Miridae: Bryocorinae)

Hodan Lee,Junggon Kim,Sunghoon Jung 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

The plant bug, Nesidiocoris tenuis (Reuter, 1895), is used as a biological control agent on various crops such as tomato and paprika. In this study, the egg and all the nymphal stages of N. tenuis are described and illustrated for the first time based on morphological characters. The main morphological characters of each instar are as follows: body shape, body size, development of wing pads, density of pubescent covered with abdomen, and colorations of antenna and legs, etc. Additionally, biological information such as feeding habits and mating are also provided with life cycle photographs.

Molecular phylogeny of the plant bugs (Hemiptera: Miridae), based on mitochondrial and nuclear genes

Sung Hoon Jung,Ram Keshari Duwal,Seunghwan Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

The first comprehensive cladistic analysis of Miridae, the plant bugs, based on ~3000 base pairs of mitochondrial (COI, 16S) and nuclear (18S, 28SD3) genes, is presented. For 105 taxa (101 Mirid species in 7 subfamilies and 11 tribes, and 4 outgroups) in 7 subfamilies and 11 tribes of Miridae, the combined dataset was analyzed using ML (maximum likelihood), BC (bayesian criteria) and MP (maximum parsimony). Clades recovered in all analyses, comprise 6 main groups: Cimicomorpha and Miridae; Phylinae; Mirinae (Eurystylus + Polymerus + Proboscidocoris + Taylorilygus + Stenodemini); Orthotylinae; Mirini (Adelphocoris group except “Apolygus complex”); Apolygus complex; Deraecorinae; Bryocorinae + Isometopinae + Cylapinae. Our results indicate 1) the monophyletic relationships of two subfamilies Phylinae and Deraeocorinae within the family Miridae; 2) the paraphyly of subfamily Mirinae. Phylogenetic relationships at the tribal or subfamily level are provided in comparison with the prior studies based on morphological data.