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Shin, Jin Soo,Do Hwang, Seon,Kim, Hyun Soo,Cho, Sung Whan,Seo, Sang Heui Mary Ann Liebert, Inc ; Mary Ann Liebert, Inc ; OC 2010 VIRAL IMMUNOLOGY Vol.23 No.4
<P>Abstract Swine-origin pandemic 2009 A (H1N1) influenza viruses are still infecting humans, and humans are currently being vaccinated with the inactivated vaccine of 2009 A (H1N1) influenza virus. We wanted to determine the efficacy of 2009 A (H1N1) inactivated vaccine in ferrets. Ferrets immunized with one dose (7.5 mug) of 2009 A (H1N1) inactivated vaccine were not protected from infections of either pandemic H1N1 or seasonal H1N1 influenza viruses, while ferrets immunized with two doses of 2009 A (H1N1) inactivated vaccine were protected from infections of pandemic H1N1, but not seasonal H1N1 influenza viruses. IgG subtype of antibody was dominantly detected in tissues of immunized ferrets. Our study suggests that pandemic H1N1 vaccine may not elicit the antibody cross-reactive to the seasonal H1N1 influenza virus.</P>
Shin, Jin Soo,Kim, Hyun Soo,Cho, Sung Hwan,Seo, Sang Heui Mary Ann Liebert, Inc ; Mary Ann Liebert, Inc ; OC 2010 VIRAL IMMUNOLOGY Vol.23 No.3
<P>Outbreaks of highly pathogenic H5N1 influenza viruses have been reported in many countries worldwide. The possibility of pandemics caused by H5N1 influenza viruses is high since human infections by H5N1 viruses continually occur. In this study we determined the immune response and efficacy of inactivated H5N1 vaccine developed by reverse genetics in ferrets. Ferrets intramuscularly inoculated with two doses of H5N1 vaccine survived the lethal challenge with homologous or heterologous H5N1 influenza viruses, while 75% and 25% of ferrets immunized with one dose of H5N1 vaccine survived the lethal challenge with homologous and heterologous H5N1 influenza viruses, respectively. When we determined antibody subtypes specific for H5N1 influenza viruses in tissues and sera of vaccinated ferrets, IgG antibodies were detected mainly in the trachea, nostril, lung, heart, liver, kidney, intestine, spleen, and serum. Our results suggest that IgG antibodies may play a major role in protecting ferrets immunized with the inactivated H5N1 vaccine from lethal challenge with H5N1 influenza viruses.</P>
Ock, Sun-A,Jeon, Byeong-Gyun,Rho, Gyu-Jin Mary Ann Liebert 2010 Tissue engineering. Part C, Methods Vol.16 No.6
<P>Mesenchymal stem cells (MSCs) offer a great promise for regenerative medicine. Present study compared the characterization of porcine MSCs (pMSCs) derived from bone marrow extract with adult ear and fetal skin-derived cells on morphology, cell growth, alkaline phosphatase activity, proliferation ability, expression of cluster of differentiation (CD) markers (CD29, 45, and 90), cell cycle, protein and mRNA levels of Oct-4, Sox-2, and Nanog, and lineage differentiation ability. Skin-derived cells exhibited alkaline phosphatase activity and differentiation ability like pMSCs. pMSCs possessed a longer doubling time than skin-derived cells, and there was no difference in the ratio of G0/G1 phase between pMSCs and skin-derived cells. Except for CD29 and 90, all cells were found negative for CD45. Protein and mRNA expression of Oct-4, Sox-2 and Nanog were observed with similar intensity in all cells. Taken together, pMSCs and skin-derived cells revealed similar characteristics, and suggested the possible supportive role of skin-derived cells with MSCs for the regeneration of damaged tissues in cell-based therapies.</P>
Development of a <sup>177</sup>Lu-Labeled RGD Derivative for Targeting Angiogenesis
Ju, Chang Hwan,Jeong, Jae Min,Lee, Yun-Sang,Kim, Young Joo,Lee, Byung Chul,Lee, Dong Soo,Chung, June-Key,Lee, Myung Chul,Jeong, Seo Young Mary Ann Liebert 2010 Cancer Biotherapy & Radiopharmaceuticals Vol.25 No.6
<P>Abstract Various Arg-Gly-Asp (RGD) derivatives have been labeled with various radioisotopes for targeting 관(v)관(3) integrin, which is expressed during angiogenesis in tumor. In this study, 2-(4'-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA-SCN) and its c(RGDyK) conjugate (NOTA-SCN-c(RGDyK)) were labeled with (177)Lu, which is a near ideal radionuclide for treating tumors because it emits therapeutic beta particles and gamma rays for monitoring. (177)Lu (250?Bq) was labeled with 50? NOTA-SCN-c(RGDyK) quantitatively. The specific activity of (177)Lu-NOTA-SCN-c(RGDyK) was 1.44??10(5) Ci/mol. Biodistribution study was performed in Balb/c mice xenografted with CT-26 (mouse colon cancer) cells. The highest uptake was found in kidneys (7.56%??0.71% ID/g at 1 hour), and tumor uptake was 1.70%??0.33% ID/g at 1 hour postinjection. Moderate tumor-to-blood (2.36??0.29) and tumor-to-muscle (2.06??0.40) ratios were observed. This study shows that (177)Lu-NOTA-SCN-c(RGDyK) is a potential therapeutic agent for angiogenic tumors, but special care is required to prevent kidney toxicity.</P>