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- 저자 : Zewei Cai (검색결과 1 건)
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Bacterial Cellulose Immobilized S. cerevisiae as Microbial Sensor for Rapid BOD Detection
Chenyu Zhao,Guangshu Wang,Mengtao Sun,Zewei Cai,Zichu Yin,Yurong Cai 한국섬유공학회 2021 Fibers and polymers Vol.22 No.5
As one of the most important parameters to characterize the organic pollution of water, biochemical oxygendemand (BOD) determined through a rapid, in situ and on line method is very attractive. In this paper, a new BOD biosensorhas been developed, which are composed of the ecofriendly precursor of Saccharomyces cerevisiae (S. cerevisiae) embeddedin bacterial cellulose (BC) matrix and the Ketjen Black (KB) modified glassy-carbon electrode. Furthermore, a doublemediatorsystem is constructed using potassium ferricyanide and menadione in reaction cell to transfer electron from themicrobe to electrode. The responses of glucose glutamic acid (GGA) standard solutions are amperomertrically measured withan applied potential of 0.25 V versus Hg/Hg2Cl2 in a three-electrode system. Under the optimum conditions, the (BC/S. cerevisiae-menadione)/KB modified electrode shows a high operational stability with relative standard deviation (RSD) of4.16 % (fourteen assays), a good repeatability (RSD=3.10 %), a fast response time (in 20 minutes) and a wide linear range(from 10-220 mg O2 l-1). The BOD values measured by this method have been highly correlated with the standard BOD 5-daymethod for wastewater samples (R2=0.9859, n=3), indicating that it can meet the requirement of BOD rapid measurement.
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