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RISS 인기검색어
- 검색키워드
- 저자 : Zeinab Salehi (검색결과 3 건)
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Zeinab Salehi,Hamid Haghighat Ghahfarokhi,Abbas Ali Kodadadi,Rasoul Rahimnia 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.35 No.-
Magnetic nanoparticles (MNPs) were prepared by a co-precipitation method and thiol and ureafunctionalized using (3-mercaptopropyl) trimethoxysilane (MPTS) and 1-(3-trimethoxysilyl propyl)urea (TMSPU). The samples were characterized by SEM, XRD and FTIR, and BET surface areameasurement and used for immobilization of Candida rugosa lipase type VII (CRL7) and Thermomyceslanuginosus lipase (TLL) for a transesterification reaction. The loading capacities of the lipasesimmobilized on the functionalized MNPs were also studied. The activity and thermal stability of theimmobilized lipases were compared to those of the free enzymes too. The loading capacities of CRL7 andTLL on MNPs-TMSPU are 410 and 440 mg/g-MNPs, 5.9 and 5.5 times higher than those on theunfunctionalized MNPs, respectively. The initial activities of both CRL7 and TLL on MNPs-TMSPU arehigher than those on MNPs-MPTS. After 30 min incubation at 80 8C, the remained activity of TLL onMNPs-TMSPU is 2.5 times higher than that of the free enzyme. The remained activities of thebiocomposites assayed after each use in transesterification reaction demonstrate that TLL-MNPs-TMSPUbiocomposite has a high yield of 92%. The immobilized TLL on MNPs-TMSPU and MPTS retain 80% and70% of their initial activities after 8 times reuse, respectively. The functionalized MNPs are magneticallyseparated for reuse.
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Khadijeh Sherafatmand,Shohreh Fatemi,Zeinab Salehi 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.30 No.-
Catalytic activity of the whole cell baker’s yeast (Saccharomyces cerevisiae) was studied for convertingacetaldehyde (AC) to ethanol (ET) in a gas-phase packed bed reactor. The baker’s yeast was considered atfree and immobilized conditions, to convert AC as an air pollutant to the less harmful product of ET. Theinfluence of operating conditions, such as inlet AC concentration, cells’ water content and temperature,was studied on the cells’ activity. The best conditions from the point of ET productivity and cell stabilitywas detected at temperature of 318 K and water content of 0.23 0.02 g water/g dry cell, using free cells. The cell immobilization was performed by doping on the surface of multiwalled carbon nanotube (CNT)under a mild centrifugal force. The activity of CNT-immobilized cells towards ET formation was improved by6% and the stability of the cells was improved by 43% respectively in comparison with the free cells’ activityand stability.
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Delsuz Rezaee,Mojgan Bandehpour,Bahram Kazemi,Sara Hosseini,Zeinab Dehghan,Saiyad Bastaminejad,Mohammad Salehi The Korean Society for Reproductive Medicine 2022 Clinical and Experimental Reproductive Medicine Vol.49 No.4
Objective: This research investigated the effects of human chorionic gonadotropin (HCG)-producing peripheral blood mononuclear cells (PBMCs) on the implantation rate and embryo attachment in mice. Methods: In this experimental study, a DNA fragment of the HCG gene was cloned into an expression vector, which was transfected into PBMCs. The concentration of the produced HCG was measured using enzyme-linked immunosorbent assay. Embryo attachment was investigated on the co-cultured endometrial cells and PBMCs in vitro. As an in vivo experiment, intrauterine administration of PBMCs was done in plaque-positive female mice. Studied mice were distributed into five groups: control, embryo implantation dysfunction (EID), EID with produced HCG, EID with PBMCs, and EID with HCG-producing PBMCs. Uterine horns were excised to characterize the number of implantation sites and pregnancy rate on day 7.5 post-coitum. During an implantation window, the mRNA expression of genes was evaluated using real-time polymerase chain reaction. Results: DNA fragments were cloned between the BamHI and EcoRI sites in the vector. About 465 pg/mL of HCG was produced in the transfected PBMCs. The attachment rate, pregnancy rate, and the number of implantation sites were substantially higher in the HCG-producing PBMCs group than in the other groups. Significantly elevated expression of the target genes was observed in the EID with HCG-producing PBMCs group. Conclusion: Alterations in gene expression following the intrauterine injection of HCG-producing PBMCs, could be considered a possible cause of increased embryo attachment rate, pregnancy rate, and the number of implantation sites.
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