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Shengai Cui,Bing Zhu,Fuhai Li,Yuezhong Ye 대한토목학회 2016 KSCE Journal of Civil Engineering Vol.20 No.4
To study the bond performance between shotcrete and rock in a hot and humid tunnel, experiments on wet-sprayed shotcrete specimens under different hot and humid curing conditions (35°C, 50°C, 70°C, and standard curing conditions: 20°C with relative humidity 95%) were performed to examine the influence of thermal damage. The results show that the bond strength at 35°C is higher compared with that at standard curing conditions, but decreases when the temperature rises (especially at 70°C), indicating the adverse effects of thermal damage on bond strength. Based on these results, we used the worst hot and humid condition (70°C) as a case study to explore measures to improve bond strength under hot and humid conditions by adding mineral admixture or fiber material to the shotcrete mix. The experimental results suggest that adding fly ash or slag powder can enhance the bond strength, with the best effect achieved by adding fly ash.
Wei Hu,Jing Wang,Ian McHardy,Renate Lux,Zhe Yang,Yuezhong Li,Wenyuan Shi 한국미생물학회 2012 The journal of microbiology Vol.50 No.2
Exopolysaccharide (EPS) of Myxococcus xanthus is a wellregulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.