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Aging process is accompanied by increase of transglutaminase C
Park, Jeong Soo,Kwak, Sahng June,Choi, Jee Young,Yeo, Eui Ju,Kim, Kyung Ok,Seong, Sang Cheol,Hwang, Yong Chul,Park, Sang Chul,Park, Yong Hoon,Han, Jeong A 한림대학교 한림과학원 부설 환경ㆍ생명과학연구소 1998 국제학술회의 Vol.1998 No.-
Crosslinking has been suggested as one of the mechanisms involved in the aging process. Among the various random or enzyme-mediated crosslinking reactions, transglutaminase (TGase)-catalyzed crosslinking activity has been proposed for its possible involvement in cell proliferation, differentiation, carcinogenesis, programmed death and aging. Moreover, recent findings of TGase C as a putative signal transducer and cell cycle regulator has renewed interest in the study of TGase C in relation to aging phenomena. The ubiquitous presence of TGase C compared to the organ-specific localization of other types of TGases has attracted special attention as a cellular aging device. In the present study, for in vitro studies we have analyzed the comparative pattern of TGase C in young and old human red blood cells, separated by density defferentiation, and in early and late-passage of hydrogen peroxide-treated human primary fibroblasts. For in vivo study, we monitored the age-dependent changes of TGase C in the liver and brain tissues of 4, 12, 18, and 24-month-old Sprague-Dawley rats. We obtained evidence that both the activity and protein levels of TGase C were high in old RBC and late-passage of hydrogen peroxide-treated vibroblasts. Similar findings were seen in liver and brain tissue such as age-dependent increases in TGase activity and protein level in an organ-specific pattern. These data suggest that TGase C might play an active role in the cellular process with age.
Park, Sang Eon,Kim, Hojeong,Lee, Jeongmin,Lee, Na Kyung,Hwang, Jung Won,Yang, Jin-ju,Ye, Byoung Seok,Cho, Hanna,Kim, Hee Jin,Kim, Yeo Jin,Jung, Na-Yeon,Son, Tae Ok,Cho, Eun Bin,Jang, Hyemin,Jang, Eun Cambridge University Press 2016 INTERNATIONAL PSYCHOGERIATRICS - Vol.28 No.1
<B>ABSTRACT</B><B>Background:</B><P>Decreased hemoglobin levels increase the risk of developing dementia among the elderly. However, the underlying mechanisms that link decreased hemoglobin levels to incident dementia still remain unclear, possibly due to the fact that few studies have reported on the relationship between low hemoglobin levels and neuroimaging markers. We, therefore, investigated the relationships between decreased hemoglobin levels, cerebral small-vessel disease (CSVD), and cortical atrophy in cognitively healthy women and men.</P><B>Methods:</B><P>Cognitively normal women (<I>n</I> = 1,022) and men (<I>n</I> = 1,018) who underwent medical check-ups and magnetic resonance imaging (MRI) were enrolled at a health promotion center. We measured hemoglobin levels, white matter hyperintensities (WMH) scales, lacunes, and microbleeds. Cortical thickness was automatically measured using surface based methods. Multivariate regression analyses were performed after controlling for possible confounders.</P><B>Results:</B><P>Decreased hemoglobin levels were not associated with the presence of WMH, lacunes, or microbleeds in women and men. Among women, decreased hemoglobin levels were associated with decreased cortical thickness in the frontal (Estimates, 95% confidence interval, −0.007, (−0.013, −0.001)), temporal (−0.010, (−0.018, −0.002)), parietal (−0.009, (−0.015, −0.003)), and occipital regions (−0.011, (−0.019, −0.003)). Among men, however, no associations were observed between hemoglobin levels and cortical thickness.</P><B>Conclusion:</B><P>Our findings suggested that decreased hemoglobin levels affected cortical atrophy, but not increased CSVD, among women, although the association is modest. Given the paucity of modifiable risk factors for age-related cognitive decline, our results have important public health implications.</P>
( Yeo-ok Park ),( Hee-jeong Jae ),( Ji-young Shon ),( Seong-tae Choi ),( Sung-chul Kim ),( Yong-cho Cho ),( Kwang-pyo Hong ),( Younghoon Park ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.3
Persimmon (Diospyros kaki Thunb.) is classified into four types based on the fruit traits, astringency and flesh color. Of the four types, the pollination-constant non-astringent (PCNA) fruit is typically most desirable for consumption. In the present study, we used five sequence characterized amplified region (SCAR) markers associated with astringency in persimmon fruit, namely E4/E9r, E4/A2r, 7H9F/AST-R, AST-F/AST-R, and AST-F/PCNA-F/5R3R, to improve the efficiency of PCNA-type persimmon breeding via marker-assisted selection (MAS). A total of 84 cultivars of the four types and their segregating F1 progeny were used to evaluate the association of SCAR markers with the fruit astringency phenotype. Polymerase chain reaction evaluation of each SCAR marker showed that E4/E9r combined with AST-F/PCNA-F/5R3R was appropriate for selecting the ast allele responsible for PCNA-type fruit, as the phenotype-genotype match percentages of these two markers were 94% and 99%, respectively. This MAS was verified by the successful use of AST-F/PCNA-F/5R3R to select 107 PCNA-type individuals from 609 F1 hybrid progeny derived from various crosses.
Anti-inflammatory Effect of Flower Bud and Fruit of Sweet Persimmon, Diospyros kaki T.
Park, Yeo Ok,Lee, Jeong Ah,Park, Seong Moon,Ha, Min Hee,Joo, Woo Hong,Kim, Dong Wan 대한의생명과학회 2020 Biomedical Science Letters Vol.26 No.2
Various beneficial effects of sweet persimmon (Diospyros kaki T.) including anti-oxidation, anti-bacteria and viruses, anti-allergy were widely reported previously. However, the anti-inflammatory effect and its molecular mechanisms are not clear. In this study, the anti-inflammatory effect of the extracts of flower bud and fruit of sweet persimmon was investigated in LPS-treated RAW264.7 cells. Both extracts of flower bud and fruit showed strong inhibitory effect on the LPS-induced NF-κB activation. IκBα, the inhibitor of NF-κB, was increased and the expressions of NF-κB target genes, COX-2 and iNOS, were suppressed by the treatment with the extracts of flower bud and fruit. The expressions of pro-inflammatory cytokines, IL-1β, IL-6, TNF-α were also suppressed by the extracts. In addition, the LPS-induced wnt/β-catenin pathway and its related gene expressions including cyclin D1, wnt 3a, wnt 5a were suppressed by the extracts. The extracts also showed anti-oxidant activity and suppressive effect on the LPS-induced apoptosis of RAW264.7 cells. These results suggest that the flower bud and fruit of sweet persimmon display strong anti-inflammatory effect through inhibiting the pro-inflammatory signaling pathways in the cells.
단감 품종 판별을 위한 single nucleotide polymorphism 마커 적용 검정
박여옥(Yeo Ok Park),최성태(Seong-Tae Choi),손지영(Ji-Young Son),김은경(Eun-Gyeong Kim),안광환(Gwang-Hwan Ahn),박지혜(Ji Hae Park),정완규(Wan-Kyu Joung),장영호(Young Ho Jang),김동완(Dong Wan Kim) 한국생명과학회 2020 생명과학회지 Vol.30 No.7
최근 next-generation sequencing technology의 발달로 유전체 분석 사례는 증가하고 있으나, 단감에 있어 적용가능한 Single Nucleotide Polymorphism (SNP) 마커 및 적용 결과는 거의 없는 실정이다. 이에 우리나라 고유떫은감 5품종으로부터 개발된 SNP primer 들을 단감 품종에 적용하여 사용 가능성을 검증하고자 수행하였다. Jung 등에 의해 개발된 19개 SNP primer들의 PCR 조건을 확인 한 후 본 실험의 전기영동 방식으로는 분석이 매우 어려웠던 8개의 primer를 제외한 11개의 SNP primer들을 최종 선발하였다. 1, 2차 검증을 통해 최종 선발된 11개의 SNP primer 들을 76품종 및 계통(불완전단감 20, 완전단감 30, 완전떫은감 20, 불완전떫은감 6)에 적용한 결과 38품종 및 계통(불완전단감 8, 완전단감 18, 완전떫은감 9, 불완전떫은감 3품종)은 각 품종 및 계통 간 구분을 할 수가 없었다. 그러나 최종 선발된 11개의 SNP primer 들을 신품종에 적용한 결과만를 보면 ‘감누리’, ‘단누리’, ‘홍추’와 ‘자미시’, ‘미감조생’을 동시에 구분할 수 있어 단감 신품종 판별을 위한 특이적 마커로 사용될 수 있을 것으로 판단된다. The recent development of next-generation sequencing technology has enabled increased genomic analysis, but very few single nucleotide polymorphism (SNP) markers applicable to sweet persimmon (Diospyros kaki Thunb.) cultivars have been identified. In this study, SNP primers developed from five pollination-constant astringent (PCA) persimmons native to Korea were applied to discriminate between cultivars and verify their usability. The polymerase chain reactions of 19 SNP primers developed by Jung et al. were checked, with 11 primers finally selected. The other eight were very difficult to analyze in the agarose gel electrophoresis and QIAxcel Advanced System used in this experiment and were therefore excluded. The 11 SNP primers were applied through first and second verification to 76 cultivars and collection lines including 20 pollination-variant non-astringent (PVNA), 30 pollination- constant non-astringent (PCNA), 20 PCA, and six pollination-variant astringent (PVA). Of these, 38 were indistinguishable (eight PVNA, 18 PCNA, nine PCA, and three PVA). However, the results of applying the 11 SNP primers to new sweet persimmon cultivars, namely Gamnuri, Dannuri, Hongchoo, Jamisi, and Migamjosaeng, showed that they have the potential to be used as a unique marker for simultaneously determining between them.