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Yasuyuki Ishii,Kiichi Fukuyama,Takanori Iwakiri,Ahmad Wadi,Sachiko Idota 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
In southern Kyushu, dwarf variety of late-heading type (DL) napiergrass (Pennisetum purpureum Schumach) has utilized for the rotational grazing in 4 months of hot summer period by Japanese-Black (JB) beef cows for 6 years in 2002-2007. The objectives of this study were to investigate the establishment of feeding system of DL napiergrass with Italian ryegrass (Lolium multiflorum Lam., IR) grazed by the standard herd of JB beef cows in breeding beef farmers. Grazing DL napiergrass pasture was established totally at 0.66 ㏊ with 5 paddocks (0.132 ㏊ each) in 2006. Oversowing of IR was imposed into the inter-row of DL napiergrass on December 8, 2007. Grazing for IR and DL napiergrass pastures was carried out in 30 and 71 days, respectively. Daily gain of beef cows for the grazing on DL napiergrass was averaged at 0.23 ㎏ head?¹ day?¹, and carrying capacity was calculated at 891 CD ㏊?¹. The results revealed that DL napiergrass with IR pasture should fit to the rotational grazing with maintaining the liveweight of grazing beef cows for 4.5 months, and it can be proposed for the sustainable and low-cost feeding system to smallholder farmers in this area.
Yasuyuki Aoyagi,Yasushi Saito,Masayuki Kuroda,Sakiyo Asada,Hideaki Bujo,Shigeaki Tanaka,Shunichi Konno,Masami Tanio,Itsuko Ishii,Masayuki Aso 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.3
The development of clinically applicable scaffolds is important for the application of cell transplantation in various human diseases. The aims of this study are to evaluate fibrin glue in a novel protein replacement therapy using proliferative adipocytes and to develop a mouse model system to monitor the delivery of the transgene product into the blood and the fate of the transduced cells after transplantation. Proliferative adipocytes from mouse adipose tissue were transduced by a retroviral vector harboring the human lecithin-cholesterol acyltransferase (lcat) gene, and were subcutaneously transplanted into mice combined with fibrin glue. The lcat gene transduction efficiency and the subsequent secretion of the product in mouse adipocytes were enhanced using a protamine concentration of 500 μg/ml. Adipogenesis induction did not significantly affect the lcat gene-transduced cell survival after transplantation. Immunohistochemistry showed the ectopic enzyme production to persist for 28 days in the subcutaneously transplanted genetransduced adipocytes. The increased viability of transplanted cells with fibrin glue was accompanied with the decrease in apoptotic cell death. The immunodetectable serum LCAT levels in mice implanted with the fibrin glue were comparable with those observed in mice implanted with Matrigel, indicating that the transplanted lcat gene-transduced adipocytes survived and functioned in the transplanted spaces with fibrin glue as well as with Matrigel for 28 days. Thus,this in vivo system using fibrin is expected to serve as a good model to further improve the transplanted cell/scaffold conditions for the stable and durable cell-based replacement of defective proteins in patients with LCAT deficiency.
Aoyagi, Yasuyuki,Kuroda, Masayuki,Asada, Sakiyo,Bujo, Hideaki,Tanaka, Shigeaki,Konno, Shunichi,Tanio, Masami,Ishii, Itsuko,Aso, Masayuki,Saito, Yasushi Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.3
The development of clinically applicable scaffolds is important for the application of cell transplantation in various human diseases. The aims of this study are to evaluate fibrin glue in a novel protein replacement therapy using proliferative adipocytes and to develop a mouse model system to monitor the delivery of the transgene product into the blood and the fate of the transduced cells after transplantation. Proliferative adipocytes from mouse adipose tissue were transduced by a retroviral vector harboring the human lecithin- cholesterol acyltransferase ($lcat$) gene, and were subcutaneously transplanted into mice combined with fibrin glue. The $lcat$ gene transduction efficiency and the subsequent secretion of the product in mouse adipocytes were enhanced using a protamine concentration of $500{\mu}g/ml$. Adipogenesis induction did not significantly affect the $lcat$ gene-transduced cell survival after transplantation. Immunohistochemistry showed the ectopic enzyme production to persist for 28 days in the subcutaneously transplanted genetransduced adipocytes. The increased viability of transplanted cells with fibrin glue was accompanied with the decrease in apoptotic cell death. The immunodetectable serum LCAT levels in mice implanted with the fibrin glue were comparable with those observed in mice implanted with Matrigel, indicating that the transplanted $lcat$ gene-transduced adipocytes survived and functioned in the transplanted spaces with fibrin glue as well as with Matrigel for 28 days. Thus, this $in$ $vivo$ system using fibrin is expected to serve as a good model to further improve the transplanted cell/scaffold conditions for the stable and durable cell-based replacement of defective proteins in patients with LCAT deficiency.
강동진,Yasuyuki Ishii,Aya Nishiwaki 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2
In this study, the effects of shoot-cutting method on propagation using overwintering napiergrass (Pennisetum purpureum Schum.) buds were examined under soil conditions compared with plant growth regulators (PGRs) that are used as an effective method for increasing adventitious buds of several gramineous crops. To increase adventitious budding, we cut shoots of napiergrass at 2 weeks after planting and treated with the following four types of PGR in comparison with shoot-cutting: 4.44 mM BA as cytokinin, 3.63 mM triazine as anticytokinin, 5.37 mM NAA as auxin, and 2.22 mM TIBA as antiauxin, either by injection or direct spraying onto the buds. A large number of adventitious buds were observed in BA- and TIBA-injected buds at 4 WAT compared to shoot-cut or PGR-sprayed buds. However, an extremely low survival rate for tiller-divided plants (nursery plants) derived from both BA- and TIBA-injected plants was observed compared with non-PGR controls. Similar trends on the survival rate and relative tiller number in main shoot-cut plants derived from intact buds (without PGR treatment) were also observed in a repeat trial. The present study demonstrated that the method of shoot-cutting could contribute to increasing propagation efficiency for nursery plants compared with exogenous PGR application, and would be a practical method for increased nursery propagation of napiergrass under soil conditions.
Hadijah Hasyim,Yasuyuki Ishii,Sachiko Idota,Ahmad Wadi,Yasuhiro Sugimoto 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Storage and disposal of digested effiuent of manure (DEM) produced by biogas plant is a challenge to beef producers because of the mitigation of environmental pollution. The DEM contains essential plant nutrients and to use DEM as a soil amendment for crop production is a practical method to solve the disposal problem. In 2 years of cropping with dwarf napiergrass as a perennial summer grass and Italian ryegrass as an annual winter grass, DEM revealed to be so effective fertilizer as chemicals to increase dry matter yield and forage quality, especially crude protein (CP) content and in vitro dry matter digestibility (IVDMD). The efficient use of DEM can be an agronomic-and economic-viable management practice for sustainable crop production in Miyazaki, southern Kyushu of Japan.
Masahide Yasuda,Yasuyuki Ishii,Kazuyoshi Ohta 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6
Recently Napier grass (Pennisetum purpureumSchumach) has been recognized to meet the requirement oflignocellulosic bioethanol production, because it has lowlignin-content and a relatively high herbage mass per yearand per area. Therefore, pretreatment, saccharification, andfermentation processes for ethanol production from Napiergrass have been extensively studied. As pretreatmentmethod, acid, alkali, PBHW (pressurized batch hot water),and LMAA (low-moisture anhydrous ammonia) pretreatmentswere reviewed. As saccharification and fermentationprocess, saccharification followed by co-fermentation ofhexose and pentose, simultaneous saccharification andfermentation (SSF) followed by pentose fermentation,simultaneous saccharification and co-fermentation (SSCF)process were proposed. The SSCF was most advantageousprocess since the SSCF can prevent contamination risks ofother microorganism and can construct simple processingprocedure. An example of ethanol production from Napiergrass was a combination process of LMAA-pretreatmentwith SSCF which was performed for of LMAA-treatedNapier grass at 36°C for 96 h using cellulase, xylanase,Saccharomyces cerevisiae, and Escherichia coli KO11. The ethanol yield reached 74.1%. Thus, Napier grass wasthought to be a promising biomass for ethanol production.
Effect of Fertilizer with Low Levels of Potassium on Radiocesium-137 Decontamination
강동진,Hirofumi Tazoe,Yasuyuki Ishii,Katsunori Isobe,Masao Higo,Masatoshi Yamada 한국작물학회 2018 Journal of crop science and biotechnology Vol.21 No.2
Potassium (K) fertilizer closely affects both above-ground biomass production and radiocesium-137 (137Cs) uptake by plants from Cs-contaminated soil. Here we investigated the effects of low levels of K on aboveground biomass, 137Cs concentration, and 137Cs removal ratio (CR) using Napier grass (Pennisetum purpureum Schum.). Experimental plots fertilized with either 2 g K (20% K) or 6 g K (60% K) per m2 were compared with that with no K fertilization (0% K) under low-, medium-, and high-density planting conditions. As a result, with increasing amounts of K fertilizer, the aboveground biomass of Napier grass tended to increase, and the 137Cs concentration significantly decreased. The greatest CR was observed at the plot fertilized with 20% K and with plants grown under highly dense conditions. CR was positively correlated with biomass in all the experimental plots. Moreover, a positive correlation between CR and 137Cs concentration was found only at low levels of K application (0% and 20% K). In the present study, the application of 20% K produced more aboveground biomass and smaller decreases in 137Cs concentration in Napier grass compared with those with 0% K application; therefore, applying a fertilizer with low levels of K, such as 20% K, is better for 137Cs decontamination than applying 0% or 60% K fertilizer.
Renny Fatmyah Utamy,Yasuyuki Ishii,Ahmad Wadi,Sachiko Idota,Naoto Harada,Kiichi Fukuyama 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
In southern Kyushu, aged beef cow farmers hold limited available land and poor machinery equipment, while demand for herbage production increases rapidly due to high cost of imported herbage supply. Dwarf variety of late-heading type (DL) napiergrass (Pennisetum purpureum Schumach) meets the situation for smallholder farmers, especially in remote islands. The objectives of this study were to investigate adaptability and sustainability of DL napiergrass among 12 sites in southern Kyushu, by determine yield, quality and overwintering ability. The results revealed t㏊t DL napiergrass should adapt to the examined sites including isolated islands at an established year and produce satisfactory yield and quality herbage, if additional fertilizer application covers more t㏊n 10g N m?² year?¹ after establishment.