Edaphovirga cremea gen. nov., sp. nov., isolated from the rhizospheric soil of Codonopsis clematidea

Jin-Yan Xue,Meng-Yue Zhang,Yu Zhang,Juan Cheng,Li-Cheng Liu,Ying-Ying Wu,Tian-Yuan Zhang,Yi-xuan Zhang 한국미생물학회 2019 The journal of microbiology Vol.57 No.5

A Gram-negative, facultatively anaerobic, non-motile, nonspore- forming, coccoid or rod-shaped and creamy-pigmented bacterium, designated SYP-B2100T, was isolated from the rhizospheric soil of Codonopsis clematidea in the Xinjiang Uygur Autonomous Region, China. The optimal growth occurred at 28°C, pH 5.0, in the absence of NaCl. The cells tested positive in catalase and methyl red tests but negative in oxidase, urease, gelatinase, milk coagulation, and peptonisation, H2S production, nitrate reduction, and Voges-Proskauer tests. The major isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were C16:0 and summed feature 8. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The 16S rRNA gene sequence of strain SYP-B2100T was the most similar to that of Rahnella inusitata DSM 30078T (96.9%) within the family Enterobacteriaceae. The genomic DNA G+C content of strain SYP-B2100T was 50.3 mol%. The combined data from the phylogenetic, morphological, physiological, biochemical, and chemotaxonomic analyses presented in this study support the conclusion that strain SYP-B2100T represents a novel species of a new genus, for which the name Edaphovirga cremea gen. nov., sp. nov. is proposed; the type strain is SYPB2100T (= CGMCC 1.5857T = DSM 105170T = KCTC 62024T).

Comparison of Serum Tumor Associated Material (TAM) with Conventional Biomarkers in Cancer Patients

Shu, Jian,Li, Cheng-Guang,Liu, Yang-Chen,Yan, Xiao-Chun,Xu, Xu,Huang, Xin-En,Cao, Jie,Li, Ying,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Xiang, Jin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To compare expression level of serum tumor associated materials (TAM) with several conventional serum tumor biomarkers, eg., carcinoembryonic antigen (CEA), carbohydrate antigen19-9 (CA19-9), carbohydrate antigen 15-3 (CA15-3), alpha-fetoprotein(AFP), in selected solid tumors. Methods: Patients diagnosed histologically or cytologically with liver, breast, esophageal, gastric, colorectal or pancreatic cancers were enrolled into this study. After diagnosis, the level of TAM was determined by chemical colorimetry, and levels of conventional tumor markers was measured by chemiluminescence methods. Results: A total of 560 patients were enrolled into this study. No statistically significant difference was detected in TAM and the above mentioned tumor biomarkers in terms of their positivity and negativity ( P>0. 05). Conclusions: Detection of TAM in liver, breast, esophageal, gastric, colorectal, and pancreatic cancer patients demonstrates a good accordance with CEA, CA199, CA153, and AFP, thus suggesting that further study is warranted to verify whether TAM could be a surrogate for these conventional biomarkers.

Recycle of Crop Residues in Fields through Fermentation

Cheng-Long Yan,Xue-Feng Hu,Ming-Yang Cao,Hui-Hui Dai,Fan Luo 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Genetic Structure Among Populations of Elymus burchan-buddae and E. nutans (Triticeae; Poaceae) from the Qinghai-Tibetan Plateau

Xue-Bing Yan,Cheng-zhang Wang,Yu-Xia Guo,Bao-Rong Lu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Genetic structure of 22 populations of two self-pollinating pasture species, Elymus nutans and E. burchan-buddae collected from various altitudes of the Qinghai-Tibetan Plateau was studied using fluorescence-based amplified fragment length polymorphism technique. Analysis of molecular variance revealed 42.97% and 37.63% among-population variation for the two Elymus species, respectively, indicating that the majority of the total variation presented within populations. This result contradicts the common genetic variation pattern for a selfing plant species: lower generic variation within populations. STRUCTURE analyses of the Elymus populations indicated an evident admixture genetic structure, particularly among neighboring populations from the same region, supporting the hypothesis of considerable seed dispersal among populations. The among-population gene flow promulgated by grazing animals may promote the maintenance of genetic diversity in the pasture species, particularly in small and fragmented populations within a given region. The gene flow promulgated by grazing animals may promote the maintenance of genetic diversity in the pasture species, particularly in small and fragmented populations within a given region.

Attenuation of hyperoxic acute lung injury by Lycium barbarum polysaccharide via inhibiting NLRP3 inflammasome

Cheng-Ying Hong,Hua-Dong Zhang,Xue-Yan Liu,Yong Xu 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.10

Lycium barbarum polysaccharide (LBP), anactive component from Goji berry which is a traditionalChinese medicine, has anti-inflammatory and antioxidantfeatures. The aim of our study was to investigate whetherLBP has any role in hyperoxia-induced acute lung injury(ALI). Using a murine model of hyperoxia-induced ALI,we investigate the effect of LBP on pulmonary pathologicalchanges as well as Sirtuin 1 (SIRT1) and the nucleotidebinding domain and leucine-rich repeat pyrin domaincontaining 3 (NLRP3) inflammasome. Exposure to 100%oxygen for 72 h in male C57BL/6 mice resulted inincreased protein levels of tumor necrosis factor-a andinterleukin-1b in lung tissues, and aggravated lung histologicalalterations. These hyperoxia-induced changes andmortality were improved by LBP. LBP markedly suppressedthe activation of NLRP3 inflammasome bothin vivo and in vitro. Moreover, LBP upregulated SIRT1expression compared with vehicle-treated group. Importantly,knockdown of SIRT1 reversed the inhibitory effectof LBP on NLRP3 inflammasome activation in vitro. LBP meliorated hyperoxia-induced ALI in mice by SIRT1-dependentinhibition of NLRP3 inflammasome activation.

Treatment of oily wastewater from cold-rolling mill through coagulation and integrated membrane processes

Xue-Ni Cheng,Yan-Wen Gong 대한환경공학회 2018 Environmental Engineering Research Vol.23 No.2

The feasibility of applying coagulation-integrated microfiltration (MF) as a pretreatment for an ultrafiltration (UF) feed in oily wastewater treatment was investigated. The effects of different coagulants on oil removal rates from wastewater were studied. The maximum oil removal rate of 82% was obtained after coagulation with 130 mg/L of polyaluminium chloride (PAC). UF flux reached 95 L/(㎡·h) with coagulation-integrated MF as pretreatment. This value was 2.5 times higher than that flux obtained without pretreatment. The value of UF flux increased as the transmembrane pressure (TMP) and cross-flow velocity (CFV) of the UF module increased. UF flux gradually increased when TMP and CFV exceeded 0.4 MPa and 3 m/s, respectively, because of concentration polarization and membrane fouling stabilization. Chemical oxygen demand reduction and oil removal rate reached 95.2% and 98.5%, respectively, during integrated membrane processing with a PAC concentration of 130 mg/L, TMP of 0.4 MPa, and CFV of 3 m/s for UF. In addition, sequentially cleaning the fouling membrane with NaOH and HNO₃ aqueous solutions caused UF flux to recover to 90%. These encouraging results suggested that the hybrid integrated membrane process-based coagulation and MF + UF are effective approaches for oily wastewater treatment.

Further Education for Asian University Sports: AUSF Education and Development Centre

( Hao Cheng ),( Pei Liu ),( Jian Wang ),( Tieli Yang ),( Jun Xie ),( Bingshu Zhong ),( Qing Song ),( Cheng Fave Seetow ),( Yuetong Yan ),( Zhen Shen ),( Yanqing Xue ),( Kunning Chen ) 한국체육학회 2015 국제스포츠과학 학술대회 Vol.2015 No.1

Experimental study on Chinese ancient timber-frame building by shaking table test

Xi-cheng Zhang,Jian-yang Xue,Hong-tie Zhao,Yan Sui 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.40 No.4

A one-story, wooden-frame, intermediate-bay model with Dou-Gon designed according to the Building Standards of the Song Dynasty (A.D.960-1279), was tested on a unidirectional shaking table. The main objectives of this experimental study were to investigate the seismic performance of Chinese historic wooden structure under various base input intensities. El Centro wave (N-S), Taft wave and Lanzhou wave were selected as input excitations. 27 seismic geophones were instrumented to measure the real-time displacement, velocity and acceleration respectively. Dynamic characteristics, failure mode and hysteretic energy dissipation performance of the model are analyzed. Test results indicate that the nature period and damping ratio of the model increase with the increasing magnitude of earthquake excitation. The nature period of the model is within 0.5~0.6 s, the damping ratio is 3~4%. The maximum acceleration dynamic magnification factor is less than 1 and decreases as the input seismic power increases. The frictional slippage of Dou-Gon layers (corbel brackets) between beams and plates dissipates a certain amount of seismic energy, and so does the slippage between posts and plinths. The mortise-tenon joint of the timber frame dissipates most of the seismic energy. Therefore, it plays a significant part in shock absorption and isolation.

Longitudinal Intrinsic Brain Activity Changes in Cirrhotic Patients before and One Month after Liver Transplantation

Yue Cheng,Li-Xiang Huang,Li Zhang,Ming Ma,Shuang-Shuang Xie,Qian Ji,Xiao-Dong Zhang,Gao-Yan Zhang,Xue-Ning Zhang,Hong-Yan Ni,Wen Shen 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.2

Objective: To evaluate the spontaneous brain activity alterations in liver transplantation (LT) recipients using resting-state functional MRI. Materials and Methods: Twenty cirrhotic patients as transplant candidates and 25 healthy controls (HCs) were included in this study. All patients repeated the MRI study one month after LT. Amplitude of low-frequency fluctuation (ALFF) values were compared between cirrhotic patients (both pre- and post-LT) and HCs as well as between the pre- and post-LT groups. The relationship between ALFF changes and venous blood ammonia levels and neuropsychological tests were investigated using Pearson’s correlation analysis. Results: In the cirrhotic patients, decreased ALFF in the vision-related regions (left lingual gyrus and calcarine), sensorimotor-related regions (left postcentral gyrus and middle cingulate cortex), and the default-mode network (bilateral precuneus and left inferior parietal lobule) were restored, and the increased ALFF in the temporal and frontal lobe improved in the early period after LT. The ALFF decreases persisted in the right supplementary motor area, inferior parietal lobule, and calcarine. The ALFF changes in the right precuneus were negatively correlated with changes in number connection test-A scores (r = 0.507, p < 0.05). Conclusion: LT improved spontaneous brain activity and the results for associated cognition tests. However, decreased ALFF in some areas persisted, and new-onset abnormal ALFF were possible, indicating that complete cognitive function recovery may need more time.

Experimental study on Chinese ancient timber-frame building by shaking table test

Zhang, Xi-Cheng,Xue, Jian-Yang,Zhao, Hong-Tie,Sui, Yan Techno-Press 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.40 No.4

A one-story, wooden-frame, intermediate-bay model with Dou-Gon designed according to the Building Standards of the Song Dynasty (A.D.960-1279), was tested on a unidirectional shaking table. The main objectives of this experimental study were to investigate the seismic performance of Chinese historic wooden structure under various base input intensities. El Centro wave (N-S), Taft wave and Lanzhou wave were selected as input excitations. 27 seismic geophones were instrumented to measure the real-time displacement, velocity and acceleration respectively. Dynamic characteristics, failure mode and hysteretic energy dissipation performance of the model are analyzed. Test results indicate that the nature period and damping ratio of the model increase with the increasing magnitude of earthquake excitation. The nature period of the model is within 0.5~0.6 s, the damping ratio is 3~4%. The maximum acceleration dynamic magnification factor is less than 1 and decreases as the input seismic power increases. The frictional slippage of Dou-Gon layers (corbel brackets) between beams and plates dissipates a certain amount of seismic energy, and so does the slippage between posts and plinths. The mortise-tenon joint of the timber frame dissipates most of the seismic energy. Therefore, it plays a significant part in shock absorption and isolation.