Concomitant nitrates enhance clopidogrel response during dual anti-platelet therapy

Lee, D.H.,Kim, M.H.,Guo, L.Z.,De Jin, C.,Cho, Y.R.,Park, K.,Park, J.S.,Park, T.H.,Serebruany, V. Elsevier/North-Holland Biomedical Press 2016 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.203 No.-

<P>Background: Despite advances in modern anti-platelet strategies, clopidogrel still remains the cornerstone of dual anti-platelet therapy (DAPT) in patients undergoing percutaneous coronary interventions (PCI). There is some inconclusive evidence that response after clopidogrel may be impacted by concomitant medications, potentially affecting clinical outcomes. Sustained released nitrates (SRN) are commonly used together with clopidogrel in post-PCI setting for mild vasodilatation and nitric oxide-induced platelet inhibition. Methods: We prospectively enrolled 458 patients (64.5 +/- 9.6 years old, and 73.4% males) following PCI undergoing DAPT with clopidogrel and aspirin. Platelet reactivity was assessed by the VerifyNow (TM) P(2)Y12 assay at the maintenance outpatient setting. Results: Concomitant SRN (n = 266) significantly (p = 0.008) enhanced platelet inhibition after DAPT (251.6 +/- 80.9 PRU) when compared (232.1 +/- 73.5 PRU) to the SRN-free (n = 192) patients. Multivariate logistic regression analysis with the cut-off value of 253 PRU for defining heightened platelet reactivity confirmed independent correlation of more potent platelet inhibition during DAPT and use of SRN (Relative risk = 1.675; Odds ratio [1.059-2.648]; p = 0.027). In contrast, statins, calcium-channel blockers, beta blockers, angiotensin receptor blockers, ACE-inhibitors, diuretics, and anti-diabetic agents did not significantly impact platelet inhibition following DAPT. Conclusion: The synergic ability of SRN to enhance response during DAPT may have important clinical implications with regard to better cardiovascular protection, but extra bleeding risks, requiring further confirmation in a large randomized study. (C) 2015 Elsevier Ireland Ltd. All rights reserved.</P>

Effects of exogenous phytase and xylanase, individually or in combination, and pelleting on nutrient digestibility, available energy content of wheat and performance of growing pigs fed wheat-based diets

Yang, Y.Y.,Fan, Y.F.,Cao, Y.H.,Guo, P.P.,Dong, B.,Ma, Y. X. Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.1

Objective: Two experiments were conducted to determine the effects of adding exogenous phytase and xylanase, individually or in combination, as well as pelleting on nutrient digestibility, available energy content of wheat and the performance of growing pigs fed wheat-based diets. Methods: In Experiment 1, forty-eight barrows with an initial body weight of $35.9{\pm}0.6kg$ were randomly assigned to a $2{\times}4$ factorial experiment with the main effects being feed form (pellet vs meal) and enzyme supplementation (none, 10,000 U/kg phytase, 4,000 U/kg xylanase or 10,000 U/kg phytase plus 4,000 U/kg xylanase). The basal diet contained 97.8% wheat. Pigs were placed in metabolic cages for a 7-d adaptation period followed by a 5-d total collection of feces and urine. Nutrient digestibility and available energy content were determined. Experiment 2 was conducted to evaluate the effects of pelleting and enzymes on performance of wheat for growing pigs. In this experiment, 180 growing pigs ($35.2{\pm}9.0kg\;BW$) were allocated to 1 of 6 treatments according to a $2{\times}3$ factorial treatment arrangement with the main effects being feed form (meal vs pellet) and enzyme supplementation (0, 2,500 or 5,000 U/kg xylanase). Results: In Experiment 1, there were no interactions between feed form and enzyme supplementation. Pelleting reduced the digestibility of acid detergent fiber (ADF) by 6.4 percentage units (p<0.01), increased the digestibility of energy by 0.6 percentage units (p<0.05), and tended to improve the digestibility of crude protein by 0.5 percentage units (p = 0.07) compared with diets in mash form. The addition of phytase improved the digestibility of phosphorus (p<0.01) and calcium (p<0.01) by 6.9 and 7.6 percentage units respectively compared with control group. Adding xylanase tended to increase the digestibility of crude protein by 1.0 percentage units (p = 0.09) and increased the digestibility of neutral detergent fiber (NDF) (p<0.01) compared with control group. Supplementation of the xylanase-phytase combination improved the digestibility of phosphorus (p<0.01) but impaired NDF digestibility (p<0.05) compared with adding xylanase alone. In Experiment 2, adding xylanase increased average daily gain (p<0.01) and linearly improved the feed:gain ratio (p<0.01) compared with control group. Conclusion: Pelleting improved energy digestibility but decreased ADF digestibility. Adding xylanase increased crude protein digestibility and pig performance. Phytase increased the apparent total tract digestibility of phosphorus and calcium. The combination of phytase-xylanase supplementation impaired the effects of xylanase on NDF digestibility.

Impacts of Limestone Multi-particle Size on Production Performance, Egg Shell Quality, and Egg Quality in Laying Hens

Guo, X.Y.,Kim, I.H. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

This experiment was conducted to evaluate the effects of single or multi-particle size limestone on the egg shell quality, egg production, egg quality and feed intake in laying hens. A total of 280 laying hens (ISA brown) were used in this 10-wk trial. Laying hens were randomly assigned to 4 treatments with 14 replications per treatment and 5 adjacent cages as a replication (hens were caged individually). The experimental treatments were: i) L, basal diet+10% large particle limestone; ii) LS1, basal diet+8% large particle limestone+2% small particle limestone; iii) LS2, basal diet+6% large particle limestone+4% small particle limestone; iv) S, basal diet+10% small particle limestone. The egg production was unaffected by dietary treatments. The egg weight in S treatment was lighter than other treatments (p<0.05). The egg specific gravity in S treatment was lower than other treatments (p<0.05). The eggshell strength and eggshell thickness in S treatment were decreased when compared with other dietary treatments (p<0.05). The laying hens in LS1 and LS2 treatment had a higher average feed intake than the other two treatments (p<0.05). Collectively,the dietary multi-particle size limestone supplementation could be as efficient as large particle size limestone.

Measurement of the Intestinal Digestibility of Rumen Undegraded Protein Using Different Methods and Correlation Analysis

Y. Wang,Y.G. Zhang,Xiaolan Liu,N.K. Kopparapu,Hangshu Xin,J. Liu,Jianhua Guo 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.10

Four methods were adopted, including the mobile nylon bag (MNB) method, modified three-step in vitro (MTS) method, original three-step in vitro (OTS) method, and acid detergent insoluble nitrogen (ADIN) estimating method, to evaluate the intestinal digestibility of rumen undegradable protein (DRUP) of 10 types of concentrates and 7 types of roughages. After correlation analysis to determine the DRUP values using the MNB, MTS, OTS, and ADIN methods, the study aimed to find out appropriate methods to replace the MNB method due to its disadvantages such as high price, long time period, and use of a duodenal T-fistula. Three dairy cows with a permanent ruminal fistula and duodenal T-fistula were used in a single-factor experimental design. The results showed that the determined DRUP values using the MNB method for soybean meal, cottonseed meal, rapeseed meal, sunflower meal, corn germ meal, corn, rice bran, barley, wheat bran, corn fiber feed, Alfalfa (Zhao dong), Alfalfa (Long mu 801), Alfalfa (Long mu 803), grass (North), Grass (Inner Mongolia), corn silage and corn straw were 98.13%, 87.37%, 88.47%, 82.60%, 75.40%, 93.23%, 69.27%, 91.27%, 72.37%, 79.03%, 66.72%, 68.64%, 73.57%, 50.47%, 51.52%, 54.05%, and 43.84%, respectively. The coefficient of determination (R2 = 0.964) of the results between the MTS method and the MNB method was higher than that (R2 = 0.942) between the OTS method and the MNB method. The coefficient of determination of the DRUP values of the concentrates among the in vitro method (including the MTS and OTS methods) and the MNB method was higher than that of the roughage. There was a weak correlation between the determined DRUP values in concentrates obtained from the ADIN method and those from the MNB method, and there was a significant correlation (p<0.01) between the determined DRUP values of the roughage obtained from the MNB method and those obtained from ADIN method. The DRUP values were significantly correlated with the nutritional ingredients of the feeds. The regression equation was DRUP =100.5566+0.4169CP – 0.4344SP – 0.7102NDF – 0.7950EE (R2 = 0.8668, p<0.01; CP, crude protein; SP, soluble protein; NDF, neutral detergent fiber; EE, ether extract). It was concluded that both the MTS method and the OTS may suitable to replace the MNB method for determining the DRUP values and the former method was more effective. Only the ADIN method could be used to predict the values of the roughages but conventional nutritional ingredients were available for all of the samples’ DRUP.

Bioactive Natural Products from Chinese Tropical Marine Plants and Invertebrates

Guo, Y.W.,Huang, X.C.,Zhang, W.,Sun, Y.Q. The Korean Society for Marine Biotechnology 2006 한국해양바이오학회지 Vol.1 No.1

This paper deals mainly with chemical and biological investigations, recently effected in the authors' laboratory, of Chinese tropical marine plants and invertebrates on three topics: 1) an unusual macrocyclic polydisulfide from the Chinese mangrove Bruguiera gymnorrhiza; 2) polyhydroxylpolyene compounds from marine dinoflagellate Amphidinium sp.; 3) two new series of uncommon steroids from marine in vertebrates.

Groove Size Effect on CMP Characteristics and Analysis

Y. C. Guo(과용창),Y. K. Lee(이영균),H. S. Lee(이현섭),H. D. Jeong(정해도) 한국정밀공학회 2010 한국정밀공학회 학술발표대회 논문집 Vol.2010 No.11월

Bioactive Natural Products from Chinese Tropical Marine Plants and Invertebrates

Y.-W. Guo,X.-C. Huang,W. Zhang,Y.-Q. Sun 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.1

Early energy and lysine restriction (d 8 to 21) and realimentaion affected growth, blood profiles, and expression of genes related to protein and fat metabolism in broilers

Y. X. Yang(양위신),J. Guo,Z. Jin,S. Y. Yun,J. Y. Choi,X. S. Piao,S. J. Ohh,M. H. Wang,B. J. Chae 한국가금학회 2007 한국가금학회 정기총회 및 학술발표회 Vol.24 No.-

개시 후 8일령부터 2주간 라이신과 에너지 수준을 제한하였을 경우 육계의 성장과 보상성장에 미치는 영향에 대하여 조사하였다. 에너지와 라이신 제한 수준은 NRC(1994)를 기준으로 에너지 10 %, 라이신 20 %를 제한하였다. 사료내 에너지와 라이신 제한은 사양성적, 영양소 소화율, 소화장기 발달정도, 계육의 품질과 혈액의 생화학적 성상에 영향을 미치는 것으로 나타났다. Myostatin mRNA 함량은 라이신을 제한한 처리구에서 풍부한 것으로 나타났으나 복강지방 조직내 leptin mRNA는 에너지와 라이신 수준이 제한됨에 따라 유의적으로 감소하였다. 따라서 10% 에너지 제한은 성장, 사료효율과 계육 품질을 개선시키는 것으로 여겨진다. 또한 체단백질과 지방은 myostatin과 leptin mRNA 발현에 의해 조절되는 것으로 사료된다.

First principles study of nanoscale mechanism of oxygen adsorption on lanthanum zirconate surfaces

Guo, X.,Wu, L.,Zhang, Y.,Jung, Y.G.,Li, L.,Knapp, J.,Zhang, J. North-Holland 2016 Physica E, Low-dimensional systems & nanostructure Vol.83 No.-

<P>Lanthanum zirconate (La2Zr2O7) is a rare-earth pyrochlore material, which has been proposed as a promising thermal barrier coating (TBC) material due to its low thermal conductivity and high temperature phase stability. At elevated temperatures, degradation of La2Zr2O7 may occur due to adsorption of oxygen (02) on La2Zr2O7 surfaces. This paper investigates nanoscale mechanism of O-2 adsorption on La2Zr2O7 coating surfaces using the density functional theory (DFT) calculations. La2Zr2O7 surface energies on (001), (011) and (111) planes are calculated. The surface free energy of (011) plane is lower than those of (001) and (111) planes. On (001), (011) and (111) planes of La2Zr2O7, the lowest adsorption energy occurs at 4-fold site, bridge site, and 3-fold-FCC site, respectively. Among all calculated cases, the lowest adsorption energy site is 3-fold-FCC on (111) plane, which is confirmed by the Bader charge transfer analyses. Charge density difference analyses show that the 3-fold-FCC site on (111) surface has the largest charge density, suggesting the strongest interaction between O-2 and La2Zr2O7 surface. (C) 2016 Elsevier B.V. All rights reserved.</P>

Integrating Soil Information System with Agro-environmental Application in Taiwan

Guo, H.Y.,Hseu, Z.Y.,Chen, Z.S.,Tsai, C.C.,Jien, S.H. 한국토양비료학회 2009 한국토양비료학회 학술발표회 초록집 Vol.2009 No.10