http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Tong Shen,Jianming Li,Xiaoshun He,Meiling Lei,Jingru Wang,Xiuming Li 한국유전학회 2014 Genes & Genomics Vol.36 No.6
Here, the major histocompatibility complex(MHC) class II alpha chain gene in the Chinese longsnoutcatfish (Leiocassis longirostris) was characterized throughcloning, sequencing, polymorphism analysis, and expressionpatterns in various tissues. The full-length LeloDAAcDNA comprises 1,114 bp with a 50-UTR region of 79 bp,an open reading frame of 714 bp and a 30-UTR of 302 bp. The deduced protein sequence shows the followingimportant features: leader peptide, a1, a2, and CP/TM/CYT regions. Using long PCR techniques, the genomicsequence was further identified to be 2,093 bp in length,containing four exons and three introns. Three class IIAalleles were identified from four individuals. Two differentalleles observed (at most) in each individual suggest theexistence of a single locus of class IIA gene in the Chineselongsnout catfish genome. This locus is polymorphic andmost of the amino acidic changes occurred in the a1domain containing the peptide-binding region. Lelo-DAAmRNA expression was mainly found in the intestine, gills,spleen, head kidney and liver; minor expression wasdetected in the stomach, skin, heart and muscle. Our resultsyield insight into Chinese longsnout catfish T cell immuneresponses and will aid future work on the genetic status ofthis economically important species.