유기질비료 시비량에 따른 오미자 묘목의 초기 생육특성에 관한 연구

이경아,박태호,박성용,김도현,오문국,김민수,안태진,이상원,김영국,안영섭,박충범,차선우,송범헌 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was carried out to have basic and applied informations to find the optimum nitrogen fertilization level for organic cultivation and to investigate early growth characteristics of Schizandra chinensis Baillon. Different application levels of the organic fertilizer were based on the recommended rate of N fertilizer. Five different levels of the organic fertilizer were applied to S. chinensis and early growth responses such as the length of vine, the diameter of vine, the number of leaves, and the number of branches were investigated. Lengths of main and total vines were shown longer at the level of 1.0 time organic fertilizer than those at others. The vine length at early growth was the highest at the level of 1.0 time organic fertilizer among the treatments. Stem diameter was shown thicker level of 1.0 time organic fertilizer than others on August and September, whereas on October it was the thickest in the conventional cultivation. Number of leaf was increased higher at the level of 1.0 and 2.0 times organic fertilizer than that in the compared to conventional. The number of branches was appeared the lowest at the level of 1.0 time organic fertilizer at early growth, however they were increased through progressing their growth stages. They were not significantly difference among the treatments except for the level of 2.0 times organic fertilizer. Based on the research results, the growth of S. chinensis was commonly higher at the level of 1.0 time organic fertilizer compared to other treatments.

Expression of Cytoplasmic 8-oxo-Gsn and MTH1 Correlates with Pathological Grading in Human Gastric Cancer

Song, Wen-Jie,Jiang, Ping,Cai, Jian-Ping,Zheng, Zhi-Qiang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: Cancers have dysfunctional redox regulation resulting in production of reactive oxygen species (ROS), damaging DNA, RNA and free NTPs, and causing the accumulation of oxidative nucleic acids in cytoplasm. The major types are 8-oxo-7,8-dihydroguanine(8-oxoGsn) in RNA and 8-oxo-7,8-dihydro-2' deoxyguanosine(8-oxodGsn) in Mt-DNA. The MTH1 protein sanitizes oxidized nucleotide pools from NTPs to monophosphates, preventing the occurrence of transversion mutations. This study concerned cytoplasmic 8-oxodGsn/Gsn and MTH1 expression in gastric cancer and para-cancer tissues and elucidated roles of nucleic-acid oxidation and anti-oxidation. Materials and Methods: A polymer HRP detection system was used to detect 8-oxo-Gsn/dGsn and MTH1 expression in 51 gastric cancer and para-cancer tissue samples. Analyses of patient clinical and pathological data were also performed. Results: The expression of MTH1 and the 8-oxo-dGsn/Gsn ratio were significantly higher in cancer tissues than para-cancer tissues (P<0.05). Cytoplasmic 8-oxo-Gsn and MTH1 were both found to positively correlate (P<0.05) with tumor differentiation, while no significant associations were found with gender, age, invasion depth, lymph node metastasis and clinical stage (P>0.05). Conclusions: We found 8-oxo-dGsn/Gsn and MTH1 are both highly expressed in gastric cancer tissues, especially in well differentiated lesions. In addition, oxidated mtDNA is prevalently expressed in gastric cancers, while 8-oxo-Gsn expression in cytoplasmic RNA is a bit lower, but more selectively.

A Silicon Compiler Based on Gate Matrix Method

Wen, Lin Ni,Li, Bo,Song, Jun De 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

This paper presents a new silicon compiler based on Gate Matrix(GM) Method. This system accomplishes the design from logic schematic to gate matrix layout automatically. Our previous work on Gate Matrix Automatic Design System(GMADSII) is introduced in [1]. In this paper, we put the emphasis on the further improvements of GMADSII, which include EDIF compiler, cell library, data converter from logic schematic to transistor level schematic and improved Gate Matrix silicon compiler.

Differentiation of cultivation sources of Ganoderma lucidum by NMR-based metabolomics approach

Wen, He,Kang, Sunmi,Song, Youngmin,Song, Yonghyun,Sung, Sang Hyun,Park, Sunghyouk John Wiley Sons, Ltd. 2010 Phytochemical analysis Vol.21 No.1

<P>Introduction – Ganoderma lucidum is a widely used and high-value medicinal natural product. Correct identification of its cultivation source is important for proper quality assurance, but is so far mostly dependent on subjective morphological examinations.</P><P>Objective – To develop an efficient way of discriminating the cultivation sources of Ganoderma lucidum, particularly those from Korea and China, the two major sources of the mushroom using NMR-based metabolomic differentiation.</P><P>Methodology – Ganoderma lucidum samples were collected from Korea (26 samples) and China (20 samples), and their NMR spectra were obtained. The raw data were processed, and analysed using multivariate statistical analysis.</P><P>Results – Although conventional principal component analysis showed some overlaps, orthogonal projections to latent structure discriminant analysis (OPLS-DA) provided clean distinction between samples from the two countries. Contributing signals were also identified using S-plot, and further verified with independent t-test. Final validation of the model was obtained using prediction test of the unknowns, where the model predicted all of the 14 test samples correctly. Distinction between the cultivation sources within China was also established.</P><P>Conclusion – The easiness and transferability of our NMR-based approach should contribute to addressing an important aspect of quality control process of Ganoderma lucidum. We believe the method can be easily applied to other herbal medical products. Copyright © 2009 John Wiley & Sons, Ltd.</P>

Research Articles : Synchronized Expression of Two Bombyx mori Caspase Family Genes, ice-2 and ice-5 in Cells Induced by Ultraviolet Irradiation

( Wen Bing Wang ),( Ying Sun ),( Li Na Song ),( Yan Wu ),( Hui Ling Wu ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.17 No.1

The caspase family proteins play an important role in programmed cell death (apoptosis). To date, the expression profiles of the caspase family genes in Bombyx mori (Bm) are poorly known. In this study, we examined the expression profiles of two novel Bm caspase family genes (ice-2 and ice-5), the potential change of the mitochondrial membrane and the morphology in Bm cells after stimulation of ultraviolet (UV) irradiation. The results showed the potential change of the mitochondrial membrane occurred at 5 hours after UV irradiation treatment. Analysis of fluorescent quantitative RT-PCR demonstrated that both the ice-2 and ice-5 might be involved in UV induced apoptosis in Bm cells. Notably, after UV irradiating, expression pattern of ice-2 and ice-5 were remarkably different. The ice-2 gene was highly expressed at two time points, 0.5 and 5 hours after UV stimulating, while the expression level of ice-5 only peaked at 5 hours after UV stimulating. It indicated that apoptosis induced by UV irradiation was involved in the mitochondrial pathway and the two isoforms of Bm ice may act but play different role during the apoptosis of Bm cells.

Targeting Orthotopic Glioma in Mice with Genetically Engineered Salmonella typhimurium

Wen, Min,Jung, Shin,Moon, Kyung-Sub,Jiang, Shen Nan,Li, Song-Yuan,Min, Jung-Joon The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.55 No.3

Objective : With the growing interests of bacteria as a targeting vector for cancer treatment, diverse genetically engineered Salmonella has been reported to be capable of targeting primary or metastatic tumor regions after intravenous injection into mouse tumor models. The purpose of this study was to investigate the capability of the genetically engineered Salmonella typhimurium (S. typhimurium) to access the glioma xenograft, which was monitored in mouse brain tumor models using optical bioluminescence imaging technique. Methods : U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. After tumor formation, attenuated S. typhimurium expressing bacterial luciferase (Lux) was injected into the tail vein. Bioluminescence signals from transfected cells or bacteria were monitored using a cooled charge-coupled device camera to identify the tumor location or to trace the bacterial migration. Immunofluorescence staining was also performed in frozen sections of mouse glioma xenograft. Results : The injected S. typhimurium exclusively localized in the glioma xenograft region of U87-MG-bearing mouse. Immunofluorescence staining also demonstrated the accumulation of S. typhimurium in the brain tumors. Conclusion : The present study demonstrated that S. typhimurium can target glioma xenograft, and may provide a potentially therapeutic probe for glioma.

In situ Synthesis of p–n LaFeO3/ZnIn2S4 Heterojunctions for Enhanced Photocatalytic Activity

Wen Gao,Chengjia Zhang,Sainan Cui,Qian Liang,SONG XU,ZHONGYU LI 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.8

In this study, LaFeO3/ZnIn2S4 composites were synthesized via in situ synthesis. The composition, structure and optical absorption properties of LaFeO3/ZnIn2S4 were characterized by X-ray diffraction (XRD), ultraviolet-visible diffuse reflectance spectroscopy, fluorescence spectroscopy (PL), Fourier Transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). The photocatalytic activity of the LaFeO3/ZnIn2S4 photocatalyst was determined based on the degradation of methyl orange (MO). LaFeO3/ZnIn2S4 composites showed much better photocatalytic performance compared with pure LaFeO3 and ZnIn2S4. The enhanced photocatalytic performance was attributed to intimately contacted interfaces and charge transfer channels which can effectively transfer and separate the photogenerated charge carriers.

The molecular mechanisms and factors affecting the feeding habits of silkworm (Lepidoptera: Bombyxidae)

Song Wen-Ting,Zhu Fei-Fei,Chen Keping 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4

Food is critical to the survival and development of insects. Bombyx mori (Lepidoptera: Bombycidae), a Lepi doptera model insect with economic significance, is a well-known oligophagous insect that mainly feeds on mulberry leaves. The feeding characteristics of this particular species provide an excellent model for studying the food selection of insect host plants. In recent years, there has been an increasing number of studies on the factors affecting the feeding of silkworms, especially with the development of molecular technology. Many mysteries have been gradually solved. This paper summarizes and discusses the factors that affect the feeding of silkworms, with a focus on the molecular mechanisms of silkworm feeding. It is hoped that this work will be helpful for further research on artificial diets for silkworms and disease control in lepidopteran insects.

Urinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkers

Wen, He,Lee, Tack,You, Sungyong,Park, Soo-Hwan,Song, Hosook,Eilber, Karyn S.,Anger, Jennifer T.,Freeman, Michael R.,Park, Sunghyouk,Kim, Jayoung American Chemical Society 2015 JOURNAL OF PROTEOME RESEARCH Vol.14 No.1

<P/><P>Interstitial cystitis/painful bladder syndrome (IC) is a chronic syndrome of unknown etiology that presents with bladder pain, urinary frequency, and urgency. The lack of specific biomarkers and a poor understanding of underlying molecular mechanisms present challenges for disease diagnosis and therapy. The goals of this study were to identify noninvasive biomarker candidates for IC from urine specimens and to potentially gain new insight into disease mechanisms using a nuclear magnetic resonance (NMR)-based global metabolomics analysis of urine from female IC patients and controls. Principal component analysis (PCA) suggested that the urinary metabolome of IC and controls was clearly different, with 140 NMR peaks significantly altered in IC patients (FDR < 0.05) compared to that in controls. On the basis of strong correlation scores, fifteen metabolite peaks were nominated as the strongest signature of IC. Among those signals that were higher in the IC group, three peaks were annotated as tyramine, the pain-related neuromodulator. Two peaks were annotated as 2-oxoglutarate. Levels of tyramine and 2-oxoglutarate were significantly elevated in urine specimens of IC subjects. An independent analysis using mass spectrometry also showed significantly increased levels of tyramine and 2-oxoglutarate in IC patients compared to controls. Functional studies showed that 2-oxoglutarate, but not tyramine, retarded growth of normal bladder epithelial cells. These preliminary findings suggest that analysis of urine metabolites has promise in biomarker development in the context of IC.</P>

A collaborative client participant fusion system for realistic remote conferences

Song, W.,Wen, M.,Xi, Y.,Chu, P. M.,Vu, H.,Kayumiy, S. J.,Cho, K. Springer Science + Business Media 2016 The Journal of supercomputing Vol.72 No.7

<P>Remote conferencing systems provide a shared environment where people in different locations can communicate and collaborate in real time. Currently, remote video conferencing systems present separate video images of the individual participants. To achieve a more realistic conference experience, we enhance video conferencing by integrating the remote images into a shared virtual environment. This paper proposes a collaborative client participant fusion system using a real-time foreground segmentation method. In each client system, the foreground pixels are extracted from the participant images using a feedback background modeling method. Because the segmentation results often contain noise and holes caused by adverse environmental lighting conditions and substandard camera resolution, a Markov Random Field model is applied in the morphological operations of dilation and erosion. This foreground segmentation refining process is implemented using graphics processing unit programming, to facilitate real-time image processing. Subsequently, segmented foreground pixels are transmitted to a server, which fuses the remote images of the participants into a shared virtual environment. The fused conference scene is represented by a realistic holographic projection.</P>