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- 저자 : Wenfeng Ke (검색결과 2 건)
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Hao Wang,Liang Wang,Wenfeng Shen,Ke Cao,Lei Sun,Pengju Wang,Liyun Cui 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.2
Recycled concrete powder (RCP) recycling can reduce the consumption of natural resources, which has been highlighted as an ecofriendly and sustainable approach. The alkali-activated slag (AAS) offers notable prospects for replacing conventional Portland cement binders. In this study, we partially replaced granulated blast furnace slag (GBFS) with RCP to prepare the AAS mortars. We evaluated the effect of RCP replacement levels and fineness levels on compressive strength, early hydration process, and pore structure pertaining to AAS mortars with RCP (RCP-AAS mortars). According to the experimental results, using 10 – 30% RCP inside AAS mortars enhanced the compressive strength at 1 day, and the improvement was obvious with increasing RCP fineness. The RCP delayed the early-age hydration of the GBFS, decreased the content of hydration products and increased the total porosity. Therefore, it exhibited a negative effect on 28-day compressive strength. These drawbacks of RCP, however, could be modified by increasing its fineness.
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Rapid Screen of IL-5/IL-5Rα Blocking Antibodies in the HEK293-IL-5Rα-CSF2RB Transfected Cell Line
Shijie Li,Fei Han,Chang Liu,Weiyan Dai,Wenfeng Ke,Yongqi Chen,Eric Fordjour,Yankun Yang,Zhonghu Bai 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.4
Interleukin-5 (IL-5) binding to interleukin-5 receptor subunit alpha (IL-5Rα) increases the number of eosinophils and enhances eosinophil activity. This leads to eosinophil tissue infiltration and damage to the lungs, ultimately resulting in exacerbation of asthma. Antibodies that block IL-5 binding to IL-5Rα are thought to play an important role in advanced asthma. Currently, key methods used to screen for targeted drugs are Surface Plasmon Resonance which is costly and anti-proliferation assays which are tedious and have a low signal-to-noise ratio. Here we describe a Fluorescence Activated Cell Sorting (FACS) assay, based on human embryonic kidney (HEK)- 293 cells with stable expression of IL-5Rα and the cytokine receptor common subunit beta (CSF2RB). Cells co-expressing IL-5Rα and CSF2RB had a 16% increase in the ability to bind IL-5 compared to cells expressing only IL-5Rα. The optimal concentration of IL-5 for the FACS assay was 0.1 μg/mL. The established FACS was used to screen anti IL-5 nanobodies and hybridoma supernatants for candidate antibodies that block the IL-5/IL-5α interaction. When compared to anti-proliferation assays, this method saved up to 90% of the assay time, offering the advantage of rapidity and accuracy in vitro. The assay described here provides a novel approach for rapid screening of IL-5/IL- 5Rα blocking antibodies in vitro to accelerate the development of drugs for asthma.
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