IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis

Wenfang Xu,Yanan Wang,Changzhong Jin,Weiyang Zhang,Jiangnan Chen,Xuefang Chen,Junli Gao,Junshun Gao,Hong Wang 대한면역학회 2023 Immune Network Vol.23 No.6

Autoimmune hepatitis (AIH) affects all age group and occurs mainly in women. Pyroptosis is a novel programmed cell death featured with cell bursting and release of proinflammatory cytokines. A deeper understanding of AIH pathogenesis will contribute to novel therapy for AIH patients. Here, we aimed to investigate the role of IL-17 in immune-mediated liver injury. The levels of cytokines were measured by ELISA, and mRNA levels of STAT3 and IFN gammainducible protein 16 (IFI16) were detected by PCR. Expressions of STAT3, IFI16, gasdermin D and cleaved caspase-1 were measured by western-blotting. Immunohistochemical staining and transmission electron microscopy were applied to evaluate liver histopathological changes of the treated mice. Our results showed that the levels of IFI16 was increased in hepatocytes treated with IL-17 protein, and further elevated after STAT3-overexpressed (STAT3-OE) lentivirus treatment. The levels of IFI16 were reduced in hepatocytes treated with IL-17 neutralizing Ab (nAb), but were significantly increased after STAT3-OE treatment. Pyroptosis was observed in hepatocytes treated with IL-17 protein, and further cell damage was observed after STAT3-OE lentivirus treatment. Liver damage was alleviated in mice treated with IL-17 nAb, however sever damage was experienced after STAT3-OE lentivirus treatment. A binding interaction between IFI16 and STAT3 was detected in IL-17 treated hepatocytes. Glutathione transaminase activity was enhanced in concanavalin A-induced AIH mice compared to the control group (p<0.01). IL-17 plays an important role in activating STAT3 and up-regulating IFI16, which may promote the pyroptosis in AIH-related liver injury through STAT3-IFI16 axis.

Long-core experimental study of different displacement modes on fractured-vuggy carbonate reservoirs

Wenfang Li,Jiang Chen,Xiqun Tan,Ping Liu,Yun Li,Limin Wang 한국자원공학회 2018 Geosystem engineering Vol.21 No.2

It is difficult to utilize real fractured–vuggy carbonate cores to conduct long-core displacement experiments due to the special structures of fractured and vuggy. Aiming to not affect the porosity and permeability of the original core structure, a special filling method on the sunken space of the core surface was created to meet the requirements of long-core experiments. Long-core experiments of depletion drive, water flooding, gas drive, and water alternating gas (WAG) drive were performed to simulate the actual conditions of one-dimension oil reservoir and systematically study the influence of different development approaches on the recovery of vuggy carbonate reservoirs. Results show that the optimal conversion pressure of water flooding is between 80% bubble point and bubble point pressure. In the high-pressure and high water-cut stage, the development effect of gas–water alternative drive becomes significant, it can effectively reduce water cut and improve oil recovery. The most suitable and effective displacement mode is adopted depletion drive first and converts water flooding when the pressure decreases between 80% bubble point and bubble point pressure. When the water cut approximately reaches 80%, then WAG flooding is performed. The experiments provide data and technical support for the rational development of vuggy carbonate reservoirs.

NONLINEAR OPTICAL PROPERTIES OF THE WURTZITE InGaN/AlGaN PARABOLIC QUANTUM DOT

TAIRONG CHEN,Wenfang Xie,Shijun Liang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.2

The linear and nonlinear optical properties in a wurtzite InxGa1-xN/AlyGa1-yN strained spherical quantum dot (QD) subjected to a uniform external electric field with a direction opposite to that of the built-in electric (BEF) within the framework of effective-mass approximation have been investigated. The results show that the BEF has great effects on the linear and nonlinear optical absorption coefficients (ACs) and refractive index (RI) changes. Moreover, the dependence of the linear and nonlinear optical properties on the In composition, on the Al composition and on the inner sphere radius has been studied in detail. It is indicated that the ACs and RI changes of the Wurtzite spherical QD have strongly affected by these factors. The results may make a significant contribution to designing some important photodetectors and photonic crystal devices.

Removal of UO2 2+ from aqueous solution using halloysite nanotube-Fe3O4 composite

Yuantao Chen,Wenfang He,Wei Zhang,Chunlian Hu,Jian Wang,Pingping Wang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.1

Halloysite nanotubes (HNTs) were modified with Fe3O4 to form novel magnetic HNTs-Fe3O4 composites, and the composites were characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FT-IR) and vibrating sample magnetometer (VSM). The as-obtained results indicated that Fe3O4 nanoparticles were successfully installed on the surface of HNTs. The adsorption of UO2 2+ on HNTs-Fe3O4 was investigated as a function of solid content, contact time, pH, ionic strength and temperature by batch experiments. The consequences revealed that the adsorption of UO2 2+ onto HNTs-Fe3O4 was strongly dependent on pH and ionic strength. Equilibrium data fitted well with the Langmuir isotherm. The experimental results demonstrated that the adsorbents with HNTs-Fe3O4 had the largest adsorption capacity of 88.32mg/g for UO2 2+.

64kb/s Multi-function Terminal Manufactured for ISDN

Donghan Jin,Hongtao Wang,Wenfang Chen 대한전자공학회 1991 ICEIC:International Conference on Electronics, Inf Vol.1991 No.8

Rapid adsorption of cobalt (II) by 3-aminopropyltriethoxysilane modified halloysite nanotubes

Xin Wang,Yuantao Chen,Wei Zhang,Wenfang He,Jian Wang,Biqing Chen 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.12

The adsorption of cobalt (II) by 3-aminopropyltriethoxysilane (APTS) modified halloysite nanotubes (HNTs) was studied. The modified halloysite was characterized by Fourier transform infrared spectroscopy (FT-IR), XRay diffraction (XRD) and thermogravimetry (TG). In addition, a batch method was used to study the adsorption behavior and the mechanism of removal Co (II) by modified HNTs; the effective factors influencing the adsorption process including adsorbent concentration, contact time, initial pH, temperature and the initial concentration of Co (II) have been investigated. The results showed that the APTS was successfully grafted on the HNTs surface. The modified HNTs exhibited rapid adsorption speed, which reached the adsorption equilibrium within 30 min. The adsorption capacity of the adsorbent increased significantly with the increase of pH. The adsorption data of Co (II) on the modified HNTs are well consistent with the Langmuir model and the pseudo-second-order kinetic model.

Overview of the Application of Flow Microreactors in the Synthesis of Silver Nanomaterials

Xiaoai He,Aijuan Lu,Jin Cheng,Junfang Chen,Qianhui Song,Wenfang Liu,Chuanpin Chen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.11

The unique electrical, optical and biological properties of silver nanomaterials have attracted significant attention of many researchers. Since the size and shape of silver nanomaterials have significant effects on the properties of silver nanomaterials, extensive research has focused on synthesis and characterization of silver nanomaterials. However, almost all of the syntheses of silver nanomaterials were carried out in traditional batch reactors, which typically suffer from inhomogeneous mixing and corresponding spatial variations under reaction conditions, ultimately leading to poor quality of the final nanomaterials. Recently, the emerging microfluidic technology not only furnishes novel strategies for the synthesis of silver nanomaterials but also brings great opportunities and impetus to improve the quality and yield of silver nanomaterials due to enhanced mass and heat transfer. The current paper reviews recent achievements in the synthesis of silver nanomaterials in flow microreactors. Various strategies adopted for the synthesis of silver nanomaterials in microreactors are presented and compared, including synthesis in single-phase and multi-phase flow microreactors. In addition, the factors that affect the size and size distribution of silver nanomaterials in flow microreactors synthesis are also discussed briefly.