http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Usama K. Moawad (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Korni, Fatma M. M.,Moawad, Usama K.,Mohammed, Asmaa N.,Edrees, Asmaa The Korean Society of Fish Pathology 2022 한국어병학회지 Vol.35 No.1
Aquaculture development is based on the ideas of increasing production while reducing economic losses. Bacterial diseases are the leading source of fish cases. Citrobacter freundii has been linked to septicemia and mortality all over the world. In the current study, the cause of mortality in O. niloticus was C. freundii MW279218. External hemorrhages were seen on the affected fish, as well as paleness in the liver and kidney congestion. C. freundii MW279218 had a median lethal dosage of 1.5×10<sup>5</sup> CFU/mL. Zinc oxide and zinc oxide nanoparticles (ZnO-NPs) were tested for their biocidal effectiveness against C. freundii MW279218. The lethal effect of ZnO-NPs for C. freundii MW279218 was 100% when compared to zinc oxide compound, and the inhibition zone width was 2.31.1mm at the highest tested concentrations (70 mg/L) compared to the lowest (35 and 45 mg/L, respectively). Fish were fed three different diets for 28 days: diet 1 (no additives), diet 2 (100 mg of ZnO-NPs/kg of feed), and diet 3 (200 mg of ZnO-NPs/kg of feed). Organs were also collected for histopathology 96 hours after injection (P<0.05). In the groups given 200 mg of ZnO-NPs, there was 10% mortality and 80% RPS. The group fed 100 mg of ZnO-NPs/kg, on the other hand, had 20% mortality and 60% RPS, compared to 50% mortality in the control positive group. Histopathological examinations demonstrated significant alterations in the control positive group and mild lesions in the hepatopancreas of the groups administered 100 mg ZnO-NPs/kg of feed. The groups fed 200 mg of ZnO-NPs/kg diet, on the other hand, showed no histological alterations. ZnO-NPs were found to be effective in the up regulation of both IL-10 and complement 5 immune-related genes.
-
Fatma M. M. Korni,Asmaa N. Mohammed,Usama K. Moawad,Nour El-Houda y. Hassan,Ahmed Farghali,Rehab K. Mahmoud 한국어병학회 2023 한국어병학회지 Vol.36 No.1
Nanotechnology seeks to improve material effectiveness to have a greater impact on medicine. The current study was performed to determine how effective dietary propolis (PR) and propolis nanoparticles (PR-NPs) were at improving immune and anti-oxidant status, lowering cortisol levels as stress indicators, and preventing Pseudomonas aeruginosa infection in O. niloticus. Histopathology was carried out. Fish (N = 516, 25 ± 2g) were fed basal diets supplemented with 1 gm PR/kg, 2 gm PR/kg, 1 gm PR-NPs/kg, 2 gm PR-NPs/kg feed and control diet for 28 days. When compared to the control, interlukin-1, IgM, complement 5, and levels of lysozyme were dramatically higher in PR and PR-NPs fed-groups. Antioxidant enzymes and glutathione levels were higher in fish fed PR and PR-NPs, but malondialdehyde levels were lower. Cortisol levels decreased in feeding groups compared to the control. When compared to O. niloticus group fed 1 gm PR/kg and the positive control, propolis nanoparticles successfully prevented P. aeruginosa infection and the mortality rate was zero. When compared to the negative control, the positive control group's histopathological findings revealed severe histopathological changes. In low and high groups fed PR-NPS, normal structures were observed as well as high concentrations of PR after being injected with P. aeruginosa. The group that was fed low concentrations of PR after being injected with P. aeruginosa showed only minor histopathological changes. Conclusion: Dietary supplementation of PR or PR-NPs may have a beneficial effect on aquaculture, with PR-NPs having a superior effect.
-
Fatma M. M. Korni,Fatma I. Abo El-Ela,Usama K. Moawad 한국어병학회 2023 한국어병학회지 Vol.36 No.1
The current study aimed to identify the primary cause of mortality in a commercial Oreochromis niloticus (O. niloticus) farm. Furthermore, the efficacy of poly-β-hydroxybutyrate (PHB) as a feed additive to prevent mortality was investigated after in-vitro testing. Also, a histopathological examination was carried out. The samples of naturally diseased O. niloticus showed swellings and hemorrhages on the body surface. Moreover, the post-mortem examination revealed black fluids with an awful odor, a congested liver, and intestinal gasses. Concerning diagnosis of the main cause of mortality in diseased O. niloticus, , there were eight isolates might be Edwardsiella tarda based on the biochemical identification results. Also, the identification was confirmed by using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The resulting spectra of two previously identified E. tarda strains were then compared to those found in the Bruker database and the two strains were identified as E. tarda at the species level. For detection of pathogenicity of identified strains, the virulence E. tarda hemolysin (ETHA) gene were detected at band 1078 bp in the eight identified strains. Regarding the in-vitro antimicrobial activity of PHB against E. tarda, the antibacterial activity of blood and tissues had been calculated using the Agar and well diffusion procedures Prior to and after the challenge,. Lower bacterial counts and a larger inhibition zone were signs of the tested materials' concentration-dependent antibacterial activity. For in-vivo evaluation of PHB in dietary-fed O. niloticus, PHB was effective in preventing Edwardsiellosis, with the lowest mortality rates in the group fed 10 g PHB/Kg feed after being injected with E. tarda. In comparison, the group that received a 5 g PHB/Kg feed after receiving an injection of E. tarda saw 16.6% mortalities and a 66.7 percent relative survival rate. The control positive group had 50% mortality. The results of the biochemical testes showed that O. niloticus's kidney and liver functions were unaffected by dietary PHB supplementation. Protein, albumin, globulin, urea, creatinine, aspartate transaminase levels were identical to those of the control negative group. According to the current study, the groups fed high and low PHB concentrations had considerably higher levels of immunoglobulin M, complement 5, and interleukin-1 than the control group. The number of total viable bacteria was lower in the gut of PHB-fed groups than in control. Compared to mild histological alterations in those fed with a lower dosage and severe histopathological abnormalities in the control group, the greater dose of PHB effectively prevented Edwardsiellosis in O. niloticus with decreased mortality and no histopathological changes.
ScienceON
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
