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        Storability, post-storage conversion and genetic stability assessment of alginate-encapsulated shoot tips of monopodial orchid hybrid Aranda Wan Chark Kuan 'Blue' ${\times}$ Vanda coerulea Grifft. ex. Lindl

        Gantait, Saikat,Sinniah, Uma Rani 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

        An efficient short-term storage system of synthetic seeds, produced using in vitro shoot tips of the monopodial orchid hybrid Aranda Wan Chark Kuan 'Blue' 9 Vanda coerulea Grifft. ex. Lindl. (AV), was developed. In vitro shoot tips (3-4 mm) were successfully encapsulated, resulting in uniform spherical beads (capsules), using 3 % sodium alginate with 75 mM $CaCl_2{\cdot}2H_2O$. Maximum (~100 %) conversion (int plantlets with shoot and root) of capsules (or synthetic seeds) was achieved on quarter-strength Murashige and Skoog regrowth medium, while full-strength MS medium was required for effective conversion of non-encapsulated shoot tips. The capsules showed distinct difference in their response to temperature during storage. The conversion efficiency declined upon storage duration at both 4 and $25^{\circ}C$, with those stored at $25^{\circ}C$ being more tolerant storage. Capsules stored at $4^{\circ}C$ had rapid deterioration an faced complete death within 160 days while those stored for 200 days at $25^{\circ}C$ showed relatively high conversio (71.6 %). An inter-simple sequence repeats fingerprinting approach, employed on indiscriminately chosen plantlets from converted capsules (following 4 and $25^{\circ}C$ of storage) ensured the post-storage genetic stability.

      • KCI등재

        Storability, post-storage conversion and genetic stability assessment of alginate-encapsulated shoot tips of monopodial orchid hybrid Aranda Wan Chark Kuan ‘Blue’ 3 Vanda coerulea Grifft. ex. Lindl.

        Saikat Gantait,Uma Rani Sinniah 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

        An efficient short-term storage system of syntheticseeds, produced using in vitro shoot tips of themonopodial orchid hybrid Aranda Wan Chark Kuan‘Blue’ 9 Vanda coerulea Grifft. ex. Lindl. (AV), wasdeveloped. In vitro shoot tips (3–4 mm) were successfullyencapsulated, resulting in uniform spherical beads (capsules),using 3 % sodium alginate with 75 mMCaCl22H2O. Maximum (*100 %) conversion (intoplantlets with shoot and root) of capsules (or syntheticseeds) was achieved on quarter-strength Murashige andSkoog regrowth medium, while full-strength MS mediumwas required for effective conversion of non-encapsulatedshoot tips. The capsules showed distinct difference in theirresponse to temperature during storage. The conversionefficiency declined upon storage duration at both 4 and25 C, with those stored at 25 C being more tolerant tostorage. Capsules stored at 4 C had rapid deterioration andfaced complete death within 160 days while those storedfor 200 days at 25 C showed relatively high conversion(71.6 %). An inter-simple sequence repeats fingerprintingapproach, employed on indiscriminately chosen plantletsfrom converted capsules (following 4 and 25 C of storage),ensured the post-storage genetic stability.

      • KCI등재
      • KCI등재

        Yield Improvement by Two Cycles of Mass Selection in Two Sweet Corn Populations

        Nigussie Mandefro,Saleh Ghizan,Wahab Zakaria,Sinniah Uma Rani The Korean Society of Crop Science 2005 Korean journal of crop science Vol.50 No.2

        Mass selection (MS) is an efficient selection method to directly improve highly heritable traits. In the present study, two cycles of MS for ear length were conducted on two sweet corn populations, BC2-l0 and $BC1-10{\times}Syn-II$ after introgression of exotic germplasm. The improved populations generated from these selections were evaluated in comparison with the base populations at two locations, to determine the genetic gains and performance of the improved populations. The two base populations showed varied average realized responses to MS. In BC2-l0 derived population, the realized responses were $9.1\%$ in BC2-l0 C1 and $1.2\%$ in BC2-l0 C2, whereas in$BC1-10{\times}Syn-II$ derived population, the realized responses were$ 5.6\%$ in $BC1-10{\times}Syn-II$ C1 and $2.9\%$ in $BC1-10{\times}Syn-II$ C2. All the improved populations showed longer ears than their respective base populations and the check varieties. Ear length, which was used as the selection criterion in this study, showed high broad-sense heritability in the BC2-l0 and $BC1-10{\times}Syn-II$ derived populations, while fresh ear yield revealed low heritability, indicating that selection for ear length in these populations would be more effective than direct selection for yield. Results of this study indicate that MS conducted on BC2­10 and $BC1-10{\times}Syn-II$ had significantly increased ear length and fresh ear yield in both populations. The improved populations obtained would serve as better germplasm sources and further selection in these populations could offer better responses.

      • KCI등재

        Ethnomedicinal uses, phytochemistry, pharmacological properties and toxicology of Curcuma caesia Roxb.: a review

        Zainol Haida,Jaafar Juju Nakasha,Uma Rani Sinniah,Mansor Hakiman 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.4

        Curcuma caesia or commonly known as black turmeric is belongs to family Zingiberaceae. The rhizome which is the most important part of this species is widely used as a folk medicine for the treatment of asthma, fever, cancer, wounds, allergies, toothache, leprosy, bronchitis, epilepsy, hemorrhoids, leukoderma and rheumatoid arthritis. C. caesia is one of the important species of Curcuma which possess various bioactive compounds that responsible for numerous pharmacological activities. The rhizome which is the most prominent feature of the plant is rich with essential oil. In addition, the leaves of this species also consist of essential oil with various bioactive compounds. This review article is aimed to discuss in-depth on botany, ethnomedicinal uses, geographical distribution, propagation, phytochemical studies, pharmacological activities and toxicity of C. caesia. The phytochemical studies revealed that a total of 17 functional groups were detected from rhizome extract of C. caesia. The pharmacological studies conducted demonstrated that C. caesia extract exhibited anti-acne, analgesic, anthelmintic, anti-asthmatic, anti-inflammatory, anti-bacterial, anti-fungal, anti-diabetic, antiproliferative, anticancer, antiulcer, hepatotoxicity, nephrotoxicity, neurotoxicity and cytotoxicity effects. Furthermore, toxicity studies revealed that C. caesia extract is safe for consumption and does not cause toxicity.

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