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- 저자 : Syamala, D. (검색결과 4 건)
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Syamala, D.,Devi, Prathibha The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.2
Development of efficient plant regeneration and genetic transformation protocols (using the Particle Inflow micro-projectile Gun and the shoot-tips as target tissue) of Sorghum bicolor (L.) Moench in terms of expression of the reporter gene, $\beta$-glucuronidase(uidA) is reported here. Two Indian cultivars of sorghum were used in the study, viz. M-35-1 and CSV-15. Plant regeneration was achieved from one-week-old seedling shoot-tip explants via multiple-shoot-clumps and also somatic embryos. The multiple-shoot-clumps were produced on MS medium containing BA (0.5, 1.0 or 2.0 mg/$L^{-1}$), with biweekly subculture. Somatic embryos were directly produced on the enlarged dome shaped expansive structures that developed from shoot-tip explants (without any callus formation) when cultured on MS medium supplemented both with BA (0.5, 1.0 or 2.0 mg/$L^{-1}$) and 2,4-D (0.5 mg/$L^{-1}$). Whereas each multiple-shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds, the somatic embryos were capable of 90% germination, plant conversion and regeneration. The regenerated shoots could be efficiently rooted on MS medium containing 1.0mg/$L^{-1}$ IBA and successfully transplanted to the glasshouse and grown to maturity with a survival rate of 92%. The plant regeneration efficiency of both the genotypes were similar. After the micro-projectile bombardment, expression of uidA gene was determined by scoring blue transformed cell sectors in the bombarded tissue by an in situ enzyme assay. The optimal conditions comprising a helium pressure of 2200 K Pa, the target distance of 11 cm with helium inlet fully opened and the use of osmoticum have been defined to aid our future strategies of genetic engineering in sorghum with genes for tolerance to biotic and abiotic stresses.
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Agrobacterium tumefaciens Mediated Genetic Transformation of Pigeonpea [Cajanus cajan (L.) Millsp.]
Kumar, S.Manoj,Syamala, D.,Sharma, Kiran K.,Devi, Prathibha The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.2
Optimal protocol for efficient genetic transformation has been defined to aid future strategies of genetic engineering in pigeon pea with agronomically important genes. Transgenic pigeonpea plants were successfully produced through Agrobacterium tumefaciens-mediated genetic transformation method using cotyledonary node explants by employing defined culture media. The explants were co-cultivated with A. tumefaciens strain C-58 harboring the binary plasmid, pCAMBIA-1301 [con-ferring $\beta$-glucuronidase(GUS) activity and resistance to hygromycin] and cultured on selection medium (regeneration medium supplemented with hygromycin) to select putatively transformed shoots. The shoots were then rooted on root induction medium and transferred to pots containing sand and soil mixture in the ratio of 1:1. About 22 putative TO transgenic plants have been produced. Stable expression and integration of the transgenes in the putative transgenics were confirmed by GUS assay, PCR and Southern blot hybridization with a transformation efficiency of over 45%. Stable integration and expression of the marker gene has been confirmed in the TO and T1 transgenics through PCR, and Southern hybridization.
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Machine learning-based design automation of CMOS analog circuits using SCA-mGWO algorithm
Vijaya Babu E,Syamala Y 한국전자통신연구원 2022 ETRI Journal Vol.44 No.5
Analog circuit design is comparatively more complex than its digital counterpart due to its nonlinearity and low level of abstraction. This study proposes a novel low-level hybrid of the sine-cosine algorithm (SCA) and modified greywolf optimization (mGWO) algorithm for machine learning-based design automation of CMOS analog circuits using an all-CMOS voltage reference circuit in 40-nm standard process. The optimization algorithm’s efficiency is further tested using classical functions, showing that it outperforms other competing algorithms. The objective of the optimization is to minimize the variation and power usage, while satisfying all the design limitations. Through the interchange of scripts for information exchange between two environments, the SCA-mGWO algorithm is implemented and simultaneously simulated. The results show the robustness of analog circuit design generated using the SCAmGWO algorithm, over various corners, resulting in a percentage variation of 0.85%. Monte Carlo analysis is also performed on the presented analog circuit for output voltage and percentage variation resulting in significantly low mean and standard deviation.
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Protective Effect of Pterocarpus santalinus onGalactosamine Induced Liver Damage
S. P. Dhanabal,G. Syamala,K. Elango,B. Suresh 한국생약학회 2006 Natural Product Sciences Vol.12 No.1
Pterocarpus santalinus Linn on acute hepatotoxicity induced in Wistar albino rats by a single dose ofGalactosamine (400 mg/kg). Suspensions of methanolic extract of heartwood of P. santalinus (200 and 400 mg/kg)in 0.3% Carboxy Methyl Cellulose (CMC) were administered p.o. to experimental animals and hepatoprotectiveactivity was monitored by estimating aspartate amino transferase (ASAT, GOT), alanine amino transferase(ALAT, GPT), alkaline phosphatase (ALP), total bilirubin (TB), lactate dehydrogenase (LDH), total cholesterolelevation of serum transaminases and alterations of biochemical parameters induced by hepatotoxin, andalleviated the degree of liver damage. The results were supported by histopathological studies of liver samplesshowing regeneration of hepatocytes in treated animals. Silymarin (25 mg/kg), a known hepatoprotective drug wasused for comparison. Based on the results obtained, it can be concluded that P. santalinus exerts hepatoprotectiveactivity and may serve as a useful adjuvant in several clinical conditions associated with liver damage.Keywords Pterocarpus santalinus
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