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Sungsic Bang,Jeehye Seo,Teaho Kim,Dongho Choi,Zhuqing Wu,Jongmin Lee,Jinho Jo 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.4
Acquiring real-time microscopic images directly from laboratory animals is technically difficult. Fluorescencemediated observation of blood vessels and developing tumors is particularly challenging. We developed a titanium and polyethylene tissue window to observe under the skin and evaluated the feasibility of this approach in a rat model. The prerequisites of the tissue window were absence of foreign body reaction, high physical strength, and reduced invasiveness. Foreign body reaction was monitored for 1 week following installation on the back of each animal. Following the injection of fluorescent material, subcutaneous venous flow, tumor neovascularization flow, and cerebral surface vascular flow could be imaged using the tissue window.