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Interfacing biology with nanoparticles
Saikat Mandal,Sumant Phadtare,Murali Sastry 한국물리학회 2005 Current Applied Physics Vol.5 No.2
New approaches are constantly being developed for both the synthesis of inorganic nanomaterials and their surface modicationfor sensing and electronic applications. Insofar as surface modication of gold nanoparticles is concerned, thiol chemistry is themost popular approach to bind ligands to their surface. We have been pursuing the possibility of using amine functionality to bindligands to the surface of gold nanoparticles and have found that amine binding is as strong as thiol binding. The advantages of usingamine chemistry for surface modication of nanogold are many, the possibility of complexing a large variety of biomolecules such asamino acids and proteins being one of the most important. In this article, we review the work from this laboratory on the stabili-zation of gold nanoparticles using amino acids as well as using amino acids as reducing agents to obtain stable aqueous solutions ofgold nanoparticles of variable size. We also discuss the possibility of forming bioconjugates of enzymes with gold nanoparticles dec-orating the surface of polymer microspheres and their application as reusable biocatalysts. There is much to be gained by marryingnanomaterials with biology with considerable spin-os likely in both nanotechnology and biotechnology.. 2004 Elsevier B.V. All rights reserved.PACS:81.07.Bc; 81.16.Be; 81.16.Dn; 82.35.Np; 87.14.Ee
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