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Sulaiman Al-Zuhair,Khalda Ahmed,Ashir Abdulrazak,Muftah H. El-Naas 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.2
The sequential addition of the enzymes, laccase for lignin degrading, followed by xylanase for hemicelluloses hydrolysis, then cellulase for cellulose hydrolysis, was compared to the synergistic action of using the three enzymes together. It was shown that the reducing sugars yield increased from 5.6%using cellulase only to 45.6% by pretreatment with laccase and xylanase, prior to the enzymatic hydrolysis. A higher conversion of 60% was achieved by using the three enzymes together for the same incubation period. The proposed synergistic enzymes approach is a simpler and less energy intensive alternative compared to the conventional lignocelluloses pretreatment techniques.
Batch degradation of phenol in a spouted bed bioreactor system
Muftah H. El-Naas,Souzan Makhlouf,Sulaiman Al-Zuhair 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.2
Bacterial biotreatment has gained a lot of attention in recent years as an alternative method for the removal of phenol from wastewater. Under aerobic conditions, Pseudomonas putida utilize phenol as a source of carbon and energy. In this work, P. putida was immobilized in Polyvinyl Alcohol (PVA) gel particles and used in a spouted bed bioreactor to remove phenol from wastewater. The effects of initial phenol concentration and air flow rate on the rate of aerobic phenol removal were investigated. The experimental results were used to develop a dynamic model to describe the utilization of phenol for the bacterial growth in spouted bed bioreactor.