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      저자 : Sirrar Mesadhi (검색결과 1 건)
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        Chitosan-based bioglass composite for bone tissue healing : Oxidative stress status and antiosteoporotic performance in a ovariectomized rat model

        Samira Jebahi,Hassane Oudadesse,Gada Ben Saleh,Mongi Saoudi,Sirrar Mesadhi,Tarek Rebai,Hassib Keskese,Abdelfattah Elfeki,Hafed El Feki 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.9

        Tissue engineering has opened up a new therapeutic avenue promising a revolution in regenerative medicine. Considerable attention has been given to chitosan composite materials and their applications in the field of the bonegraft substitutes. We evaluated the antioxidative properties of chitosan-doped bioactive glass (BG-CH) with 17 wt%chitosan, and their applications in the guided bone regeneration. BG-CH was produced by a freeze-drying process andimplanted in the femoral condyles of ovariectomized rats. Grafted bone tissues were carefully removed to evaluate theoxidative stress analysis, histomorphometric profile and mineral bone distribution by using inductively coupled plasmaoptical emission spectrometry (ICP-OES). A significant decrease of thiobarbituric acid-reactive substances (TBARs)was observed after BG-CH implantation. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase(GPx) activities significantly increased in ovariectomized group implanted with chitosan-doped bioactive glass (OVX-BG-CH) as compared to ovariectomized group implanted with bioactive glass (OVX-BG). The histomorphometricanalysis showed that bone/tissue volume (BV/TV), osteoblast number (N.Ob) and osteoblast surface/bone surface (Ob.S/BS) were significantly higher in OVX-BG-CH group than in OVX-BG group. On the other hand, a rise in Ca and Pion concentrations in the implanted microenvironment was shown to lead to the formation/deposition of Ca-P phases. Trace elements such as Sr and Fe were detected in the newly formed bone and involved in bone healing. These resultssuggested that BG-CH composites could become clinically useful as a therapeutic and implantable material.

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