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        Study of fertility restoration and genetic diversity of drought-tolerant breeding lines for hybrid rice (Oryza sativa L.) development

        Singh Vijayata,Priyadarshi Rahul,Singh Akhilesh Kumar,Jain Abhinav 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.1

        The identifcation of fertility restoration and genetic diversity of drought-tolerant breeding lines will be useful for the development of promising drought-tolerant rice hybrids. The extent of genetic variation of 204 drought-tolerant breeding lines based on the coefcient of parentage (COP) and test crossing with IR 58025A is studied. The linked molecular markers to Rf genes were screened on identifed maintainers and restorers. The average value of COP for 204 drought-tolerant breeding lines was 0.21, which indicates genetic divergence in breeding lines. Four major groups comprising of 81 IR 64 type, 37 Apo type, 28 Vandana type, 6 WAB type and 52 others were identifed. Out of 204 breeding lines, 37 (18.14%) maintainers, 55 (26.96%) partial maintainers, 63 (30.88%) partial restorers and 49 (24.02%) restorers were identifed. Rf genes linked molecular markers RM7466 and RM443 on chr. 1; RM6344 on chr. 7 and RM6100, RM1108, RM216, RM171 and RM244 on chr. 10 were found associated with restorers and maintainers. These Rf genes linked molecular markers might be furthermore used in marker-assisted restorer selection in cytoplasmic male sterile-WA system from large source nurseries to avoid usual test crosses in the hybrid rice development programme. The identifed restorer and maintainers may be used for the development of high-yielding drought-tolerant rice hybrids.

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        Obstetric outcome of induction of labor using prostaglandin gel in patients with previous one cesarean section

        Vijayata Sangwan,Sunita Siwach,Pinki Lakra,Mukesh Sangwan,Sanjeet Singh,Rajiv Mahendru 대한산부인과학회 2019 Obstetrics & Gynecology Science Vol.62 No.6

        ObjectiveAfter globally acceptance of planned vaginal birth after cesarean section (VBAC), the mode of induction is still amatter of debate and requires further discussion. We aimed to study obstetric outcomes in post-cesarean patientsundergoing induction of labor with prostaglandin gel compared with patients who developed spontaneous laborpains. MethodsAll patients at 34 weeks or more of gestation with previous one cesarean section eligible for trial of labor aftercesarean section admitted in a labor room within one year were divided in 2 groups. Group one consisted of patientswho experienced the spontaneous onset of labor pains and group 2 consisted of patients who underwent inductionof labor with prostaglandin gel. They were analyzed for maternofetal outcomes. Descriptive statistics, independentsample t-test, and chi-square test were applied using SPSS 20 software for statistical analysis. ResultsBoth groups were comparable in maternal age, parity, and fetal weight, but different in bishop score, mode ofdelivery, and neonatal outcome. Admisson bishop score was 6.61±2.51 in group 1 and 3.15±1.27 in group 2 (P<0.005). In the patients who experienced spontaneous labor, 86.82% had successful VBAC. In the patients with induced labor,64.34% had successful VBAC with an average dose of gel of 1.65±0.75. Both groups had one case each of uterinerupture. The neonatal intensive care unit admission rate was 4.1% in group one and 10.4% in group 2. ConclusionThis study reflects that supervised labor induction with prostaglandin gel in previous one cesarean section patients isa safe and effective option.

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