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Jae Su Kim,Se Jin Lee,Jong Chul Kim,Sihyeon Kim,Mi Rong Lee,Yi-Ting Yang 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
Western flower thrips, (Pergande) (Thysanoptera: Thripidae) have been recognized as serious pests worldwide. Commercially available chemical pesticides challenges against the resistance of the pest and safety to consumers. Herein we investigated the usability of soil-treatment of entomopathogenic Beauveria bassiana (Bb) ERL isolates in tomatoes. Bb-egfp was highly virulent to western flower thrips in laboratory conditions and colonized in soil, which provided a chance for western flower thrips larvae to contact the colonized fungal mass. In a glasshouse trial, Bb ERL836 and Bb ERL1578 granule (GR) were applied to soil surface and mixed well, and five females were infested to each pot of tomato 3 days after the application. The two treatments significantly reduced thrips population in tomatoes 20 and 40 days post applications, comparable to the control efficacy of clothianidin granule (GR) formulation. In the following field trial, the two ERL fungal treatments showed 69.2% and 61.5% of control efficacy, which were similar to that of clothianidin GR (76.9%). This work suggests that applied B. bassiana colonized in soil well and thrips larvae easily contacted the fungal mass, which possibly enabled the control strategy to be alternative way to the chemical control.
Perspective of entomopathogenic fungi as biological control agents
Jae Su Kim,Se Jin Lee,Jong Chul Kim,Sihyeon Kim,Jeoung Seon Yu,Min Ho Song,Mi Long Lee,Yi-Ting Yang,Yu-Shin Nai,Jeoung Jun Kim,Yeon Ho Je 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
The present issue of insect resistance and environmental toxicity of pesticides is triggering deep discussion about the pest management tactics, in which pest monitoring and control activity are mainly involved. Novel control agents, hopefully overcoming the present issues and problems, should be researched and commercially applied to the farm fields. With the monitoring-based research, additionally we have to focus on the control-based, particularly control agent-based research and application. Entomo- pathogenic fungi can used as one of the possible novel control agents once considerations are given to the control of soil- or water-dwelling pests. In our research group, the entomopathogenic fungal library has been constructed using the mealworm-based isolation system, which showed a variety of opportunities of their use in pest control. Important key production technologies including granular formulation have been developed to increase their industrialization. Some entomopathogenic fungal isolates showed high biological performance in the control of rice weevils, western flower thrips and Japanese bettles in field stands. To elucidate the fungal mode of action, a fungal transformation system using AtMT and gene identification tools were established. Recently a more deep study about the relationship between insect and entomopathogenic fungi is be investigated using RNA seq. We suggest that to make the entomopathogenic fungal products be applied to agricultural farm field, R&D of down-stream process should be seriously considered as the key step.
Sihyeon Kim,Se Jin Lee,Yi-Ting Yang,Jae Su Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
Bean bug, Riptortus pedestris (Hemiptera: Alydidae) is an agriculturally serious pest in East Asian countries. Chemical pesticides have been contributed to the management of the pest, but nowadays insect resistance limits the use of chemical pesticides, thus alternatively new pesticides with different mode of actions such as entomopathogenic fungi are considered. Herein entomopathogenic Beauveria bassiana JEF isolates were collected, identified and assayed against bean bugs in laboratory conditions. Some isolates showed >80% virulence by contact-exposure and spray methods. The Agrobacterium tumefaciens-mediated transformation of B. bassiana JEF-007 generated random transformants and some mutants showed reduced virulence against Tenebrio molitor (Coleoptera: Tenebrionidae) larvae and R. pedestris nymph. Compared to the wild-type, the two transformants showed remarkably different morphology, conidial production, and thermotolerance. To figure out pathogenicity-related genes, thermal asymmetric interlaced (TAIL) PCR of the random transformants was performed and possibly some virulence-related genes were predicted. This work can be a strong platform for the functional genetics of bean bug-pathogenic B. bassiana.
Sihyeon Kim,Se Jin Lee,Mi Rong Lee,Jong Cheol Kim,So Eun Park,Jae Su Kim 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04
Beauveria bassiana (Bb) is a potential biopesticide against western flower thrips. To understand molecular characteristics of two Bb isolates (ERL836 and JEF-007), whole genome sequencing and RNA-seq were performed. The whole genome of Bb ERL836 was sequenced (3,934,801,443 bp) using Pacbio sequencing and 15 contigs were analyzed. To understand the molecular mechanisms of pathogenicity in two Bb genome (before infection and after infection), next generation sequencing (NGS) was analyzed to compare transcriptomes. A result of differentially expressed gene (DEG) showed that 2,689 contigs were up-regulated and 2,619 contigs were down-regulated. Randomly selected five genes were validated to be highly up-regulated. This work can provide an understanding of the interaction between Bb and westetn flower thrips.
hpRNA-induced gene silencing in Beauveria bassiana JEF-007 for gene characterization
Sihyeon Kim,Se Jin Lee,Mi Rong Lee,Jong Cheol Kim,So Eun Park,Jae Su Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10
A genus of entomopathogenic Beaveria bassiana has been widely used in pest management, however little studies havebeen given to its virulence-related genes. To identify the roles of virulence genes, AtMT-induced random mutants weregenerated and followed by localization study with TAIL-PCR. Two genes, Complex I intermediated-associated protein30 (CIP30) and Autophagy protein 22 (Atg22), were predicted as virulence-related genes in B.bassiana JEF-007. To validatethe a possible relationship between two genes and fungal virulence, hpRNAi was performed. A hpRNAi plasmid wasconstructed as a model system to knock down of egfp gene in egfp-expressing B. bassiana transformant. A Real-TimePCR showed the knock down of egfp gene expression via hpRNAi. The CIP30 and Atg22 genes in B. bassiana willbe validated by the established RNAi technique. This work can be a strong platform for the functional genetics in B.bassiana.
Establishment of entomopathogenic fungal virulence assay against Protaetia brevitarsis seulensis
Sihyeon Kim,Seok Ju Lee,So Eun Park,Jong Cheol Kim,Mi Rong Lee,Se Jin Lee,Jae Su Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04
Many turfgrass pests, Ectinohoplia rufipes, Exomala orientalis and Popillia quadriguttata, cause serious damage to grass fields in Korea. In this study, control of turfgrass pests using entomopathogenic fungi were investigated using Protaetia brevitarsis seulensis as an alternative insect. In a preliminary experiment, some Metathizium sp. showed ca. 60% virulence after 7 days exposure to the entomopathogenic fungi. Compared to the contact-exposure, overall a spray treatment showed lower control efficacy against P. brevitarsis seulensis. Furthermore, mortality of various developmental stages of P. brevitarsis seulensis was investigated under laboratory conditions. This work suggests that when treated with fungi mortality of P. brevitarsis seulensis was closely related with developmental stage. These results provide a basic information for the control of P. brevitarsis seulensis using entomopathogenic fungi
Sihyeon Kim,Jong Cheol Kim,Mi Rong Lee,So Eun Park,Dongwei Li,Sehyeon Baek,Laila Gasmi,Min Sung Jo,Tae Young Shin,Junheon Kim,Jae Su Kim 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
Insect killing fungus Beauveria bassiana has been widely studied as a biological control agent. However, many studies have been focused on lab or field-based management. Herein this work, comparison of three B. bassiana strains was investigated under a molecular level. The whole genome sequences of ERL836, JEF-007 were analyzed by PacBio (35.5 Mb of ERL836 and 36.5 Mb of JEF-007) and ARSEF2860 referenced from GenBank (33.7 Mb). To compare the three strains, virulence, thermotolerance and chemical resistance were assayed. The transcriptomes of non-infecting B. bassiana and infecting B. bassiana against western flower thrips were analyzed using RNA-seq. This work can provide that genome features, functions, morphology and gene expression could be different under the molecular level, even if in the same species.
( Sihyeon Kim ),( Jong Cheol Kim ),( Se Jin Lee ),( Mi Rong Lee ),( So Eun Park ),( Dongwei Li ),( Sehyeon Baek ),( Tae Young Shin ),( Laila Gasmi ),( Jae Su Kim ) 한국균학회 2020 Mycobiology Vol.48 No.2
Root-feeding Scarabaeidae, particularly white grubs are considered among the most harmful coleopteran insect pests in turfgrass. In this work, sixteen entomopathogenic fungal species were assayed against flower chafer beetle, Protaetia brevitarsis (Coleoptera: Scarabaeidae) and Metarhizium anisopliae JEF-314 showed high virulence. The control ability of the isolate JEF-314 has been in detail tested for a model insect flower chafer beetle. Further analyses showed insect stage-dependent virulence where the fungal virulence was the highest against smaller instar larvae. Additionally, we confirmed that millet-based solid cultured granule was effective against the soil-dwelling larval stage. The isolate also showed a similar ability for a representative pest (Popillia spp.) in laboratory conditions. Our results clearly suggest a high potential of M. anisopliae JEF-314 to control the flower chafer beetle, possibly resulting in controlling of root-feeding white grubs in turfgrass. Based on the insect life cycle and susceptibility to the fungus, late spring and summer time would be the optimum time to apply JEF-314 granules for an effective control. Further characterization of the efficacy of the fungus under field conditions against the Scarabaeidae beetles might provide an efficient tool to control this beetle in an environment-friendly way.