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      • KCI등재

        Comparative Genomic Analysis and Rapid Molecular Detection of Xanthomonas euvesicatoria Using Unique ATP-Dependent DNA Helicase recQ, hrpB1, and hrpB2 Genes Isolated from Physalis pubescens in China

        Faisal Siddique,Yang Mingxiu,Xu Xiaofeng,Ni Zhe,Haseeb Younis,Peng Lili,Zhang Junhua 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.2

        Ground cherry (Physalis pubescens) is the most prominent species in the Solanaceae family due to its nutritional content, and prospective health advantages. It is grown all over the world, but notably in northern China. In 2019 firstly bacterial leaf spot (BLS) disease was identified on P. pubescens in China that caused by both BLS pathogens Xanthomonas euvesicatoria pv. euvesicatoria resulted in substantial monetary losses. Here, we compared whole genome sequences of X. euvesicatoria to other Xanthomonas species that caused BLS diseases for high similarities and dissimilarities in genomic sequences through average nucleotide identity (ANI) and BLAST comparison. Molecular techniques and phylogenetic trees were adopted to detect X. euvesicatoria on P. pubescens using recQ, hrpB1, and hrpB2 genes for efficient and precise identification. For rapid molecular detection of X. euvesicatoria, loop-mediated isothermal amplification, polymerase chain reaction (PCR), and real-time PCR techniques were used. Whole genome comparison results showed that the genome of X. euvesicatoria was more closely relative to X. perforans than X. vesicatoria, and X. gardneri with 98%, 84%, and 86% ANI, respectively. All infected leaves of P. pubescens found positive amplification, and negative controls did not show amplification. The findings of evolutionary history revealed that isolated strains XeC10RQ, XeH- 9RQ, XeA10RQ, and XeB10RQ that originated from China were closely relative and highly homologous to the X. euvesicatoria. This research provides information to researchers on genomic variation in BLS pathogens, and further molecular evolution and identification of X. euvesicatoria using the unique target recQ gene through advance molecular approaches.

      • KCI등재

        Performance Characterization and Pressure Prediction of Compression Socks

        Hafiz Faisal Siddique,Adnan Ahmed Mazari,Antonin Havelka,Zdenek KUS 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3

        Objective of the current research is to develop the mathematical model for the prediction of compression pressureby incorporating some of new but missing variables; circumferential aspect of leg (Lc) and socks (Sc), true stress (σT), true/logarithm strain (εT), true modulus of elasticity (ET), deformed width (Wf) at b ankle position. Socks samples were purchased,hand washed, donned, marked, sliced to loops and then cut to rectangular strips to obtain force-elongation curve at differentextensions context to practical elongation. Additionally, all samples were evaluated to analyze the influnce of multiplemachinewashes as well as for identification of most influential fabric parameter on compresson pressure, statistically. Standard sized wooden leg (S9=24 cm) attached with pneumatic based sensors (Salzmann MST MK IV) to medial side wasused. Force-extension diagram was obtained using Testometric tensile tester. Mathematical models were developed on thebasis of principle of Laplace’s law. Developed two models were analyzed and comapred with two existing recently developedmodels statitistically using MINTAB 17 software.

      • KCI등재

        Performance Characterization of Compression Socks at Ankle Portion under Multiple Mechanical Impacts

        Hafiz Faisal Siddique,Adnan Ahmed Mazari,Antonin Havelka,Zdenek KUS 한국섬유공학회 2019 Fibers and polymers Vol.20 No.5

        The aim of this research is to investigate how performance of compression socks is deteriorated due to expectedinduced wearing mechanical impacts. Wearing mechanical impacts influence the durability parameters i.e. tensile energyloss, extent of pressure deterioration and dimensional deformation. This research is comprised of two parts; 1): Comparisonof unwashed and hand washed cut-strips for tensile energy and dimensional deformation. For tensile energy loss, cut-stripsamples were-interacted to constant rate of loading and un-loading, cyclic-loading upto 15th cycles for ±5 mm extension(considering muscles expansion and relaxation) and were dwelled (stayed) for 3 minutes at 25 %, 50 % and 75 % extensionlevels, simultaneously. As a second part 2); Three levels (1st, 2nd and 3rd) of gradual intensive machine washes wereperformed to quantify the extent of deterioration of compression pressure, worn alternatively on a fixed sized plastic leg,using KIKUHIME pressure measuring device. Dimensional deformation due to hand washing while dimensional recovery;immediately after pressure measurement (after Pr.), and a-day-later after pressure measurement (dL Pr.) was compared andstatistically analyzed. Statistical validation of second part was performed by introducing measures of correlation, p-value(≤ 0.05), R-square values using MINITAB 17 software.

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        Memristor Based Full Adder Circuit for Better Performance

        Muhammad Khalid,Sana Mukhtar,Mohammad Jawaid Siddique,Sumair Faisal Ahmed 한국전기전자재료학회 2019 Transactions on Electrical and Electronic Material Vol.20 No.5

        In this paper, we have designed memristor based AND, OR, and exclusive-OR (XOR) gates. With the help of these gates, memristor based full adder circuit has proposed. Simulation results of the proposed circuit including all above gates have been reported. Prominent improvement of the proposed circuit has been represented in power consumption 54.74%, delay 14.84%, and lesser transistor count with respect to conventional circuit.

      • KCI등재

        Adrenergic blocker terazosin potentially suppresses acetaminophen induced-acute liver injury in animal models via CYP2E1 gene

        Hashmat Zoya,Channa Iffat Saeed,Safdar Muhammad,Ozaslan Mehmet,Saeed Muhammad,Siddique Faisal,Junejo Yasmeen 한국독성학회 2022 Toxicological Research Vol.38 No.3

        Drug induced liver injury (DILI) is a global issue and acetaminophen (APAP) is considered as the main cause of this. Due to increasing incidents of DILI, current study attempted to investigate an alternative but better role of terazosin (alpha-adrenergic blocker) in APAP-induced acute liver injury in an animal model using New Zealand rabbits. APAP (1 g/kg of body weight) was given to New Zealand rabbits either with or without terazosin (0.5 mg/kg) and serum was collected after 4 h. Serum alanine transaminase (ALT), alkaline phosphatase (ALP) and ferritin level were determined to analyze the liver functioning of treated rabbits. Furthermore, total cholesterol (TC), total lipids (TL), high-density lipoproteins (HDL), low-density lipoprotein (LDL) and triglycerides (TG) levels were estimated to find any change in lipid profile of the treated animals. Moreover, the urea and creatinine levels assayed the actual renal functionality. To identify any modification in gene expression, qPCR of cytochrome P2E1 (CYP2E1) was performed. Terazosin in combination with APAP enhanced liver functioning by reducing the levels of liver injury markers viz. ALP and ALT, while lipid profile was also lowered by down regulation of TC, TL, LDL and TG with enhanced HDL levels. It caused significant down regulation of expression level of CYP2E1. It is concluded that terazosin has better effects induced on the recovery of normal liver functioning, by improving the liver profile, lipid profile and renal functioning both at tissue and molecular levels.

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