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- 저자 : Shanda Wang (검색결과 4 건)
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Shanda Liu,Xiaoping Wang,Eryang Li,Carl J. Douglas,Jin-Gui Chen,Shucai Wang 한국식물학회 2013 Journal of Plant Biology Vol.56 No.4
R2R3 MYB transcription factors regulate multiple aspects of plant growth and development. Here we report the identification of PtrMYB192, a Populus R2R3 MYB transcription factor, as a negative regulator of flowering time. By using quantitative RT-PCR, we found that PtrMYB192, but not its closely homologous gene PtrMYB028, is highly expressed in mature leaves in Populus. Heterologously expression of PtrMYB192 under control of 35S promoter in Arabidopsis resulted in late flowering phenotypes under both long and short day conditions, indicating that PtrMYB192 controls flowering time independent of the photoperiod pathway. Domain swapping experiment showed that neither PtrMYB028DB-192AD nor PtrMYB192DB-028AD affected flowering time when heterologously expressed in Arabidopsis. However, when recruit to the promoter of a GAL4-GUS reporter gene by a GAL4 DNA binding domain in Arabidopsis protoplasts, both of PtrMYB028DB-192AD and PtrMYB192DB-028AD activated the reporter gene. Quantitative RT-PCR results showed an elevated expression of the floral repressor gene FLOWERING LOCUS C (FLC), but not the flowering-promoting gene CONSTANS (CO) in PtrMYB192 transgenic plants. Taken together, these results suggest that PtrMYB192 is a transcription activator that negatively regulating flowering time in Arabidopsis by activating FLC and possible other genes, and that both R2R3 DNA binding domain and activation domain maybe required for its full function.
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Qingnan Hu,Li Yang,Shanda Liu,Limei Zhou,Xutong Wang,Wei Wang,Ling Cai,Xiuju Wu,Ying Chang,Shucai Wang 한국식물학회 2016 Journal of Plant Biology Vol.59 No.5
Single-repeat R3 MYB transcription factors (R3 MYBs) regulate epidermal cell fate determination in Arabidopsis through a lateral inhibition mechanism. Previously we have shown that poplar R3 MYB genes regulate trichome formation when expressed in Arabidopsis. Here we report the identification and functional characterization of a poplar R3 MYB-like protein, Populus trichocarpa R3 MYB-LIKE1 (PtrRML1). So far all the MYB transcription factors identified have a highly conserved N-terminal DNA-binding domain composed of MYB repeats and a highly variable C-terminal domain. However, PtrRML1 has a single R3 MYB repeat at its C-terminal and an LxLxL repressor motif-containing N-terminal, and with amino acids about twice of that of the known R3 MYBs. PtrRML1 is localized in nuclear in transgenic Arabidopsis plants expressing PtrRML1-GFP. PtrRML1 repressed reporter gene expression in protoplasts, and it interacted with GL3 in plant cells. Expression of PtrRML1 in Arabidopsis resulted in glabrous phenotypes, increased number of root hairs, and decreased anthocyanin accumulation. Consist with these observation, the expression levels of some MBW component genes and some of their target genes involved in the regulation of epidermal cell fate determination and anthocyanin biosynthesis, including TRY, CPC, ETC1, GL1, GL2, DFR, UF3GT and TT8 were reduced in the transgenic plants.
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The Effect of Ultrasonic Additive Manufacturing on Integrated Printed Electronic Conductors
Alkaios Bournias-Varotsis,Shanda Wang,David Hutt,Daniel S. Engstrøm 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.4
Ultrasonic additive manufacturing (UAM) is a low temperature manufacturing method capable of embedding printed electronicsin metal components. The eff ect of UAM processing on the resistivity of conductive tracks printed with fi ve diff erentconductive pastes based on silver, copper or carbon fl akes/particles in either a thermoplastic or thermoset fi ller binder areinvestigated. For all but the carbon-based paste, the resistivity changed linearly with the UAM energy input. After UAMprocessing, a resistivity increase of more than 150 times was recorded for the copper based thermoset paste. The silver basedpastes showed a resistivity increase of between 1.1 and 50 times from their initial values. The carbon-based paste showedno change in resistivity after UAM processing. Focussed ion beam microstructure analysis of the printed conductive tracksbefore and after UAM processing showed that the silver particles and fl akes in at least one of the pastes partly dislodgedfrom their thermoset fi ller creating voids, thereby increasing the resistivity, whereas the silver fl akes in a thermoplastic fi llerdid not dislodge due to material fl ow of the polymer binder. The lowest resistivity (8 × 10 −5 Ω cm) after UAM processingwas achieved for a thermoplastic paste with silver fl akes at low UAM processing energy.
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Yulan Gao,Xiangchen Fang,Zhenmin Cheng,Liming Xu,Zhenhui Lu,Shanda Wang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.26 No.-
An ex-situ presulfurization technology for transforming the metal oxides of hydrotreating catalyst to the corresponding metal oxy-sulfides was developed, involving pre-dispersion of an organo-nitrogen substance onto catalyst and a subsequent multi-step heat treatment. The inherence of exothermic effect involved in the ex situ pre-sulfurized catalyst fabrication and activation, and particularly the relationship between the exothermic effect and the surface property of catalyst were investigated. The results indicated that both the preparation parameters and the operating conditions can have effects on the exothermic behavior and surface state of the catalyst. The information obtained in the current study is useful for optimizing the EPRES process, essential for hydrotreating catalyst and its application.
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