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K. H. Seo,K.M.Lee 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.6
This study examined the effects of the H2 flow rate and the substrate temperature on the structural, electrical, and opticalcharacteristics of the IZO thin films intended for use as anode materials in OLEDs (organic light emitting diodes). These IZOthin films were deposited by RF magnetron sputtering at room temperature and 300 oC at various H2 flow rates. To examinethe effects of H2, the H2 flow rate in the argon mixing gas was changed from 0.1 sccm to 0.9 sccm. IZO thin films depositedat room temperature showed an amorphous structure, whereas IZO thin films deposited at 300 oC showed a crystallinestructure with an (222) preferential orientation regardless of the H2 flow rate. The electrical resistivity of the IZO thin filmsdecreased with increasing H2 flow rate under Ar+H2. The change in the electrical resistivity with increasing H2 flow rate wasinterpreted mainly in terms of the charge carrier concentration rather than the charge carrier mobility. The electricalresistivity of the amorphous-IZO films deposited at R.T. was lower than that of the crystalline-IZO thin films deposited at300 oC. All the films showed an average transmittance of more than 83% in the visible range. The optical band gap of the IZOfilms increased with increasing H2 flow rate. The current density and luminance of the OLED devices with IZO thin filmsdeposited at room temperature in 0.9 sccm H2 ambient gas were the highest among the films examined. These properties wereattributed to the improved optical band gap, which plays a major role in the OLED device performance.
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Kim, J.Y.,Kim, K.B.,Son, H.J.,Chae, Y.C.,Oh, S.T.,Kim, D.W.,Pak, J.H.,Seo, S.B. North-Holland Pub ; Elsevier Science Ltd 2012 FEBS letters Vol.586 No.19
Significant progress has been made in understanding the relationship between histone modifications and 'reader' molecules and their effects on transcriptional regulation. A previously identified INHAT complex subunit, SET/TAF-Iβ, binds to histones and inhibits histone acetylation. To investigate the binding specificities of SET/TAF-Iβ to various histone modifications, we employed modified histone tail peptide array analyses. SET/TAF-Iβ strongly recognized PRC2-mediated H3K27me½/3; however, the bindings were completely disrupted by H3S28 phosphorylation. We have demonstrated that SET/TAF-Iβ is sequentially recruited to the target gene promoter ATF3 after the PRC2 complex via H3K27me recognition and may offer additive effects in the repression of the target gene.
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SEO, J. H.,KIM, K. H.,KIM, H. Wiley (Blackwell Publishing) 2007 Annals of the New York Academy of Sciences Vol.1096 No.1
<P>Proteinase-activated receptor-2 (PAR-2) belongs to a novel subfamily of G protein-coupled receptors with seven-transmembrane domains. PAR-2 is activated by serine proteases, such as trypsin, mast cell tryptase, and allergic or bacterial proteases. The presence of trypsin has been shown in human stomach. Cyclooxygenase-2 (COX-2) is induced by inflammatory cytokines, growth factors, gastrin, and reactive oxygen species in gastric epithelial cells, which may lead to mutagenesis and subsequent metaplasia, dysplasia, and cancer formation. We investigated whether PAR-2 is activated in H. pylori (HP99)-infected cells, which is related to COX-2 induction in gastric epithelial cells. After treatment of H. pylori to AGS (gastric adenocarcinoma) cells at a bacteria/cell ratio of 100:1, we determine the expression and the activation of PAR-2 and the expression of COX-2. The same experiments were performed in the cells treated with PAR-2 agonist peptide. mRNA and protein expression of PAR-2 and COX-2 were determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. PAR-2 activation was assessed by increase in intracellular calcium level. As a result, H. pylori induced the activation and expression of PAR-2 as well as COX-2 expression. PAR-2 agonist peptide augmented H. pylori-induced COX-2 expression in AGS cells. H. pylori induces COX-2 expression, which is mediated by both activation and expression of PAR-2 in gastric epithelial cells.</P>
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Transmissibility of novel H7N9 and H9N2 avian influenza viruses between chickens and ferrets
Ku, K.B.,Park, E.H.,Yum, J.,Kim, H.M.,Kang, Y.M.,Kim, J.C.,Kim, J.A.,Kim, H.S.,Seo, S.H. 3M Company 2014 Virology Vol.450 No.-
Previous studies have shown that the H7N9 avian influenza virus cannot be transmitted efficiently between ferrets via respiratory droplets. Here, we studied the infectivity of the H7N9 avian influenza virus in chickens and its transmissibility from infected to naive chickens and ferrets. The H7N9 virus (A/Anhui/½013) replicated poorly in chickens and could not be transmitted efficiently from infected chickens to naive chickens and ferrets. H7N9 virus was shed from chicken tracheae for only 2 days after infection and from chicken cloacae for only 1 day after infection, while the H9N2 avian influenza virus, which is endemic in chickens in many Asian countries, was shed from tracheae and cloacae for 8 days after infection. Taken together, our results suggest that chickens may be a poor agent of transmission for the H7N9 virus to other chickens and to mammals, including humans.
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Lee, K. H.,Kang, S. K.,Goo, J. M.,Lee, J. S.,Cheon, G. J.,Seo, S.,Hwang, E. J. INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH 2017 Anticancer research Vol.37 No.3
<P>Background/Aim: To compare the relationship between Ktrans from DCE-MRI and K1 from dynamic (NNH3)-N-13- PET, with simultaneous and separate MR/PET in the VX-2 rabbit carcinoma model. Materials and Methods: MR/PET was performed simultaneously and separately, 14 and 15 days after VX-2 tumor implantation at the paravertebral muscle. The Ktrans and K-1 values were estimated using an in-house software program. The relationships between Ktrans and K-1 were analyzed using Pearson's correlation coefficients and linear/non-linear regression function. Results: Assuming a linear relationship, Ktrans and K-1 exhibited a moderate positive correlations with both simultaneous ( r=0.54-0.57) and separate ( r=0.53-0.69) imaging. However, while the Ktrans and K-1 from separate imaging were linearly correlated, those from simultaneous imaging exhibited a non-linear relationship. The amount of change in K-1 associated with a unit increase in Ktrans varied depending on Ktrans values. Conclusion: The relationship between K-trans and K-1 may be mis-interpreted with separate MR and PET acquisition.</P>
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Kim, E-K,Seo, H-S,Chae, M-J,Jeon, I-S,Song, B-Y,Park, Y-J,Ahn, H M,Yun, C-O,Kang, C-Y Macmillan Publishers Limited 2014 Gene therapy Vol.21 No.1
For successful clinical tumor immunotherapy outcomes, strong immune responses against tumor antigens must be generated. Cell-based vaccines compromise one strategy with which to induce appropriate strong immune responses. Previously, we established a natural killer T-cell (NKT) ligand-loaded, adenoviral vector-transduced B-cell-based anticancer cellular vaccine. To enhance tumor antigen delivery to B cells, we established a modified adenoviral vector (Ad-k35) that encoded a truncated form of the breast cancer antigen Her2/neu (Ad-k35HM) in which fiber structure was substituted with adenovirus serotype 35. We observed increased tumor antigen expression with Ad-k35HM in both human and murine B cells. In addition, an Ad-k35HM-transduced B-cell vaccine elicited strong antigen-specific cellular and humoral immune responses that were further enhanced with the additional loading of soluble NKT ligand KBC009. An Ad-k35HM-transduced, KBC009-loaded B-cell vaccine efficiently suppressed the in vivo growth of established tumors in a mouse model. Moreover, the vaccine elicited human leukocyte antigen (HLA)-A2 epitope-specific cytotoxic T-cell responses in B6.Cg (CB)-Tg (HLA-A/H2-D) 2Enge/Jat mice. These findings indicated that the Ad-k35 could be appropriate for the preclinical and clinical development of B-cell-based anticancer immunotherapies.
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Kim, J.H.,Kim, T.H.,Park, H.J.,Choi, Y.J.,Kang, J.H.,Song, K.H.,Koo, T.S.,Seo, K.W. Ballière Tindall ; W.B. Saunders 2016 The veterinary journal Vol.208 No.-
<P>Tulobuterol is a beta(2)-adrenergic agonist that was the first bronchodilator approved as a transdermal patch for humans. Previous studies have examined the pharmacokinetics of tulobuterol in humans but not in the veterinary species. In this study, the pharmacokinetics of tulobuterol was examined in healthy Beagle dogs after transdermal and intravenous administration. The C-max was 2.09 ng/mL at 16.0 h for a 0.2 mg/kg patch and 4.85 ng/mL at 13.6 h for a 0.4 mg/kg patch. The effective blood level in humans is 1-3 ng/mL, a concentration achieved using the 0.2 mg/kg patch in dogs. In conclusion, application of a 0.2 mg/kg tulobuterol patch to healthy dogs led to an apparently effective blood concentration for 24 h. (C) 2015 Elsevier Ltd. All rights reserved.</P>
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고온기 초지의 예취관리에 관한 연구 6 . 고온기간중 예취높이와 질소 및 칼리추비가 혼파초지의 식생과 수량 및 목초품질에 미치는 영향
서성(S . Seo),조무환(M . H . Jo),박문수(M . S . Park),이종경(J . K . Lee),한영춘(Y . C . Han) 한국축산학회 1988 한국축산학회지 Vol.30 No.8
A field experiment was carried out to determine the effects of cutting height at the third cut and application of N and K₂O after cutting during mid-summer season on the soil temperature, plant height, grass coverage, percentages of grass and legume, yield and grass quality in orchardgrass dominated pasture. With three cutting heights (3, 6 and 9㎝) and 4 fertilizer levels (0, N 50㎏, K₂O 50㎏, and N 50㎏ + K₂O 50㎏ ㏊^(-1)), present experiment was conducted at Livestock Experiment Station, Suwon in 1987. 1. The soil temperature after the third cut was rapidly elevated, and lower value was observed with 9㎝ of cutting height through the regrowth period. Plant height after cutting was remarkably increased with higher stubble height regardless of fertilizer levels. 2. The grass coverage and percentage of grass were increased as stubble height was high, while the influence of fertilizer on those was very little. Application of N and K₂O with lower stubble height significantly increased the percentage of bareland. 3. The yield at the third cut was decreased with higher height. But the regrowth yield was significantly increased as the cutting height was increased regardless of fertilizer levels. Regrowth yield was great at N 50㎏ + K₂O 50㎏ $gt; 0 $gt; K₂O 50㎏, N 50㎏, in that order. However, there were not significantly different on regrowth yield among fertilizer treatment. 4. The contents of crude protein, crude fat, crude ash, P, K and Ca were decreased as stubble height was high, and that of crude fiber was increased at 9 ㎝ cutting height. Also the influence of N and K₂O fertilizer on grass quality was little.
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Lee, S.J.,Yoon, J.W.,Seo, Y.K.,Kim, M.B.,Lee, S.K.,Lee, U.H.,Hwang, Y.K.,Bae, Y.S.,Chang, J.S. Elsevier 2014 Microporous and mesoporous materials Vol.193 No.-
The effect of purification condition of a chromium terephthalate MIL-101 on gas storage (H<SUB>2</SUB>, CO<SUB>2</SUB>, and C<SUB>2</SUB>H<SUB>2</SUB>) and C2 hydrocarbon separations was investigated for MIL-101a, MIL-101b, and MIL-101c, which were obtained by three-step sequential purification using hot water/ethanol and aqueous NH<SUB>4</SUB>F solution treatments. MIL-101c was the best in terms of gas storage capacities among the three samples possibly due to the removal of free and coordinated terephthalic acids. Remarkably, MIL-101c exhibits among the highest total hydrogen uptake at 50bar and 77K (9.9 wt%) and shows considerably large deliverable capacity between 1.5 and 50bar (7.6 wt%). Moreover, MIL-101c shows among the highest C<SUB>2</SUB>H<SUB>2</SUB> storage capacity (6.4mmolg<SUP>-1</SUP>) and deliverable capacity (4.3mmolg<SUP>-1</SUP>) at 1bar and 313K. For C<SUB>2</SUB>H<SUB>2</SUB>/C<SUB>2</SUB>H<SUB>6</SUB>, C<SUB>2</SUB>H<SUB>2</SUB>/C<SUB>2</SUB>H<SUB>4</SUB>, and C<SUB>2</SUB>H<SUB>4</SUB>/C<SUB>2</SUB>H<SUB>6</SUB> separations, MIL-101c exhibits higher adsorption capacities, working capacities and adsorption figure of merit (AFM) values while MIL-101b shows higher selectivities and sorbent selection parameters. These results clearly show that a proper purification of MIL-101 is important for gas storage and gas separations.
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Photochemical tuning of ultrathin TiO2/p-Si p-n junction properties via UV-induced H doping
Lee, S. Y.,Kim, J.,Ahn, B.,Cho, I. S.,Yu, H. K.,Seo, H. Springer Science + Business Media 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.2
<P>We report a modified TiO2/p-Si electronic structure that uses ultraviolet exposure for the incorporation of H. This structure was characterized using various photoelectron spectroscopic techniques. The ultraviolet (UV) exposure of the TiO2 surface allowed the Fermi energy level to be tuned by the insertion of H radicals, which induced changes in the heterojunction TiO2/p-Si diode properties. The UV exposure of the TiO2 surface was performed in air. On UVexposure, a photochemical reaction involving the incorporation of UV-induced H radicals led to the creation of a surface Ti-O-OH group and caused interstitial H doping (Ti-H-O) in the bulk, which modified the electronic structures in different ways, depending on the location of the H. On the basis of the band alignment determined using a combined spectroscopic analysis, it is suggested that the UV-induced H incorporation into the TiO2 could be utilized for the systematic tuning of the heterojunction property for solar cells, photocatalytic applications, and capacitors.</P>
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