<i>MicroRNA-375</i> Functions as a Tumor-Suppressor Gene in Gastric Cancer by Targeting Recepteur d’Origine Nantais

Lian, Sen,Park, Jung Sun,Xia, Yong,Nguyen, Thi Thinh,Joo, Young Eun,Kim, Kyung Keun,Kim, Hark Kyun,Jung, Young Do MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.10

<P>Emerging evidence supports a fundamental role for microRNAs (miRNA) in regulating cancer metastasis. Recently, <I>microRNA-375</I> (<I>miR-375</I>) was reported to be downregulated in many types of cancers, including gastric cancer. Increase in the expression of Recepteur d’Origine Nantais (RON), a receptor tyrosine kinase, has been reported in tumors. However, the function of <I>miR-375</I> and RON expression in gastric cancer metastasis has not been sufficiently studied. In silico analysis identified <I>miR-375</I> binding sites in the 3′-untranslated regions (3′-UTR) of the RON-encoding gene. Expression of <I>miR-375</I> resulted in reduced activity of a luciferase reporter containing the 3′-UTR fragments of RON-encoding mRNA, confirming that <I>miR-375</I> directly targets the 3′-UTR of RON mRNA. Moreover, we found that overexpression of <I>miR-375</I> inhibited mRNA and protein expression of RON, which was accompanied by the suppression of cell proliferation, migration, and invasion in gastric cancer AGS and MKN-28 cells. Ectopic <I>miR-375</I> expression also induced G1 cell cycle arrest through a decrease in the expression of cyclin D1, cyclin D3, and in the phosphorylation of retinoblastoma (Rb). Knockdown of RON by RNAi, similar to <I>miR-375</I> overexpression, suppressed tumorigenic properties and induced G1 arrest through a decrease in the expression of cyclin D1, cyclin D3, and in the phosphorylation of Rb. Thus, our study provides evidence that <I>miR-375</I> acts as a suppressor of metastasis in gastric cancer by targeting RON, and might represent a new potential therapeutic target for gastric cancer.</P>

Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides

Sen Lian,김국형,Miranda Gilda Jonson,조원경,최홍수,제연호 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.1

Rice stripe virus (RSV) is one of serious epidemic pathogens for rice species grown in many Asian countries. Therefore, it is necessary to produce a diagnostic detection kit applicable in fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic RSV polypeptides for CP, NCP, NS3 and NSvc4 we also generated and only antibodies against CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSvc4 showed weak specific bands as well as strong non-specific background due to the difference of viral proteins produced in the infected leaves. In summary,the antibodies generated against RSV proteins produced in this study will be useful for various assays such as for RSV diagnostic detection, immunoprecipitation,protein purification, and western blot analysis.

Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides

Lian, Sen,Jonson, Miranda Gilda,Cho, Won-Kyong,Choi, Hong-Soo,Je, Yeon-Ho,Kim, Kook-Hyung The Korean Society of Plant Pathology 2011 Plant Pathology Journal Vol.27 No.1

Rice stripe virus (RSV) is one of serious epidemic pathogens for rice species grown in many Asian countries. Therefore, it is necessary to produce a diagnostic detection kit applicable in fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic RSV polypeptides for CP, NCP, NS3 and NSvc4 we also generated and only antibodies against CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSvc4 showed weak specific bands as well as strong non-specific background due to the difference of viral proteins produced in the infected leaves. In summary, the antibodies generated against RSV proteins produced in this study will be useful for various assays such as for RSV diagnostic detection, immunoprecipitation, protein purification, and western blot analysis.

Impact of timely BCR-ABL1 monitoring before allogeneic stem cell transplantation among patients with BCR-ABL1-positive B-acute lymphoblastic leukemia

Siew Lian Chong,Asral Wirda Ahmad Asnawi,Tze Shin Leong,Jenq Tzong Tan,Kian Boon Law,Siong Leng Hon,Rui Jeat Fann,Sen Mui Tan 대한혈액학회 2021 Blood Research Vol.56 No.3

Background With the emergence of tyrosine kinase inhibitors and the incorporation of stringent measurable residual disease (MRD) monitoring, risk stratification for BCR-ABL1-positive acute lymphoblastic leukemia (ALL) patients has changed significantly. However, whether this monitoring can replace conventional risk factors in determining whether patients need allogeneic stem cell transplantation is still unclear. This study aimed to determine the impact of BCR-ABL1 monitoring on the outcome of patients with BCR-ABL1-positive ALL after allogeneic stem cell transplantation. Methods We retrospectively analyzed the survival outcome of patients with BCR-ABL1-positive ALL based on the quantification of BCR-ABL1 at 3 timepoints: the end of induction (timepoint 1), post-consolidation week 16 (timepoint 2), and the end of treatment for patients who were either transplant-eligible or non-transplant eligible (timepoint 3). Results From 2006 to 2018, a total of 96 patients newly diagnosed with BCR-ABL1-positive ALL were treated with chemotherapy and tyrosine kinase inhibitors. Thirty-eight (41.3%) patients achieved complete remission, and 33 patients underwent allogeneic stem cell transplantation. Our data showed that pre-transplant MRD monitoring by real-time quantitative polymerase chain reaction had the highest correlation with survival in patients with BCR-ABL1-positive ALL, especially for those who underwent allogeneic stem cell transplantation. Conclusion Patients without MRD pre-transplantation had superior survival compared with those who had MRD, and they had excellent long-term outcomes after allogeneic stem cell transplantation.

Current Insights into Research on Rice stripe virus

조원경,김국형,Sen Lian,김상민,박상호 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.3

Rice stripe virus (RSV) is one of the most destructive viruses of rice, and greatly reduces rice production in China, Japan, and Korea, where mostly japonica cultivars of rice are grown. RSV is transmitted by the small brown plant-hopper (SBPH) in a persistent and circulative-propagative manner. Several methods have been developed for detection of RSV, which is composed of four single-stranded RNAs that encode seven proteins. Genome sequence data and comparative phylogenetic analysis have been used to identify the origin and diversity of RSV isolates. Several rice varieties resistant to RSV have been selected and QTL analysis and fine mapping have been intensively performed to map RSV resistance loci or genes. RSV genes have been used to generate several genetically modified transgenic rice plants with RSV resistance. Recently, genome-wide transcriptome analyses and deep sequencing have been used to identify mRNAs and small RNAs involved in RSV infection; several rice host factors that interact with RSV proteins have also been identified. In this article, we review the current statues of RSV research and propose integrated approaches for the study of interactions among RSV, rice, and the SBPH.

Chrysin inhibits cell invasion by inhibition of Recepteur d’origine Nantais via suppressing early growth response-1 and NF-κB transcription factor activities in gastric cancer cells

XIA, YONG,LIAN, SEN,KHOI, PHAM NGOC,YOON, HYUN JOONG,HAN, JAE YOUNG,CHAY, KEE OH,KIM, KYUNG KEUN,JUNG, YOUNG DO Spandidos Publications 2015 International journal of oncology Vol.46 No.4

<P>Cell invasion is one of crucial reasons for cancer metastasis and malignancy. Recepteur d'origine Nantais (RON) has been reported to play an important role in the cancer cell invasion process. High accumulation and activation of RON has been implicated in gastric adenocarcinoma AGS cells. Chrysin is a naturally occurring phytochemical, a type of flavonoid, which has been reported to suppress tumor metastasis. However, the effects of chrysin on RON expression in gastric cancer are not well studied. In the present study, we examined whether chrysin affects RON expression in gastric cancer, and if so, its underlying mechanism. We examined the effect of chrysin on RON expression and activity, via RT-PCR, promoter study, and western blotting in human gastric cancer AGS cells. Chrysin significantly inhibited endogenous and inducible RON expression in a dose-dependent manner. After demonstrating that Egr-1 and NF-kappa B are the critically required transcription factors for RON expression, we discovered that chrysin suppressed Egr-1 and NF-K13 transcription factor activities. Additionally, the phorbol-12-myristate-13-acetate(PMA) induced cell invasion was partially abrogated by chrysin and an RON antibody. Our results suggest that chrysin has anticancer effects at least by suppressing RON expression through blocking Egr-1 and NF-kappa B in gastric cancer AGS cells.</P>

Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X

Choi, Hoseong,Jo, Yeonhwa,Lian, Sen,Jo, Kyoung-Min,Chu, Hyosub,Yoon, Ju-Yeon,Choi, Seung-Kook,Kim, Kook-Hyung,Cho, Won Kyong Springer-Verlag 2015 Plant Molecular Biology Vol.88 No.3

Phenological Characteristics of Potted Kumquat under Protected Culture

Yung-Chiung Chang,Yu-Sen Chang,Iou-Zen Chen,Lian-Hsiung Lin 한국원예학회 2020 원예과학기술지 Vol.38 No.2

This study investigated phenological changes, shoot growth, and flowering habits of potted kumquat[Fortunella margarita (Lour.) Swingle] grown in subtropical conditions of I-Lan County, Taiwan. The shoots of the second, third, and fourth flushes of kumquat sprouted in mid-May, early July, andearly September, corresponding to 70-80, 112-122, and 168-178 days after the buds sprouted fromthe first flush, respectively. As the flushing sequence progressed, the number of shoots, the rate offlowering, and the number of flowers decreased. There were four flowering peaks in 2010 and fivein 2011. The shoots of the secondary and tertiary flushes still bloomed in 2010, but they did notflower in 2011. In 2011, the second flowering period of the first flush shoot, which flowered inmid-July, fruit set, and fruit growth showed the best performance, and the whole fruit developmentduration took about 169 days.

Current Insights into Research on Rice stripe virus

Cho, Won Kyong,Lian, Sen,Kim, Sang-Min,Park, Sang-Ho,Kim, Kook-Hyung The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.3

Rice stripe virus (RSV) is one of the most destructive viruses of rice, and greatly reduces rice production in China, Japan, and Korea, where mostly japonica cultivars of rice are grown. RSV is transmitted by the small brown plant-hopper (SBPH) in a persistent and circulative-propagative manner. Several methods have been developed for detection of RSV, which is composed of four single-stranded RNAs that encode seven proteins. Genome sequence data and comparative phylogenetic analysis have been used to identify the origin and diversity of RSV isolates. Several rice varieties resistant to RSV have been selected and QTL analysis and fine mapping have been intensively performed to map RSV resistance loci or genes. RSV genes have been used to generate several genetically modified transgenic rice plants with RSV resistance. Recently, genome-wide transcriptome analyses and deep sequencing have been used to identify mRNAs and small RNAs involved in RSV infection; several rice host factors that interact with RSV proteins have also been identified. In this article, we review the current statues of RSV research and propose integrated approaches for the study of interactions among RSV, rice, and the SBPH.

Response of Shoot Growth, Photosynthetic Capacity, Flowering, and Fruiting of Potted ‘Nagami’ Kumquat to Different Regulated Deficit Irrigation

Yung-Chiung Chang,Yu-Sen Chang,Lian-Hsiung Lin 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.4

The objective of this study was to determine the level of regulated deficit irrigation (RDI) for the potted ‘Nagami’ kumquat culture. Three treatments, control, RDI 1, and RDI 2, representing 75, 55, and 35% of available soil water content, respectively, were implemented for two weeks after the elongation of the newly-sprouted shoots (the first flush) was nearly terminated. Leaf water potential, leaf photosynthetic activity, shoot vegetative growth, flowering, and fruiting were monitored to evaluate the effects of the RDI treatments. After water withholding for two weeks (stage B), trees treated with RDI 2 decreased considerably in their leaf water potential, and exhibited the highest integrated leaf water potential index, which indicated that their leaves had suffered from the highest level of stress. The net CO2 assimilation rate (Pn) and available fluorescence (Fv)/maximum fluorescence (Fm) and Fv’/Fm’ ratios also decreased substantially in RDI 2-treated trees. However, the stressed trees exhibited compensatory growth, and all growth parameters returned to normal following re-watering. RDI 2 treatment increased flowering potential of the trees, showing the highest average flowering ratio of 50%, which differed substantially from that of the control and RDI 1. No significant differences were observed between the responses of RDI 1 and control, indicating that both treatments were not severe enough to induce a visible physiological effect in trees. By contrast, RDI 2 treatment efficiently changed the shoot growth phase and increased the number of flowers on the shoots, which may be exploited as an efficient approach for kumquat flowering control.