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이장훈,David K. Reed,이해풍,Robert W. Carlson 한국응용곤충학회 1988 한국응용곤충학회지 Vol.27 No.1
감자, 가지등 가지과 작물을 가해하는 해충인 큰 28점박이 무당벌레를 1983년 1986년까지, 매년 6월부터 9월까지 채집하였고, 이들을 공격하는 기생천적의 종류, 각 기생천적에 따른 기주곤충의 령기별, 월별 기샐률이 조사되었다. Nothoser plus afissae (Watanabe), (벌목:Proctotrupidae), Uga menoni(Kerich)(벌목:수중다리 좀벌과), Pedipbius foveolatus (Crawford), (벌목:좀벌과) 등 3종류의 기생천적이 큰 28점박이 무당벌레를 공격하는 것으로 조사되었다. N. afissae는 2령~4령때 채집한 유충에 기생하는 것으로 조사되었으며, 특히 3령때 채집한 기주에서 가장 높은 기생률로 나타났으며, 유충때, 기생해서 번데기때 우화하는 U.mononi의 경우 3령-번데기때 채집한 기주에 기생된 것으로 조사되었고, 번데기 때 재칩한 기주에서 높은 기생률을 나타냈다. N.afiss와 U.mononi는 6월뷰터 9월가지 전 조사기간에 걸쳐서 기주를 공격하는 것으로 조사되었으며, N.afissde 는 조사된 다른 기생천저등에 보다 기생률이 월등이 높은 것으로 조사되었으며 U.mononi의 겨우는 7월에만 높은 기생률을 나타냈다. P.foveolatuss는 다른 두 종류의 기생천적과는 달리 다매발생하는 것으로 남부지방에서는 8월초부터 출현하나, 경기도 지역에서는 9월초에 나타나기 시작했으며 기생된 큰28점박이 유충 1개체로 부퍼 평균 13.8개체의 성충이 우화하는 것으로 조사되었다. Henosepilachna vigintioctomaculata (Motschulsky) (Coleoptera: Coccinellidae), important pests of agricultural crops in Korea, including eggplant and potato, were collected in the Kyonggido area each summer from 1983 to 1986. Data were recorded for rates of parasitism against each host stage and seasonal activity. The parasitoids NothoserPhus afissae (Watanabe), (Hymenoptera: Proctotrupidae), Uga menoni (Kerrich), (Hymenoptera: Cha1cididae), and Pediobius foveolatus (Crawford), (Hymenoptera: Eulophidae) were reared from the host insects. A solitary larval parasitoid, N. afissae, was reared from 2nd to 4th instar brval hosts, with parasitism being highest in the 3rd instar collection. U. menoni, a solitary larval-pupal parasitoid, was reared from 3rd instar larvae to pupae, and parasitism was highest in the pupal collection. N. afissae and U. menoni occurred during June to September. N. afissae showed relatively high parasitism during the entire study period as compared with other species, while parasitization by U. menoni was highest in July. P. foveolatus, a gregarious larval parasitoid, did not generally appear until September, although it appeared in early August in the southern part of Korea. The number of P. foveolatus emerging from a mummy varied, ranging from 7 to 26 (mean = 13. 8).
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곤충기생성 선충, Steinernema feltiae와 Heterorhabditis heliothis를 이용한 고자리파리 및 담배거세미나방의 생물적 방제
추호렬,Harry K. Kaya,David K. Reed 한국응용곤충학회 1988 한국응용곤충학회지 Vol.27 No.4
Laboratory trials were conducted for control of onion maggot and tobacco cutworm with entomogenous nematodes. The onion maggot, Delia antiqua, was exposed to Steinernema feltiae at concentration of 0,30,60, 120 or 240 nematodes per larva, and to Heterorhabditis heliot-hidis at concentration of 0, 10, 20, 40 or 80 nematodes per larva. Mortalities of the maggot ranged from 80 to 100% in S. feltiae and from 63.3 to 100% in H. heliothidis. The tobacco cutworm, SpodoPtera litura was exposed to S. feltiae at concentration of 0,50,100,200 or 400 nematodes per larva and to H. heliothidis at concentration of 0,20,40,80 or 160 nematodes per larva with or without kale in petri dish. The 3rd instar larvae of the tobacco cutworm was more susceptible to both nematode species than the 4th or 5th instar at low concentration. Mortalities of the 3rd instar were 100% in S. feltiae and 67.7-100% in H. heliothidis while those of 4th and 5th instar ranged 76.7-100% and 43.3-100% in S. feltiae, and 36.7-90% and 3.3-90% in H. heliothidis, respectively. Mortalities of the tobacco cutworm larvae decreased when the nematodes were sprayed on the kale leaves in petri dish except 3rd instar. 고자리파리와 담배거세미나방을 방제할 목적으로 곤충기생성 선충, S. feltiae와 H. heliothidis를 이용, 실내 실험한 결과는 다음과 같다. 고자리 파리에서 S. feltiae를 유충 한 마리당 30,60,120,240마리를 접종한 모든 구에서 80-100%의 높은 치사율을 얻었고, H. heliothidis 10,20,40,80마리 접종 구에서도 63.3-100%치사율을 얻었다. 담배거세미나방의 경우 S. feltiae는 유충한마리당 50,100,200,400마리를 접종하여 3령층은 전 선충 접종구에서 100%의 치사율을, 4령충은 76.7-100%, 5령충은 43.3-100%의 치사율을 나타내어 접종량과 령게에 따라 치아율에 차이를 보였다. H. heliothidis는 20,40,80,160마리를 접종하여 3령층에서는 67.7-100%의 높은 치사율을 보였으나 4령충과 5령충에서는 36.7-90%, 3.3-90%의 치사율을 각각 나타내었다. 먹이인 케일에 처리한 경우 S. feltiae는 비교적 효과가 있었으나 H. heliothidis는 효과가 현저히 검소하였다. 그러나 3령충에서는 효과가 있어 3령충이 가장 방제 적기이며 감수적 영기임이 확인되었다.
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Manganese sulfide formation via concomitant microbial manganese oxide and thiosulfate reduction
Lee, Ji‐,Hoon,Kennedy, David W.,Dohnalkova, Alice,Moore, Dean A.,Nachimuthu, Ponnusamy,Reed, Samantha B.,Fredrickson, James K. Blackwell Publishing Ltd 2011 Environmental microbiology Vol.13 No.12
<P><B>Summary</B></P><P>The dissimilatory metal‐reducing bacterium, <I>Shewanella oneidensis</I> MR‐1 produced γ‐MnS (rambergite) nanoparticles during the concurrent reduction of MnO<SUB>2</SUB> and thiosulfate coupled to H<SUB>2</SUB> oxidation. To investigate effect of direct microbial reduction of MnO<SUB>2</SUB> on MnS formation, two MR‐1 mutants defective in outer membrane <I>c</I>‐type cytochromes (Δ<I>mtrC</I>/Δ<I>omcA</I> and Δ<I>mtrC</I>/Δ<I>omcA</I>/Δ<I>mtrF</I>) were also used and it was determined that direct reduction of MnO<SUB>2</SUB> was dominant relative to chemical reduction by biogenic sulfide generated from thiosulfate reduction. Although bicarbonate was excluded from the medium, incubations of strain MR‐1 with lactate as the electron donor produced MnCO<SUB>3</SUB> (rhodochrosite) as well as MnS in nearly equivalent amounts as estimated by micro X‐ray diffraction (micro‐XRD) analysis. It was concluded that carbonate released from lactate metabolism promoted MnCO<SUB>3</SUB> formation and that Mn(II) mineralogy was strongly affected by carbonate ions even in the presence of abundant sulfide and weakly alkaline conditions expected to favour the precipitation of MnS. Formation of MnS, as determined by a combination of micro‐XRD, transmission electron microscopy, energy dispersive X‐ray spectroscopy, and selected area electron diffraction analyses was consistent with equilibrium speciation modelling predictions. Biogenic manganese sulfide may be a manganese sink in the Mn biogeochemical cycle in select environments such as deep anoxic marine basins within the Baltic Sea.</P>
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추호렬,우건석,이영인,문수호,Choo, H. Y.,Woo, K. S.,Reed, David K.,Lee, Y. I.,Moon, S. H. 한국응용곤충학회 1988 한국응용곤충학회지 Vol.27 No.1
Phytophagous insects were surveyed for biological control of weeds in the southern part of korea from April 20 to July 31, 1987. Phytophagous coleopteran insects representing 12 families, 52 genera and 61 species, hemipteran insects 7 families, 18 genera and 20 species, homopteran insects 4 families, 16 genera and 21 species, hymenopteran insects 2 families, 2 genera and 3 species, and orthopteran 2 familes, 6 genera and 6 species were recorded from 58 weeds. Some chrysomelids and orthopteran insects were potential agents for biological control of weeds.
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