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- 저자 : Rahimmalek, Mehdi (검색결과 5 건)
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Mehdi Rahimmalek,Badraldin Ebrahim Sayed Tabatabaei,Ahmad Arzani,Mojtaba Khorrami 한국유전학회 2011 Genes & Genomics Vol.33 No.5
Yarrow (Achillea millefoilum L.) is an important medicinal plant with different medicinal and ornamental uses. In this study, SSR markers were developed for the first time from the genome A. millefolium using the slightly modified Hamilton protocol. Three yarrow genomic libraries were constructed,enriched for microsatellite motifs AG, AC and ATG. A total of 30 primer pairs were developed of which 16 were polymorphic in 26 A. millefolium accessions. One accession from A. tenuifolia species was also included to assess the transferability of new developed SSR primers in other species. The average allele number of SSR markers was 8.5 per locus and ranged from 2 to 14. The observed heterozygosity (HO) varied from 0 to 0.96 with an average of 0.52 and the expected heterozygosity (H_E) ranged from 0.07 to 0.49 with an average of 0.39. Among primers, Am142 showed the highest polymorphic information content (PIC) and the lowest value was obtained from Am59 primer with an average of 0.33. Three loci (AmK59, AmK344 and AmK329) deviated significantly from Hardy-Wrinberg Equlibrium (HWE) and one locus (Am344)might have null alleles. Cluster and PCoA analyses classified A. millefolium accessions according to their geographical distribution and A. tenuifolia species completely separated from A. millefoliulm genotypes.
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Mahboobeh Hodaei,Mehdi Rahimmalek,Ahmad Arzani 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.5
Knowledge of genetic diversity and relatedness of cultivars; is essential for a successful breeding scheme. The present studywas conducted to assess the genetic diversity of 30 Iranian Chrysanthemum morifolium cultivars using sequence-relatedamplifi ed polymorphism (SRAP) molecular markers and morphological traits. For this purpose, 30 cultivars were plantedin a completely randomized block design with three replications at the Research Farm of Isfahan University of Technologyin 2015. The evaluation of morphological traits during the two consecutive years showed a wide range of variationsamong diff erent cultivars. The results obtained by the analysis of variance also revealed the signifi cant eff ects of cultivar,environment and cultivar × environment interaction on all studied traits. Correlation analysis of variables further showedthat fl owering durability was aff ected by plant morphology. Results of cluster analysis based on the phenotypic data dividedthe cultivars into six groups. Molecular analysis using SRAP markers produced a total of 213 identifi able fragments withan average of 21.3 polymorphic bands per combination. The polymorphic information content (PIC) value was 0.46. Alongwith the Bayesian structure analysis which showed that the highest value of the maximum delta log likelihood (ΔK) wasK = 3, cluster analysis also grouped the cultivars into three main clusters. The high value of admixture revealed ancestralrelationships between the studied cultivars. Overall, our fi ndings revealed the potential of SRAP markers and morphologicaltraits for the analysis of genetic diversity in Chrysanthemum. This can be, in turn, used in selecting the desired parents in thehybrid breeding programs as well as developing superior cultivars for ornamental applications.
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Talebi, Majid,Hajiahmadi, Zahra,Rahimmalek, Mehdi 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
In the present study, genetic diversity of 48 individual plants from four Iranian cultivated populations of alfalfa (Medicago sativa L.) was evaluated using sequence-related amplified polymorphism (SRAP) markers. Fourteen SRAP primer combinations produced 193 fragments of which 95 were polymorphic. The number of polymorphic fragments detected per primer combination ranged from 3 to 10 bands with an average of 6.78 bands. Average polymorphic information content (PIC) was 0.343 for all primer combinations. Although the AMOVA (analysis of molecular variance) results showed a significant difference in the genetic diversity among the populations (P < 0.0001), the genetic variation mainly caused by the variation of intra population accounted for 93.17% of the total genetic variation. Unweighted pair-group method arithmetic average (UPGMA) analysis of the marker data clearly separated the populations of subtropical (Yazdi) and semi-cold (Hamadani and Nikshahri) as well as Kodi, an improved population. It can be concluded that SRAP markers are useful for studying diversity and relationships among and within alfalfa populations.
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Majid Talebi,Zahra Hajiahmadi,Mehdi Rahimmalek 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
In the present study, genetic diversity of 48 individual plants from four Iranian cultivated populations of alfalfa (Medicago sativa L.) was evaluated using sequence-related amplified polymorphism (SRAP) markers. Fourteen SRAP primer combinations produced 193 fragments of which 95 were polymorphic. The number of polymorphic fragments detected per primer combination ranged from 3 to 10 bands with an average of 6.78 bands. Average polymorphic information content (PIC) was 0.343 for all primer combinations. Although the AMOVA (analysis of molecular variance) results showed a significant difference in the genetic diversity among the populations (P < 0.0001), the genetic variation mainly caused by the variation of intra population accounted for 93.17% of the total genetic variation. Unweighted pair-group method arithmetic average (UPGMA) analysis of the marker data clearly separated the populations of subtropical (Yazdi) and semi-cold (Hamadani and Nikshahri) as well as Kodi, an improved population. It can be concluded that SRAP markers are useful for studying diversity and relationships among and within alfalfa populations.
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